PMID- 29284752
OWN - NLM
STAT- MEDLINE
DCOM- 20180717
LR  - 20181113
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 115
IP  - 2
DP  - 2018 Jan 9
TI  - Role of enhanced receptor engagement in the evolution of a pandemic acute 
      hemorrhagic conjunctivitis virus.
PG  - 397-402
LID - 10.1073/pnas.1713284115 [doi]
AB  - Acute hemorrhagic conjunctivitis (AHC) is a painful, contagious eye disease, with 
      millions of cases in the last decades. Coxsackievirus A24 (CV-A24) was not 
      originally associated with human disease, but in 1970 a pathogenic "variant" 
      (CV-A24v) emerged, which is now the main cause of AHC. Initially, this variant 
      circulated only in Southeast Asia, but it later spread worldwide, accounting for 
      numerous AHC outbreaks and two pandemics. While both CV-A24 variant and 
      nonvariant strains still circulate in humans, only variant strains cause AHC for 
      reasons that are yet unknown. Since receptors are important determinants of viral 
      tropism, we set out to map the CV-A24 receptor repertoire and establish whether 
      changes in receptor preference have led to the increased pathogenicity and rapid 
      spread of CV-A24v. Here, we identify ICAM-1 as an essential receptor for both 
      AHC-causing and non-AHC strains. We provide a high-resolution cryo-EM structure 
      of a virus-ICAM-1 complex, which revealed critical ICAM-1-binding residues. These 
      data could help identify a possible conserved mode of receptor engagement among 
      ICAM-1-binding enteroviruses and rhinoviruses. Moreover, we identify a single 
      capsid substitution that has been adopted by all pandemic CV-A24v strains and we 
      reveal that this adaptation enhances the capacity of CV-A24v to bind sialic acid. 
      Our data elucidate the CV-A24v receptor repertoire and point to a role of 
      enhanced receptor engagement in the adaptation to the eye, possibly enabling 
      pandemic spread.
FAU - Baggen, Jim
AU  - Baggen J
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - Hurdiss, Daniel L
AU  - Hurdiss DL
AUID- ORCID: 0000-0003-3834-5808
AD  - Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds LS2 9JT, United Kingdom.
FAU - Zocher, Georg
AU  - Zocher G
AD  - Interfaculty Institute of Biochemistry, University of Tubingen, 72076 Tubingen, 
      Germany.
FAU - Mistry, Nitesh
AU  - Mistry N
AD  - Division of Virology, Department of Clinical Microbiology, Umea University, 90187 
      Umea, Sweden.
FAU - Roberts, Richard W
AU  - Roberts RW
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - Slager, Jasper J
AU  - Slager JJ
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - Guo, Hongbo
AU  - Guo H
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - van Vliet, Arno L W
AU  - van Vliet ALW
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - Wahedi, Maryam
AU  - Wahedi M
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - Benschop, Kimberley
AU  - Benschop K
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment, 3720BA Bilthoven, The Netherlands.
FAU - Duizer, Erwin
AU  - Duizer E
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment, 3720BA Bilthoven, The Netherlands.
FAU - de Haan, Cornelis A M
AU  - de Haan CAM
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - de Vries, Erik
AU  - de Vries E
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - Casasnovas, Jose M
AU  - Casasnovas JM
AD  - Department of Macromolecular Structures, Centro Nacional de Biotecnologia, Campus 
      Universidad Autonoma de Madrid, 28049 Madrid, Spain.
FAU - de Groot, Raoul J
AU  - de Groot RJ
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - Arnberg, Niklas
AU  - Arnberg N
AUID- ORCID: 0000-0002-7069-6678
AD  - Division of Virology, Department of Clinical Microbiology, Umea University, 90187 
      Umea, Sweden.
FAU - Stehle, Thilo
AU  - Stehle T
AD  - Interfaculty Institute of Biochemistry, University of Tubingen, 72076 Tubingen, 
      Germany.
FAU - Ranson, Neil A
AU  - Ranson NA
AUID- ORCID: 0000-0002-3640-5275
AD  - Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds LS2 9JT, United Kingdom.
FAU - Thibaut, Hendrik Jan
AU  - Thibaut HJ
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
FAU - van Kuppeveld, Frank J M
AU  - van Kuppeveld FJM
AD  - Virology Division, Department of Infectious Diseases and Immunology, Faculty of 
      Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands; 
      F.J.M.vanKuppeveld@uu.nl.
LA  - eng
SI  - GENBANK/MG272373
SI  - GENBANK/MG272374
SI  - GENBANK/MG272375
SI  - PDB/6EIT
GR  - Wellcome Trust/United Kingdom
GR  - 102572/B/13/Z/Wellcome Trust/United Kingdom
GR  - 108466/Z/15/Z/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171228
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Capsid Proteins)
RN  - 0 (Receptors, Virus)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Capsid Proteins/genetics/metabolism
MH  - Conjunctivitis, Acute Hemorrhagic/epidemiology/*metabolism/virology
MH  - Cryoelectron Microscopy
MH  - Disease Outbreaks
MH  - Enterovirus C, Human/genetics/*metabolism/physiology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/chemistry/*metabolism
MH  - Mutation
MH  - N-Acetylneuraminic Acid/metabolism
MH  - Pandemics
MH  - Phylogeny
MH  - Protein Binding
MH  - Receptors, Virus/chemistry/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Viral Tropism/physiology
PMC - PMC5777043
OTO - NOTNLM
OT  - ICAM-1
OT  - conjunctivitis
OT  - coxsackievirus A24v
OT  - receptor
OT  - sialic acid
COIS- The authors declare no conflict of interest.
EDAT- 2017/12/30 06:00
MHDA- 2018/07/18 06:00
PMCR- 2018/07/09
CRDT- 2017/12/30 06:00
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2018/07/18 06:00 [medline]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2018/07/09 00:00 [pmc-release]
AID - 1713284115 [pii]
AID - 201713284 [pii]
AID - 10.1073/pnas.1713284115 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):397-402. doi: 
      10.1073/pnas.1713284115. Epub 2017 Dec 28.