PMID- 29285124
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 14
IP  - 6
DP  - 2017 Dec
TI  - Rhein lysinate protects renal function in diabetic nephropathy of KK/HlJ mice.
PG  - 5801-5808
LID - 10.3892/etm.2017.5283 [doi]
AB  - The purpose of the present study was to assess the protective effects of rhein 
      lysinate (RHL) in a KK/HlJ mouse model of diabetic nephropathy (DN) and to 
      explore its mechanism of action. A total of 4 groups were established: C57BL/J 
      control, the KK/HlJ model and 25 and 50 mg/kg/day RHL-treated KK/HlJ groups. The 
      KK/HlJ mouse model of DN was established by streptozotocin injection, followed by 
      maintenance on a specific diet. The albumin-to-creatinine ratio (ACR) was 
      determined at 5 weeks and at 16 weeks, the kidneys were harvested, and 
      morphological examination and immunohistochemical analysis were performed. The 
      levels of malondialdehyde (MDA), as well as superoxide dismutase (SOD) and 
      glutathione peroxidase (GSH-px) activities in the kidneys were measured using 
      appropriate assay kits. The expression of inflammatory factors and associated 
      proteins was analyzed using western blot analysis. At 5 weeks, the levels of ACR 
      in KK/HlJ mice were increased, which was inhibited by treatment with RHL. 
      Treatment with RHL (50 mg/kg/day) decreased the body weight of KK/HlJ mice. 
      Compared with the C57BL/J control, the KK/HlJ model mice had a significantly 
      lower activity of SOD and GSH-px in the kidneys, but had significantly higher 
      levels of MDA. Treatment of KK/HlJ mice with RHL significantly increased the 
      activities SOD and GSH-px, and reduced the MAD level in the kidneys. Renal 
      tubular epithelial cell edema was observed in KK/HlJ mice but not in C57BL/J 
      mice. RHL decreased the incidence of renal tubular epithelial cell edema and 
      significantly decreased the expression of TNF-alpha and IL-6 as well as the 
      expression and phosphorylation of NF-kappaB in the kidneys. Therefore, DN is 
      associated with the expression of inflammatory factors, renal tubular epithelial 
      cell edema and renal dysfunction in KK/HlJ mice. RHL improves renal function by 
      decreasing kidney inflammation.
FAU - Lin, Ya-Jun
AU  - Lin YJ
AD  - The Key Laboratory of Geriatrics, Beijing Hospital, National Center of 
      Gerontology, Beijing 100730, P.R. China.
FAU - Zhen, Yong-Zhan
AU  - Zhen YZ
AD  - Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for 
      Preclinical and Basic Research on Chronic Diseases, School of Basic Medical 
      Sciences, North China University of Science and Technology, Tangshan, Hebei 
      063000, P.R. China.
FAU - Wei, Jing-Bo
AU  - Wei JB
AD  - Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for 
      Preclinical and Basic Research on Chronic Diseases, School of Basic Medical 
      Sciences, North China University of Science and Technology, Tangshan, Hebei 
      063000, P.R. China.
FAU - Wei, Jie
AU  - Wei J
AD  - The Key Laboratory of Geriatrics, Beijing Hospital, National Center of 
      Gerontology, Beijing 100730, P.R. China.
FAU - Dai, Jing
AU  - Dai J
AD  - Department of Endocrinology, Peking University Fifth School of Clinical Medicine, 
      Beijing Hospital, Beijing 100730, P.R. China.
FAU - Gao, Jun-Ling
AU  - Gao JL
AD  - Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for 
      Preclinical and Basic Research on Chronic Diseases, School of Basic Medical 
      Sciences, North China University of Science and Technology, Tangshan, Hebei 
      063000, P.R. China.
FAU - Li, Kai-Ji
AU  - Li KJ
AD  - Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for 
      Preclinical and Basic Research on Chronic Diseases, School of Basic Medical 
      Sciences, North China University of Science and Technology, Tangshan, Hebei 
      063000, P.R. China.
FAU - Hu, Gang
AU  - Hu G
AD  - The Key Laboratory of Geriatrics, Beijing Hospital, National Center of 
      Gerontology, Beijing 100730, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171011
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5740561
OTO - NOTNLM
OT  - KK/HlJ mice
OT  - inflammatory factors
OT  - kidney
OT  - oxidative stress
OT  - rhein lysinate
EDAT- 2017/12/30 06:00
MHDA- 2017/12/30 06:01
PMCR- 2017/10/11
CRDT- 2017/12/30 06:00
PHST- 2016/02/18 00:00 [received]
PHST- 2017/08/14 00:00 [accepted]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2017/12/30 06:01 [medline]
PHST- 2017/10/11 00:00 [pmc-release]
AID - ETM-0-0-5283 [pii]
AID - 10.3892/etm.2017.5283 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Dec;14(6):5801-5808. doi: 10.3892/etm.2017.5283. Epub 2017 Oct 
      11.