PMID- 29285151
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 14
IP  - 6
DP  - 2017 Dec
TI  - Protective effects of notoginsenoside R1 on cerebral ischemia-reperfusion injury 
      in rats.
PG  - 6012-6016
LID - 10.3892/etm.2017.5268 [doi]
AB  - The objective of this study was to investigate the protective effect of 
      notoginsenoside R1 (NGR1) on cerebral ischemia-reperfusion injury (CIRI) in rats, 
      and its molecular mechanism, to provide new insights into the diagnosis and 
      treatment of CIRI. Sixty Sprague-Dawley rats were randomly divided into four 
      groups including the sham-operation group (Sham), cerebral ischemia-reperfusion 
      model group (CIR), NGR1 treatment group (NGR1), and nimodipine positive control 
      group (NDC) with 15 rats each. Bilateral common carotid arteries occlusion was 
      used to establish the rat CIRI model. The area of cerebral infarction at the end 
      of reperfusion was calculated by triphenyl tetrazolium chloride staining. 
      Apoptosis of hippocampal neurons in each group was detected by Annexin 
      V/propidium iodide double staining. Hippocampal expression of brain-derived 
      neurotrophic factor (BDNF) mRNA, and Bcl-2 and Bax protein at the end of 
      reperfusion were measured by RT-qPCR and western blot analysis, respectively. 
      Data were analyzed by SPSS software analysis to ensure statistical significance. 
      At the end of reperfusion, the area of cerebral infarction in the NGR1 and NDC 
      groups was significantly smaller than that of the CIR group. Apoptosis analysis 
      showed that compared with the CIR group, the apoptosis rate of hippocampal 
      neurons was significantly decreased in the NGR1 and NDC groups. RT-qPCR and 
      western blot analysis showed that at the end of reperfusion, higher levels of 
      BDNF mRNA and the anti-apoptotic factor, Bcl-2, and lower levels of the 
      pro-apoptotic factor, Bax, in the hippocampus were found in the NGR1 and NDC 
      groups compared with the CIR group. The protective effect of NGR1 on CIRI was 
      significantly stronger than that of nimodipine. In conclusion, NGR1 can reduce 
      the area of cerebral infarction, reduce apoptosis of hippocampal neurons, and 
      protect rats from CIRI. Those effects were achieved by activating the expression 
      of BDNF and Bcl-2, and by inhibiting the expression of Bax.
FAU - Zou, Shun
AU  - Zou S
AD  - Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical 
      University, Kunming, Yunnan 650101, P.R. China.
FAU - Zhang, Mingxiong
AU  - Zhang M
AD  - Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical 
      University, Kunming, Yunnan 650101, P.R. China.
FAU - Feng, Limei
AU  - Feng L
AD  - Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical 
      University, Kunming, Yunnan 650101, P.R. China.
FAU - Zhou, Yuanfang
AU  - Zhou Y
AD  - Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical 
      University, Kunming, Yunnan 650101, P.R. China.
FAU - Li, Li
AU  - Li L
AD  - Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical 
      University, Kunming, Yunnan 650101, P.R. China.
FAU - Ban, Lili
AU  - Ban L
AD  - Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical 
      University, Kunming, Yunnan 650101, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171006
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5740559
OTO - NOTNLM
OT  - Bax
OT  - Bcl-2
OT  - brain-derived neurotrophic factor
OT  - cerebral ischemia-reperfusion injury
OT  - notoginsenoside R1
EDAT- 2017/12/30 06:00
MHDA- 2017/12/30 06:01
PMCR- 2017/10/06
CRDT- 2017/12/30 06:00
PHST- 2017/05/25 00:00 [received]
PHST- 2017/09/21 00:00 [accepted]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2017/12/30 06:01 [medline]
PHST- 2017/10/06 00:00 [pmc-release]
AID - ETM-0-0-5268 [pii]
AID - 10.3892/etm.2017.5268 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Dec;14(6):6012-6016. doi: 10.3892/etm.2017.5268. Epub 2017 Oct 
      6.