PMID- 29285153
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 14
IP  - 6
DP  - 2017 Dec
TI  - Effects of quercetin on the expression of MCP-1, MMP-9 and VEGF in rats with 
      diabetic retinopathy.
PG  - 6022-6026
LID - 10.3892/etm.2017.5275 [doi]
AB  - Diabetic retinopathy, a severe complication of diabetes, is the leading cause of 
      blindness in the developed world. This study investigated the effects of 
      quercetin on levels of monocyte chemoattractant protein-1 (MCP-1), matrix 
      metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) in 
      serum of rats with diabetic retinopathy, and explored the functional mechanisms 
      of quercetin in the treatment of diabetic retinopathy. Twenty rats with induced 
      diabetes were divided into a model group and a quercetin group, with 10 rats in 
      each group. Ten healthy rats were also included to serve as a control group. Rats 
      in the quercetin group were treated with an intragastric injection of quercetin 
      (150 mg/kg), while the same amount of sodium carboxymethyl cellulose (CMCNa) was 
      used for rats in the model group and the control group. The treatment was 
      performed once per day and blood glucose was measured in each group at 0, 10 and 
      20 weeks after the first treatment. Blood glucose tests showed that quercetin did 
      not reduce blood glucose in rats with diabetes. However, pathological examination 
      showed that quercetin could relieve pathological changes caused by diabetes, such 
      as retinal edema and vacuoles. ELISA results showed that, compared with the 
      control group, levels of MCP-1, MMP-9 and VEGF in the model group were 
      significantly increased (P<0.01). No significant difference in serum MCP-1 
      content was found between the model group and the quercetin group, but levels of 
      MMP-9 and VEGF were significantly decreased in the quercetin group (P<0.01). 
      Results of RT-PCR and western blot analysis showed that, compared with the 
      control group, levels of MCP-1, MMP-9 and VEGF mRNA and protein in the retinal 
      tissue of rats in the model group were significantly increased (P<0.01). No 
      significant differences in expression levels of MCP-1 mRNA and protein were found 
      between the model group and the quercetin group, but levels of MMP-9 and VEGF 
      mRNA and protein were significantly decreased in the quercetin group (P<0.01). 
      Quercetin has a certain therapeutic effect on rats with diabetic retinopathy and 
      its effect may be achieved by reducing the expression of MMP-9 and VEGF, but not 
      the inflammatory mediator, MCP-1.
FAU - Chen, Bin
AU  - Chen B
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China.
FAU - He, Tao
AU  - He T
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China.
FAU - Xing, Yiqiao
AU  - Xing Y
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China.
FAU - Cao, Ting
AU  - Cao T
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171010
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5740807
OTO - NOTNLM
OT  - diabetic retinopathy
OT  - matrix metalloproteinase-9
OT  - monocyte chemo-attractant protein-1
OT  - quercetin
OT  - vascular endothelial growth factor
EDAT- 2017/12/30 06:00
MHDA- 2017/12/30 06:01
PMCR- 2017/10/10
CRDT- 2017/12/30 06:00
PHST- 2017/06/02 00:00 [received]
PHST- 2017/09/27 00:00 [accepted]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2017/12/30 06:01 [medline]
PHST- 2017/10/10 00:00 [pmc-release]
AID - ETM-0-0-5275 [pii]
AID - 10.3892/etm.2017.5275 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Dec;14(6):6022-6026. doi: 10.3892/etm.2017.5275. Epub 2017 Oct 
      10.