PMID- 29285171
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 14
IP  - 6
DP  - 2017 Dec
TI  - MicroRNA-10a-5p suppresses cancer proliferation and division in human cervical 
      cancer by targeting BDNF.
PG  - 6147-6151
LID - 10.3892/etm.2017.5312 [doi]
AB  - The aim of the present study was to investigate the effect and mechanism of 
      microRNA (miR)-10a-5p in human cervical cancer. The expression level of miR-10-5p 
      in cervical cancer lines was assessed using reverse transcription-quantitative 
      polymerase chain reaction (RT-qPCR). In cervical cancer HeLa and SiHa cells, 
      miR-10-5p was ectopically overexpressed by lentiviral transduction. Brain-derived 
      neurotrophic factor (BDNF) was then overexpressed in HeLa and SiHa cells to 
      evaluate its selective effect on miR-10-5p in cervical cancer modulation. The 
      targeting of miR-10-5p on its downstream gene, BDNF, was evaluated using RT-qPCR 
      and western blot analysis. Cervical cancer cell viability and cell cycle was 
      evaluated using an MTT assay and flow cytometry, respectively. The results 
      indicated that miR-10-5p expression was significantly lower in cervical cancer 
      cell lines compared with normal cells (P<0.05). Ectopic overexpression of 
      miR-10-5p significantly inhibited cancer cell viability and induced cell cycle 
      arrest in HeLa and SiHa cells (both P<0.05). miR-10-5p overexpression 
      significantly reduced BDNF gene expression (P<0.05) and also reduced BDNF protein 
      levels in cervical cancer cells compared with the control. In conclusion, the 
      current study indicated that miR-10-5p is a cervical cancer suppressor, which 
      regulates BDNF expression in cervical cancer.
FAU - Zhai, Liancheng
AU  - Zhai L
AD  - Laboratory Department, Maternal and Child Health Care Hospital of Zhengzhou, 
      Zhengzhou, Henan 450000, P.R. China.
FAU - Li, Yanli
AU  - Li Y
AD  - Laboratory Department, Maternal and Child Health Care Hospital of Zhengzhou, 
      Zhengzhou, Henan 450000, P.R. China.
FAU - Lan, Xinzhi
AU  - Lan X
AD  - Laboratory Department, Maternal and Child Health Care Hospital of Zhengzhou, 
      Zhengzhou, Henan 450000, P.R. China.
FAU - Ai, Liang
AU  - Ai L
AD  - Laboratory Department, Maternal and Child Health Care Hospital of Zhengzhou, 
      Zhengzhou, Henan 450000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171016
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5740563
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - cervical cancer
OT  - microRNA-10a-5p
EDAT- 2017/12/30 06:00
MHDA- 2017/12/30 06:01
PMCR- 2017/10/16
CRDT- 2017/12/30 06:00
PHST- 2016/05/16 00:00 [received]
PHST- 2017/03/24 00:00 [accepted]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2017/12/30 06:01 [medline]
PHST- 2017/10/16 00:00 [pmc-release]
AID - ETM-0-0-5312 [pii]
AID - 10.3892/etm.2017.5312 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Dec;14(6):6147-6151. doi: 10.3892/etm.2017.5312. Epub 2017 Oct 
      16.