PMID- 29285315
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 62
DP  - 2017 Dec 1
TI  - CXCL8 derived from tumor-associated macrophages and esophageal squamous cell 
      carcinomas contributes to tumor progression by promoting migration and invasion 
      of cancer cells.
PG  - 106071-106088
LID - 10.18632/oncotarget.22526 [doi]
AB  - Tumor-associated macrophages (TAMs) are involved in tumor progression and poor 
      prognosis in several malignancies. We previously demonstrated the interaction 
      between high numbers of infiltrating TAMs and poor prognosis in esophageal 
      squamous cell carcinomas (ESCCs). To investigate the significance of TAMs in 
      ESCC, we conducted a cDNA microarray analysis of peripheral blood monocytes 
      (PBMo)-derived macrophages and PBMo-derived macrophages stimulated with 
      conditioned media of TE-series ESCC cell lines (TAM-like PBMo-derived 
      macrophages). C-X-C motif chemokine ligand 8 (CXCL8) was up-regulated in the 
      TAM-like PBMo-derived macrophages. Here we confirmed a high expression level of 
      CXCL8 in TAM-like PBMo-derived macrophages and the expression of CXCR1/2, known 
      as CXCL8 receptors, in TE-series ESCC cell lines. Recombinant human CXCL8 induced 
      the ESCC cell lines' migration and invasion by the phosphorylation of Akt and 
      Erk1/2. In indirect co-cultures, not only signal pathway inhibitors but also 
      neutralizing antibodies against CXCL8, CXCR1 and CXCR2 suppressed these 
      phenotypes induced by TAM-like PBMo-derived macrophages. Immunohistochemical 
      analysis of 70 resected ESCC samples showed that high expression levels of CXCL8 
      in ESCC tissues were significantly associated with lymph node metastasis and poor 
      prognosis. These results suggest that CXCL8 up-regulated in the microenvironment 
      may contribute to ESCC progression by promoting cancer cells' migration and 
      invasion.
FAU - Hosono, Masayoshi
AU  - Hosono M
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
AD  - Division of Gastro-intestinal Surgery, Department of Surgery, Kobe University 
      Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Koma, Yu-Ichiro
AU  - Koma YI
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Takase, Nobuhisa
AU  - Takase N
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
AD  - Division of Gastro-intestinal Surgery, Department of Surgery, Kobe University 
      Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Urakawa, Naoki
AU  - Urakawa N
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
AD  - Division of Gastro-intestinal Surgery, Department of Surgery, Kobe University 
      Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Higashino, Nobuhide
AU  - Higashino N
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
AD  - Division of Gastro-intestinal Surgery, Department of Surgery, Kobe University 
      Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Suemune, Kazuki
AU  - Suemune K
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Kodaira, Himiko
AU  - Kodaira H
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Nishio, Mari
AU  - Nishio M
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Shigeoka, Manabu
AU  - Shigeoka M
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Kakeji, Yoshihiro
AU  - Kakeji Y
AD  - Division of Gastro-intestinal Surgery, Department of Surgery, Kobe University 
      Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
FAU - Yokozaki, Hiroshi
AU  - Yokozaki H
AD  - Division of Pathology, Department of Pathology, Kobe University Graduate School 
      of Medicine, Chuo-ku, Kobe 650-0017, Japan.
LA  - eng
PT  - Journal Article
DEP - 20171120
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
PMC - PMC5739702
OTO - NOTNLM
OT  - CXCL8
OT  - esophageal squamous cell carcinoma
OT  - tumor microenvironment
OT  - tumor progression
OT  - tumor-associated macrophage
COIS- CONFLICTS OF INTEREST The authors declare no conflicts of interest.
EDAT- 2017/12/30 06:00
MHDA- 2017/12/30 06:01
PMCR- 2017/12/01
CRDT- 2017/12/30 06:00
PHST- 2017/07/19 00:00 [received]
PHST- 2017/10/28 00:00 [accepted]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2017/12/30 06:01 [medline]
PHST- 2017/12/01 00:00 [pmc-release]
AID - 22526 [pii]
AID - 10.18632/oncotarget.22526 [doi]
PST - epublish
SO  - Oncotarget. 2017 Nov 20;8(62):106071-106088. doi: 10.18632/oncotarget.22526. 
      eCollection 2017 Dec 1.