PMID- 29286135
OWN - NLM
STAT- MEDLINE
DCOM- 20180828
LR  - 20181113
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 39
IP  - 3
DP  - 2018 Mar
TI  - Characterization of different osteoclast phenotypes in the progression of bone 
      invasion by oral squamous cell carcinoma.
PG  - 1043-1051
LID - 10.3892/or.2017.6166 [doi]
AB  - The present study aimed to characterize different phenotypes of osteoclasts in 
      the progression of bone invasion by oral squamous cell carcinoma (OSCC). A local 
      bone invasion model of OSCC was established by injecting SCC25 human OSCC cells 
      into the center of calvariae in nude mice, and all mice were found to have a 
      typical bone resorption area. Staining for tartrate-resistant acid phosphatase 
      (TRAP) revealed various types of giant osteoclasts in the tumour-bone interface. 
      Bone marrow cells (BMCs) were isolated from the nude mice for primary osteoclast 
      culture, but only a few giant osteoclasts were generated. Additionally, special 
      blood centrifuge tubes were utilized to obtain large numbers of peripheral blood 
      mononuclear cells (PBMCs). Using magnetic activated cell sorting (MACS) and the 
      cytokines colony-stimulating factor (CSF) and receptor activator of nuclear 
      factor-kappab ligand (RANKL), we differentiated human osteoclasts from CD14+ 
      monocytes of PBMCs. Bone resorption was further confirmed by a bone resorption 
      assay. Finally, Transwell inserts were used for indirect cell co-culture of SCC25 
      cells and CD14+ monocytes. Expression of specific osteoclast markers was detected 
      by real-time PCR and western blotting. After co-culture for 3 and 6 days, 
      conditioned medium (CM) of SCC25 cells stimulated the expression of osteoclast 
      markers, and additional osteoclasts were detected through staining of TRAP and 
      F-actin. In the present study distinct osteoclast phenotypes were observed in the 
      established bone invasion animal model, and were confirmed using various primary 
      osteoclast cultures. CM of OSCC cells may promote the expression of osteoclast 
      markers and induce the differentiation of monocytes to mature osteoclasts, which 
      can resorb adjacent bone tissue.
FAU - Quan, Jingjing
AU  - Quan J
AD  - Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University 
      and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 
      510080, P.R. China.
FAU - Hou, Yuluan
AU  - Hou Y
AD  - Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University 
      and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 
      510080, P.R. China.
FAU - Long, Weiling
AU  - Long W
AD  - Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University 
      and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 
      510080, P.R. China.
FAU - Ye, Shu
AU  - Ye S
AD  - Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University 
      and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 
      510080, P.R. China.
FAU - Wang, Zhiyuan
AU  - Wang Z
AD  - Affiliated High School-South China Normal University, Guangzhou, Guangdong 
      510630, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171219
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
SB  - IM
MH  - Animals
MH  - Bone Neoplasms/metabolism/*secondary
MH  - Bone Resorption/metabolism/*pathology
MH  - Carcinoma, Squamous Cell/metabolism/*secondary
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Humans
MH  - Leukocytes, Mononuclear/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Mouth Neoplasms/metabolism/*pathology
MH  - Osteoclasts/metabolism/*pathology
MH  - Phenotype
MH  - Xenograft Model Antitumor Assays
PMC - PMC5802026
EDAT- 2017/12/30 06:00
MHDA- 2018/08/29 06:00
PMCR- 2017/12/19
CRDT- 2017/12/30 06:00
PHST- 2017/06/18 00:00 [received]
PHST- 2017/12/05 00:00 [accepted]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2018/08/29 06:00 [medline]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/19 00:00 [pmc-release]
AID - or-39-03-1043 [pii]
AID - 10.3892/or.2017.6166 [doi]
PST - ppublish
SO  - Oncol Rep. 2018 Mar;39(3):1043-1051. doi: 10.3892/or.2017.6166. Epub 2017 Dec 19.