PMID- 29286384
OWN - NLM
STAT- MEDLINE
DCOM- 20180601
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 129
DP  - 2017 Nov 17
TI  - Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of 
      Atherosclerosis.
LID - 10.3791/56625 [doi]
LID - 56625
AB  - Atherosclerosis is a major contributor to cardiovascular disease, the leading 
      cause of death worldwide, which claims 17.3 million lives annually. 
      Atherosclerosis is also the leading cause of sudden death and myocardial 
      infarction, instigated by unstable plaques that rupture and occlude the blood 
      vessel without warning. Current imaging modalities cannot differentiate between 
      stable and unstable plaques that rupture. Peptide amphiphiles micelles (PAMs) can 
      overcome this drawback as they can be modified with a variety of targeting 
      moieties that bind specifically to diseased tissue. Monocytes have been shown to 
      be early markers of atherosclerosis, while large accumulation of monocytes is 
      associated with plaques prone to rupture. Hence, nanoparticles that can target 
      monocytes can be used to discriminate different stages of atherosclerosis. To 
      that end, here, we describe a protocol for the preparation of monocyte-targeting 
      PAMs (monocyte chemoattractant protein-1 (MCP-1) PAMs). MCP-1 PAMs are 
      self-assembled through synthesis under mild conditions to form nanoparticles of 
      15 nm in diameter with near neutral surface charge. In vitro, PAMs were found to 
      be biocompatible and had a high binding affinity for monocytes. The methods 
      described herein show promise for a wide range of applications in atherosclerosis 
      as well as other inflammatory diseases.
FAU - Poon, Christopher
AU  - Poon C
AD  - Department of Biomedical Engineering, University of Southern California.
FAU - Sarkar, Manjima
AU  - Sarkar M
AD  - Department of Biomedical Engineering, University of Southern California.
FAU - Chung, Eun Ji
AU  - Chung EJ
AD  - Department of Biomedical Engineering, University of Southern California; 
      eunchung@usc.edu.
LA  - eng
GR  - R00 HL124279/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20171117
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Micelles)
RN  - 0 (Peptides)
SB  - IM
MH  - Atherosclerosis/*diagnostic imaging/pathology
MH  - Humans
MH  - Micelles
MH  - Monocytes/*metabolism
MH  - Nanoparticles/*chemistry/*metabolism
MH  - Peptides/*chemical synthesis/*metabolism
PMC - PMC5755426
MID - NIHMS974064
EDAT- 2017/12/30 06:00
MHDA- 2018/06/02 06:00
PMCR- 2018/11/17
CRDT- 2017/12/30 06:00
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2018/06/02 06:00 [medline]
PHST- 2018/11/17 00:00 [pmc-release]
AID - 56625 [pii]
AID - 10.3791/56625 [doi]
PST - epublish
SO  - J Vis Exp. 2017 Nov 17;(129):56625. doi: 10.3791/56625.