PMID- 29286919
OWN - NLM
STAT- MEDLINE
DCOM- 20180827
LR  - 20220409
IS  - 1875-8592 (Electronic)
IS  - 1574-0153 (Linking)
VI  - 21
IP  - 3
DP  - 2018 Feb 14
TI  - Knockdown of lncRNA GHET1 suppresses cell proliferation, invasion and LATS1/YAP 
      pathway in non small cell lung cancer.
PG  - 557-563
LID - 10.3233/CBM-170431 [doi]
AB  - BACKGROUND: The aim in this study was to explore the role of long non-coding RNA 
      GHET1 in development of non small cell lung cancer (NSCLC). METHODS: LncRNA GHET1 
      expression levels were analyzed by qRT-PCR in tumor tissues and adjacent normal 
      tissues in NSCLC. Measuring the cell proliferation and invasion abilities by 
      CCK8, cell colony formation and transwell invasion assays. Relative protein 
      expression was analyzed by western blot assays. RESULTS: Expression of lncRNA 
      GHET1 was notably higher in NSCLC tissues compared with adjacent normal tissues 
      by using qRT-PCR analyses. Higher lncRNA GHET1 expression associated with lymph 
      node metastasis, TNM stage and showed poor outcome in NSCLC patients. Knockdown 
      of lncRNA GHET1 suppressed cell proliferation and invasion capacity and 
      Epithelial-Mesenchymal Transition (EMT) phenomenon of NSCLC cells. Moreover, we 
      demonstrated that knockdown of lncRNA GHET1 suppresses LATS1/YAP pathway 
      signaling pathway by downregulating YAP1 expression in NSCLC cells. CONCLUSIONS: 
      GHET1 predicted a poor outcome and acted as a tumor-promoting gene in NSCLC. 
      Thus, inhibition of GHET1 may be a potential target of NSCLC treatment.
FAU - Guan, Zhen-Biao
AU  - Guan ZB
FAU - Cao, Yu-Shu
AU  - Cao YS
FAU - Li, Yi
AU  - Li Y
FAU - Tong, Wan-Ning
AU  - Tong WN
FAU - Zhuo, An-Shan
AU  - Zhuo AS
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cancer Biomark
JT  - Cancer biomarkers : section A of Disease markers
JID - 101256509
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (GHET1 transcript, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Transcription Factors)
RN  - 0 (YY1AP1 protein, human)
RN  - EC 2.7.1.- (LATS1 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/*metabolism/mortality/pathology
MH  - Cell Cycle Proteins
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Lung Neoplasms/*genetics/*metabolism/pathology
MH  - Neoplasm Grading
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Nuclear Proteins/*metabolism
MH  - Prognosis
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - RNA, Long Noncoding/*genetics
MH  - Signal Transduction
MH  - Transcription Factors/*metabolism
OTO - NOTNLM
OT  - GHET1
OT  - Non small cell lung cancer
OT  - cell invasion
OT  - cell proliferation
OT  - long non-coding RNA
EDAT- 2017/12/30 06:00
MHDA- 2018/08/28 06:00
CRDT- 2017/12/30 06:00
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2018/08/28 06:00 [medline]
PHST- 2017/12/30 06:00 [entrez]
AID - CBM170431 [pii]
AID - 10.3233/CBM-170431 [doi]
PST - ppublish
SO  - Cancer Biomark. 2018 Feb 14;21(3):557-563. doi: 10.3233/CBM-170431.