PMID- 29287093
OWN - NLM
STAT- MEDLINE
DCOM- 20180207
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 12
DP  - 2017
TI  - Direct exposure to mild heat promotes proliferation and neuronal differentiation 
      of neural stem/progenitor cells in vitro.
PG  - e0190356
LID - 10.1371/journal.pone.0190356 [doi]
LID - e0190356
AB  - Heat acclimation in rats is associated with enhanced neurogenesis in 
      thermoregulatory centers of the hypothalamus. To elucidate the mechanisms for 
      heat acclimation, we investigated the effects of direct mild heat exposure on the 
      proliferation and differentiation of neural stem/progenitor cells (NSCs/NPCs). 
      The NSCs/NPCs isolated from forebrain cortices of 14.5-day-old rat fetuses were 
      propagated as neurospheres at either 37.0 degrees C (control) or 38.5 degrees C (mild heat 
      exposure) for four days, and the effects on proliferation were investigated by 
      MTS cell viability assay, measurement of neurosphere diameter, and counting the 
      total number of cells. The mRNA expressions of heat shock proteins (HSPs) and 
      brain-derived neurotrophic factor (BDNF), cAMP response element-binding (CREB) 
      protein and Akt phosphorylation levels, and intracellular reactive oxygen species 
      (ROS) levels were analyzed using real time PCR, Western blotting and CM-H2DCFDA 
      assay respectively. Heat exposure under proliferation condition increased NSC/NPC 
      viability, neurosphere diameter, and cell count. BDNF mRNA expression, CREB 
      phosphorylation, and ROS level were also increased by heat exposure. Heat 
      exposure increased HSP27 mRNA expression concomitant with enhanced p-Akt level. 
      Moreover, treatment with LY294002 (a PI3K inhibitor) abolished the effects of 
      heat exposure on NSC/NPC proliferation. Furthermore, heat exposure under 
      differentiation conditions increased the proportion of cells positive for Tuj1 (a 
      neuronal marker). These findings suggest that mild heat exposure increases 
      NSC/NPC proliferation, possibly through activation of the Akt pathway, and also 
      enhances neuronal differentiation. Direct effects of temperature on NSCs/NPCs may 
      be one of the mechanisms involved in hypothalamic neurogenesis in heat-acclimated 
      rats. Such heat-induced neurogenesis could also be an effective therapeutic 
      strategy for neurodegenerative diseases.
FAU - Hossain, Md Emon
AU  - Hossain ME
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
FAU - Matsuzaki, Kentaro
AU  - Matsuzaki K
AUID- ORCID: 0000-0002-5942-8840
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
FAU - Katakura, Masanori
AU  - Katakura M
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
AD  - Department of Nutritional Physiology, Faculty of Pharmaceutical Sciences, Josai 
      University, Sakado, Saitama, Japan.
FAU - Sugimoto, Naotoshi
AU  - Sugimoto N
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
AD  - Department of Physiology, Graduate School of Medical Science, Kanazawa 
      University, Kanazawa, Japan.
FAU - Mamun, Abdullah Al
AU  - Mamun AA
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
FAU - Islam, Rafiad
AU  - Islam R
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
FAU - Hashimoto, Michio
AU  - Hashimoto M
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
FAU - Shido, Osamu
AU  - Shido O
AD  - Department of Environmental Physiology, Faculty of Medicine, Shimane University, 
      Enya-cho, Izumo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171229
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chromones)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Morpholines)
RN  - 0 (Reactive Oxygen Species)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (Crebbp protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - CREB-Binding Protein/metabolism
MH  - *Cell Differentiation
MH  - *Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chromones/pharmacology
MH  - Heat-Shock Proteins/metabolism
MH  - *Hot Temperature
MH  - Morpholines/pharmacology
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Neurons/*cytology/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC5747471
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/12/30 06:00
MHDA- 2018/02/08 06:00
PMCR- 2017/12/29
CRDT- 2017/12/30 06:00
PHST- 2017/07/16 00:00 [received]
PHST- 2017/12/13 00:00 [accepted]
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2018/02/08 06:00 [medline]
PHST- 2017/12/29 00:00 [pmc-release]
AID - PONE-D-17-26463 [pii]
AID - 10.1371/journal.pone.0190356 [doi]
PST - epublish
SO  - PLoS One. 2017 Dec 29;12(12):e0190356. doi: 10.1371/journal.pone.0190356. 
      eCollection 2017.