PMID- 29290652
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20181202
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 23
IP  - 46
DP  - 2017 Dec 14
TI  - In vivo hepatic differentiation potential of human umbilical cord-derived 
      mesenchymal stem cells: Therapeutic effect on liver fibrosis/cirrhosis.
PG  - 8152-8168
LID - 10.3748/wjg.v23.i46.8152 [doi]
AB  - AIM: To investigate the hepatic differentiation potential of human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) and to evaluate their therapeutic 
      effect on liver fibrosis/cirrhosis. METHODS: A CCl(4)-induced liver 
      fibrotic/cirrhotic rat model was used to assess the effect of hUC-MSCs. 
      Histopathology was assessed by hematoxylin and eosin (H&E), Masson trichrome and 
      Sirius red staining. The liver biochemical profile was measured using a Beckman 
      Coulter analyzer. Expression analysis was performed using immunofluorescent 
      staining, immunohistochemistry, Western blot, and real-time PCR. RESULTS: We 
      demonstrated that the infused hUC-MSCs could differentiate into hepatocytes in 
      vivo. Functionally, the transplantation of hUC-MSCs to CCl(4)-treated rats 
      improved liver transaminases and synthetic function, reduced liver histopathology 
      and reversed hepatobiliary fibrosis. The reversal of hepatobiliary fibrosis was 
      likely due to the reduced activation state of hepatic stellate cells, decreased 
      collagen deposition, and enhanced extracellular matrix remodeling via the 
      up-regulation of MMP-13 and down-regulation of TIMP-1. CONCLUSION: Transplanted 
      hUC-MSCs could differentiate into functional hepatocytes that improved both the 
      biochemical and histopathologic changes in a CCl(4)-induced rat liver fibrosis 
      model. hUC-MSCs may offer therapeutic opportunities for treating hepatobiliary 
      diseases, including cirrhosis.
FAU - Zhang, Guo-Zun
AU  - Zhang GZ
AD  - Department of Gastroenterology, The Second Hospital of Hebei Medical University, 
      Shijiazhuang 050000, Hebei Province, China.
FAU - Sun, Hui-Cong
AU  - Sun HC
AD  - Department of Gastroenterology, The Second Hospital of Hebei Medical University, 
      Shijiazhuang 050000, Hebei Province, China.
FAU - Zheng, Li-Bo
AU  - Zheng LB
AD  - Department of Gastroenterology, The Second Hospital of Hebei Medical University, 
      Shijiazhuang 050000, Hebei Province, China.
FAU - Guo, Jin-Bo
AU  - Guo JB
AD  - Department of Gastroenterology, The Second Hospital of Hebei Medical University, 
      Shijiazhuang 050000, Hebei Province, China.
FAU - Zhang, Xiao-Lan
AU  - Zhang XL
AD  - Department of Gastroenterology, The Second Hospital of Hebei Medical University, 
      Shijiazhuang 050000, Hebei Province, China. xiaolanzh@hb2h.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Animals
MH  - Carbon Tetrachloride/toxicity
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Extracellular Matrix/metabolism
MH  - Fibrosis
MH  - Hepatic Stellate Cells/physiology
MH  - Hepatocytes/physiology
MH  - Humans
MH  - Liver/cytology/drug effects/*pathology
MH  - Liver Cirrhosis/blood/chemically induced/pathology/*therapy
MH  - Liver Function Tests
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Umbilical Cord/cytology
PMC - PMC5739922
OTO - NOTNLM
OT  - Collagen metabolism
OT  - Differentiation
OT  - Hepatocyte
OT  - Liver fibrosis/cirrhosis
OT  - Mesenchymal stem cells
COIS- Conflict-of-interest statement: We declare that there are no conflicts of 
      interest to disclose.
EDAT- 2018/01/02 06:00
MHDA- 2018/08/15 06:00
PMCR- 2017/12/14
CRDT- 2018/01/02 06:00
PHST- 2017/03/27 00:00 [received]
PHST- 2017/05/29 00:00 [revised]
PHST- 2017/08/09 00:00 [accepted]
PHST- 2018/01/02 06:00 [entrez]
PHST- 2018/01/02 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
PHST- 2017/12/14 00:00 [pmc-release]
AID - 10.3748/wjg.v23.i46.8152 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2017 Dec 14;23(46):8152-8168. doi: 
      10.3748/wjg.v23.i46.8152.