PMID- 29293082
OWN - NLM
STAT- MEDLINE
DCOM- 20180206
LR  - 20180206
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 67
IP  - 2
DP  - 2018 Feb
TI  - Actinobacillus pleuropneumoniae culture supernatant antiviral effect against 
      porcine reproductive and respiratory syndrome virus occurs prior to the viral 
      genome replication and transcription through actin depolymerization.
PG  - 249-264
LID - 10.1099/jmm.0.000659 [doi]
AB  - PURPOSE: Recently, the strong antiviral activity of an Actinobacillus 
      pleuropneumoniae (App) culture supernatant against porcine reproductive and 
      respiratory syndrome virus (PRRSV) was discovered. Following this finding, the 
      objective of the present study was to understand how the App culture supernatant 
      inhibits PRRSV replication in its natural targeted host cells, i.e. porcine 
      alveolar macrophages (PAMs). METHODOLOGY: Several assays were conducted with App 
      culture supernatant-treated PRRSV-infected cell lines, such as PAM, St-Jude 
      porcine lung and MARC-145 cells. RT-qPCR assays were used to determine the 
      expression levels of type I and II IFN mRNAs, viral genomic (gRNA) and 
      sub-genomic RNAs (sgRNAs). Proteomic, Western blot and immunofluorescence assays 
      were conducted to determine the involvement of actin filaments in the App culture 
      supernatant antiviral effect.Results/Key findings. Type I and II IFN mRNA 
      expressions were not upregulated by the App culture supernatant. Time courses of 
      gRNA and sgRNA expression levels demonstrated that the App culture supernatant 
      inhibits PRRSV infection before the first viral transcription cycle. Western blot 
      experiments confirmed an increase in the expression of cofilin (actin 
      cytoskeleton dynamics regulator) and immunofluorescence also demonstrated a 
      significant decrease of actin filaments in App culture supernatant-treated 
      PRRSV-infected PAM cells. App culture supernatant antiviral activity was also 
      demonstrated against other PRRSV strains of genotypes I and II. CONCLUSION: App 
      culture supernatant antiviral effect against PRRSV takes place early during PRRSV 
      infection. Results suggest that App culture supernatant antiviral effect may take 
      place via the activation of cofilin, which induces actin depolymerization and 
      subsequently, probably affects PRRSV endocytosis. Other experiments are needed to 
      fully validate this latest hypothesis.
FAU - Hernandez Reyes, Yenney
AU  - Hernandez Reyes Y
AD  - Centre de recherche en infectiologie porcine et avicole (CRIPA) et Groupe de 
      recherche sur les maladies infectieuses en production animale (GREMIP), Faculte 
      de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, J2S 2M2, 
      Canada.
FAU - Provost, Chantale
AU  - Provost C
AD  - Centre de recherche en infectiologie porcine et avicole (CRIPA) et Groupe de 
      recherche sur les maladies infectieuses en production animale (GREMIP), Faculte 
      de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, J2S 2M2, 
      Canada.
FAU - Traesel, Carolina Kist
AU  - Traesel CK
AD  - Centre de recherche en infectiologie porcine et avicole (CRIPA) et Groupe de 
      recherche sur les maladies infectieuses en production animale (GREMIP), Faculte 
      de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, J2S 2M2, 
      Canada.
FAU - Jacques, Mario
AU  - Jacques M
AD  - Centre de recherche en infectiologie porcine et avicole (CRIPA) et Groupe de 
      recherche sur les maladies infectieuses en production animale (GREMIP), Faculte 
      de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, J2S 2M2, 
      Canada.
FAU - Gagnon, Carl A
AU  - Gagnon CA
AD  - Centre de recherche en infectiologie porcine et avicole (CRIPA) et Groupe de 
      recherche sur les maladies infectieuses en production animale (GREMIP), Faculte 
      de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, J2S 2M2, 
      Canada.
LA  - eng
PT  - Journal Article
DEP - 20180102
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Actins)
RN  - 0 (Antiviral Agents)
RN  - 0 (Culture Media)
RN  - 0 (Interferon Type I)
SB  - IM
MH  - Actinobacillus pleuropneumoniae/*physiology
MH  - Actins/*metabolism
MH  - Animals
MH  - Antibiosis
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line
MH  - Culture Media/chemistry
MH  - Genome, Viral
MH  - Interferon Type I/genetics
MH  - Macrophages, Alveolar/drug effects/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Swine
MH  - Transcription, Genetic/drug effects
MH  - Virus Replication/*drug effects
OTO - NOTNLM
OT  - Actinobacillus pleuropneumoniae
OT  - actin
OT  - antiviral effect
OT  - cofilin
OT  - porcine alveolar macrophages
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2018/01/03 06:00
MHDA- 2018/02/07 06:00
CRDT- 2018/01/03 06:00
PHST- 2018/01/03 06:00 [pubmed]
PHST- 2018/02/07 06:00 [medline]
PHST- 2018/01/03 06:00 [entrez]
AID - 10.1099/jmm.0.000659 [doi]
PST - ppublish
SO  - J Med Microbiol. 2018 Feb;67(2):249-264. doi: 10.1099/jmm.0.000659. Epub 2018 Jan 
      2.