PMID- 29293689
OWN - NLM
STAT- MEDLINE
DCOM- 20180625
LR  - 20231213
IS  - 1553-7374 (Electronic)
IS  - 1553-7366 (Print)
IS  - 1553-7366 (Linking)
VI  - 14
IP  - 1
DP  - 2018 Jan
TI  - Tomato leaf curl Yunnan virus-encoded C4 induces cell division through enhancing 
      stability of Cyclin D 1.1 via impairing NbSKeta -mediated phosphorylation in 
      Nicotiana benthamiana.
PG  - e1006789
LID - 10.1371/journal.ppat.1006789 [doi]
LID - e1006789
AB  - The whitefly-transmitted geminiviruses induce severe developmental abnormalities 
      in plants. Geminivirus-encoded C4 protein functions as one of viral symptom 
      determinants that could induce abnormal cell division. However, the molecular 
      mechanism by which C4 contributes to cell division induction remains unclear. 
      Here we report that tomato leaf curl Yunnan virus (TLCYnV) C4 interacts with a 
      glycogen synthase kinase 3 (GSK3)/SHAGGY-like kinase, designed NbSKeta, in 
      Nicotiana benthamiana. Pro32, Asn34 and Thr35 of TLCYnV C4 are critical for its 
      interaction with NbSKeta and required for C4-induced typical symptoms. 
      Interestingly, TLCYnV C4 directs NbSKeta to the membrane and reduces the 
      nuclear-accumulation of NbSKeta. The relocalization of NbSKeta impairs 
      phosphorylation dependent degradation on its substrate-Cyclin D1.1 (NbCycD1;1), 
      thereby increasing the accumulation level of NbCycD1;1 and inducing the cell 
      division. Moreover, NbSKeta-RNAi, 35S::NbCycD1;1 transgenic N. benthamiana plants 
      have the similar phenotype as 35S::C4 transgenic N. benthamiana plants on 
      callus-like tissue formation resulted from abnormal cell division induction. 
      Thus, this study provides new insights into mechanism of how a viral protein 
      hijacks NbSKeta to induce abnormal cell division in plants.
FAU - Mei, Yuzhen
AU  - Mei Y
AD  - State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang 
      University, Hangzhou, Zhejiang, China.
FAU - Yang, Xiuling
AU  - Yang X
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
FAU - Huang, Changjun
AU  - Huang C
AD  - State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang 
      University, Hangzhou, Zhejiang, China.
AD  - Department of Biochemistry & Biophysics, Texas A&M University, College Station, 
      United States of America.
AD  - Institute for Plant Genomics and Biotechnology, Texas A&M University, College 
      Station, United States of America.
FAU - Zhang, Xiuren
AU  - Zhang X
AUID- ORCID: 0000-0001-8982-2999
AD  - Department of Biochemistry & Biophysics, Texas A&M University, College Station, 
      United States of America.
AD  - Institute for Plant Genomics and Biotechnology, Texas A&M University, College 
      Station, United States of America.
FAU - Zhou, Xueping
AU  - Zhou X
AUID- ORCID: 0000-0001-5311-7331
AD  - State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang 
      University, Hangzhou, Zhejiang, China.
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180102
PL  - United States
TA  - PLoS Pathog
JT  - PLoS pathogens
JID - 101238921
RN  - 0 (Luminescent Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - Tomato leaf curl virus
SB  - IM
MH  - Agrobacterium tumefaciens/physiology
MH  - Begomovirus/*metabolism/pathogenicity
MH  - Cell Division
MH  - Cyclin D1/chemistry/*metabolism
MH  - Gene Deletion
MH  - Glycogen Synthase Kinase 3/*metabolism
MH  - Luminescent Proteins/chemistry/genetics/metabolism
MH  - Phosphorylation
MH  - Phylogeny
MH  - Plant Leaves/genetics/metabolism/microbiology/ultrastructure
MH  - Plant Proteins/antagonists & inhibitors/chemistry/genetics/*metabolism
MH  - Plants, Genetically Modified/genetics/metabolism/microbiology/ultrastructure
MH  - Point Mutation
MH  - Proteasome Endopeptidase Complex/metabolism/ultrastructure
MH  - Protein Interaction Domains and Motifs
MH  - Protein Processing, Post-Translational
MH  - Protein Serine-Threonine Kinases/antagonists & 
      inhibitors/chemistry/genetics/*metabolism
MH  - Protein Stability
MH  - Protein Transport
MH  - Proteolysis
MH  - RNA Interference
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Nicotiana/genetics/*metabolism/microbiology/ultrastructure
MH  - Viral Proteins/chemistry/*metabolism
PMC - PMC5766254
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/01/03 06:00
MHDA- 2018/06/26 06:00
PMCR- 2018/01/02
CRDT- 2018/01/03 06:00
PHST- 2017/06/13 00:00 [received]
PHST- 2017/12/04 00:00 [accepted]
PHST- 2018/01/12 00:00 [revised]
PHST- 2018/01/03 06:00 [pubmed]
PHST- 2018/06/26 06:00 [medline]
PHST- 2018/01/03 06:00 [entrez]
PHST- 2018/01/02 00:00 [pmc-release]
AID - PPATHOGENS-D-17-01274 [pii]
AID - 10.1371/journal.ppat.1006789 [doi]
PST - epublish
SO  - PLoS Pathog. 2018 Jan 2;14(1):e1006789. doi: 10.1371/journal.ppat.1006789. 
      eCollection 2018 Jan.