PMID- 29298343
OWN - NLM
STAT- MEDLINE
DCOM- 20180215
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 1
DP  - 2018
TI  - EpCAM peptide-primed dendritic cell vaccination confers significant anti-tumor 
      immunity in hepatocellular carcinoma cells.
PG  - e0190638
LID - 10.1371/journal.pone.0190638 [doi]
LID - e0190638
AB  - Cancer stem-like cells (CSCs) may play a key role in tumor initiation, 
      self-renewal, differentiation, and resistance to current treatments. Dendritic 
      cells (DCs) play a vital role in host immune reactions as well as antigen 
      presentation. In this study, we explored the suitability of using CSC peptides as 
      antigen sources for DC vaccination against human breast cancer and hepatocellular 
      carcinoma (HCC) with the aim of achieving CSC targeting and enhancing anti-tumor 
      immunity. CD44 is used as a CSC marker for breast cancer and EpCAM is used as a 
      CSC marker for HCC. We selected CD44 and EpCAM peptides that bind to HLA-A2 
      molecules on the basis of their binding affinity, as determined by a peptide-T2 
      binding assay. Our data showed that CSCs express high levels of tumor-associated 
      antigens (TAAs) as well as major histocompatibility complex (MHC) molecules. 
      Pulsing DCs with CD44 and EpCAM peptides resulted in the efficient generation of 
      mature DCs (mDCs), thus enhancing T cell stimulation and generating potent 
      cytotoxic T lymphocytes (CTLs). The activation of CSC peptide-specific immune 
      responses by the DC vaccine in combination with standard chemotherapy may provide 
      better clinical outcomes in advanced carcinomas.
FAU - Choi, Yoo Jin
AU  - Choi YJ
AD  - Research Center, Dongnam Institute of Radiological & Medical Sciences, Busan, 
      Republic of Korea.
FAU - Park, Seong-Joon
AU  - Park SJ
AD  - Research Center, Dongnam Institute of Radiological & Medical Sciences, Busan, 
      Republic of Korea.
FAU - Park, You-Soo
AU  - Park YS
AD  - Research Center, Dongnam Institute of Radiological & Medical Sciences, Busan, 
      Republic of Korea.
FAU - Park, Hee Sung
AU  - Park HS
AD  - Research Center, Dongnam Institute of Radiological & Medical Sciences, Busan, 
      Republic of Korea.
FAU - Yang, Kwang Mo
AU  - Yang KM
AD  - Research Center, Dongnam Institute of Radiological & Medical Sciences, Busan, 
      Republic of Korea.
AD  - Department of Radiation Oncology, Dongnam Institute of Radiological & Medical 
      Sciences, Busan, Republic of Korea.
AD  - Department of Radiation Oncology, Korea Institute of Radiological & Medical 
      Sciences, Seoul, Republic of Korea.
FAU - Heo, Kyu
AU  - Heo K
AUID- ORCID: 0000-0001-8833-4731
AD  - Research Center, Dongnam Institute of Radiological & Medical Sciences, Busan, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180103
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cancer Vaccines)
RN  - 0 (EPCAM protein, human)
RN  - 0 (Epithelial Cell Adhesion Molecule)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Animals
MH  - Cancer Vaccines/immunology/*therapeutic use
MH  - Carcinoma, Hepatocellular/immunology/*therapy
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cell Adhesion Molecule/*administration & dosage/chemistry
MH  - Female
MH  - HLA-A2 Antigen/immunology
MH  - Heterografts
MH  - Humans
MH  - Hyaluronan Receptors/immunology
MH  - Liver Neoplasms/immunology/*therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peptide Fragments/*administration & dosage
PMC - PMC5752035
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2018/01/04 06:00
MHDA- 2018/02/16 06:00
PMCR- 2018/01/03
CRDT- 2018/01/04 06:00
PHST- 2017/05/25 00:00 [received]
PHST- 2017/12/18 00:00 [accepted]
PHST- 2018/01/04 06:00 [entrez]
PHST- 2018/01/04 06:00 [pubmed]
PHST- 2018/02/16 06:00 [medline]
PHST- 2018/01/03 00:00 [pmc-release]
AID - PONE-D-17-20159 [pii]
AID - 10.1371/journal.pone.0190638 [doi]
PST - epublish
SO  - PLoS One. 2018 Jan 3;13(1):e0190638. doi: 10.1371/journal.pone.0190638. 
      eCollection 2018.