PMID- 29299929
OWN - NLM
STAT- MEDLINE
DCOM- 20180913
LR  - 20180913
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 17
IP  - 2
DP  - 2018 Feb 2
TI  - Proteome of Bovine Mitochondria and Rod Outer Segment Disks: Commonalities and 
      Differences.
PG  - 918-925
LID - 10.1021/acs.jproteome.7b00741 [doi]
AB  - The retinal rod outer segment (OS) is a stack of disks surrounded by the plasma 
      membrane, housing proteins related to phototransduction, as well as mitochondrial 
      proteins involved in oxidative phosphorylation (OxPhos). This prompted us to 
      compare the proteome of bovine OS disks and mitochondria to assess the 
      significant top gene signatures of each sample. The two proteomes, obtained by 
      LTQ-Orbitrap Velos mass spectrometry, were compared by statistical analyses. In 
      total, 4139 proteins were identified, 2045 of which overlapping in the two sets. 
      Nonhierarchical Spearman's correlogram revealed that the groups were clearly 
      discriminated. Partial least square discriminant plus support vector machine 
      analysis identified the major discriminative proteins, implied in 
      phototransduction and lipid metabolism, respectively. Gene Ontology analysis 
      identified top gene signatures of the disk proteome, enriched in vesiculation, 
      glycolysis, and OxPhos proteins. The tricarboxylic acid cycle and the electron 
      transport proteins were similarly enriched in the two samples, but the latter was 
      up regulated in disks. Data suggest that the mitochondrial OxPhos proteins may 
      represent a true OS proteome component, outside the mitochondrion. This knowledge 
      may help the scientific community in the further studies of retinal physiology 
      and pathology.
FAU - Bruschi, Maurizio
AU  - Bruschi M
FAU - Petretto, Andrea
AU  - Petretto A
FAU - Caicci, Federico
AU  - Caicci F
AD  - Department of Biology, Universita di Padova , 35121 Padova, Italy.
FAU - Bartolucci, Martina
AU  - Bartolucci M
FAU - Calzia, Daniela
AU  - Calzia D
AD  - Dipartimento di Farmacia-DIFAR, Universita di Genova , 16132 Genoa, Italy.
FAU - Santucci, Laura
AU  - Santucci L
FAU - Manni, Lucia
AU  - Manni L
AD  - Department of Biology, Universita di Padova , 35121 Padova, Italy.
FAU - Ramenghi, Luca A
AU  - Ramenghi LA
FAU - Ghiggeri, GianMarco
AU  - Ghiggeri G
FAU - Traverso, Carlo E
AU  - Traverso CE
AD  - Clinica Oculistica, (Di.N.O.G.M.I.) Universita Department of Intensive Care di 
      Genova, IRCCS Azienda Ospedaliera Universitaria San Martino-IST , 16132 Genoa, 
      Italy.
FAU - Candiano, Giovanni
AU  - Candiano G
FAU - Panfoli, Isabella
AU  - Panfoli I
AUID- ORCID: 0000-0002-6261-1128
AD  - Dipartimento di Farmacia-DIFAR, Universita di Genova , 16132 Genoa, Italy.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180118
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Eye Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chromatography, Liquid
MH  - Citric Acid Cycle/genetics
MH  - Eye Proteins/genetics/*isolation & purification/metabolism
MH  - Gene Ontology
MH  - Glycolysis/genetics
MH  - Least-Squares Analysis
MH  - Light Signal Transduction
MH  - Lipid Metabolism/genetics
MH  - Microscopy, Electron, Transmission
MH  - Mitochondria/*genetics/metabolism/ultrastructure
MH  - Mitochondrial Proteins/genetics/*isolation & purification/metabolism
MH  - Molecular Sequence Annotation
MH  - Oxidative Phosphorylation
MH  - Proteome/genetics/*isolation & purification/metabolism
MH  - Rod Cell Outer Segment/*metabolism/ultrastructure
MH  - Support Vector Machine
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - ATP synthase
OT  - disks
OT  - electron transfer chain
OT  - mass spectrometry
OT  - mitochondria
OT  - oxidative phosphorylation
OT  - rod outer segment
OT  - transmission electron microscopy
EDAT- 2018/01/05 06:00
MHDA- 2018/09/14 06:00
CRDT- 2018/01/05 06:00
PHST- 2018/01/05 06:00 [pubmed]
PHST- 2018/09/14 06:00 [medline]
PHST- 2018/01/05 06:00 [entrez]
AID - 10.1021/acs.jproteome.7b00741 [doi]
PST - ppublish
SO  - J Proteome Res. 2018 Feb 2;17(2):918-925. doi: 10.1021/acs.jproteome.7b00741. 
      Epub 2018 Jan 18.