PMID- 29305975
OWN - NLM
STAT- MEDLINE
DCOM- 20180202
LR  - 20180202
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 646
DP  - 2018 Mar 10
TI  - Identification and expression analysis of microRNAs in medaka gonads.
PG  - 210-216
LID - S0378-1119(17)31131-9 [pii]
LID - 10.1016/j.gene.2017.12.062 [doi]
AB  - Gonad development is a highly regulated, coordinated biological process and 
      increasing evidences have indicated that microRNA (miRNA) may be involved in this 
      dynamic program. Medaka (Oryzias latipes) is a good model for reproductive 
      research as it has distinct sex determining genes, however, research in gonadal 
      miRNAs is lacked. In this study, two small RNA libraries from the ovaries and 
      testes were constructed and sequenced. A total of 285 conserved and 388 novel 
      miRNAs were obtained, among which 142 mature miRNAs were significantly (> 
      two-fold change) up or down regulated in the testis compared to the ovary. 
      Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis 
      showed that miR-430c, miR-26a and miR-202-5p were expressed in a gonad-specific 
      or sex-biased pattern. Fluorescence in situ hybridization (FISH) indicated that 
      miR-202-5p was present throughout spermatogenesis and was only detected at the 
      early stages of oogenesis, this sex biased expression pattern suggested that 
      miR-202-5p might be a crucial candidate in male differentiation and development. 
      Our study provides the repertoire, a comprehensive annotation of miRNAs from 
      gonads and a reference for functional studies of miRNAs in medaka.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Qiu, Weiwei
AU  - Qiu W
AD  - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture 
      Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration 
      and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 
      Ocean University, Shanghai 201306, China; National Demonstration Center for 
      Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 
      201306, China.
FAU - Zhu, Yefei
AU  - Zhu Y
AD  - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture 
      Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration 
      and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 
      Ocean University, Shanghai 201306, China; National Demonstration Center for 
      Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 
      201306, China.
FAU - Wu, Yun
AU  - Wu Y
AD  - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture 
      Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration 
      and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 
      Ocean University, Shanghai 201306, China; National Demonstration Center for 
      Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 
      201306, China.
FAU - Yuan, Cancan
AU  - Yuan C
AD  - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture 
      Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration 
      and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 
      Ocean University, Shanghai 201306, China; National Demonstration Center for 
      Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 
      201306, China.
FAU - Chen, Kerang
AU  - Chen K
AD  - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture 
      Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration 
      and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 
      Ocean University, Shanghai 201306, China; National Demonstration Center for 
      Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 
      201306, China.
FAU - Li, Mingyou
AU  - Li M
AD  - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture 
      Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration 
      and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 
      Ocean University, Shanghai 201306, China; National Demonstration Center for 
      Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 
      201306, China. Electronic address: myli@shou.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180103
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation, Developmental
MH  - Gene Library
MH  - Gonads/chemistry/*growth & development
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Oogenesis
MH  - Organ Specificity
MH  - Oryzias/genetics/*physiology
MH  - Sexism
MH  - Spermatogenesis
OTO - NOTNLM
OT  - Gonad
OT  - Medaka
OT  - MicroRNA
OT  - mir-202-5p
EDAT- 2018/01/07 06:00
MHDA- 2018/02/03 06:00
CRDT- 2018/01/07 06:00
PHST- 2017/09/14 00:00 [received]
PHST- 2017/12/08 00:00 [revised]
PHST- 2017/12/29 00:00 [accepted]
PHST- 2018/01/07 06:00 [pubmed]
PHST- 2018/02/03 06:00 [medline]
PHST- 2018/01/07 06:00 [entrez]
AID - S0378-1119(17)31131-9 [pii]
AID - 10.1016/j.gene.2017.12.062 [doi]
PST - ppublish
SO  - Gene. 2018 Mar 10;646:210-216. doi: 10.1016/j.gene.2017.12.062. Epub 2018 Jan 3.