PMID- 29307520
OWN - NLM
STAT- MEDLINE
DCOM- 20181231
LR  - 20190501
IS  - 1532-8600 (Electronic)
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 82
DP  - 2018 May
TI  - Skeletal muscle ceramides and daily fat oxidation in obesity and diabetes.
PG  - 118-123
LID - S0026-0495(17)30359-1 [pii]
LID - 10.1016/j.metabol.2017.12.012 [doi]
AB  - BACKGROUND/OBJECTIVES: Ectopic accumulation of lipids in skeletal muscle and the 
      formation of deleterious lipid intermediates is thought to contribute to the 
      development of insulin resistance and type 2 diabetes mellitus (T2DM). Similarly, 
      impaired fat oxidation (metabolic inflexibility) are predictors of weight gain 
      and the development of T2DM; however, no study has investigated the relation 
      between muscle ceramide accumulation and 24-hour macronutrient oxidation. The 
      purpose of this study was to retrospectively explore the relationships between 
      whole body fat oxidation and skeletal muscle ceramide accumulation in obese 
      non-diabetic individuals (ND) and in people with obesity and T2DM. METHODS: Daily 
      substrate oxidation was measured in a respiratory chamber and skeletal muscle 
      ceramides were measured using liquid chromatographyelectrospray ionization 
      tandem-mass spectrometry. RESULTS: After adjusting for sex, age, and BMI, no 
      differences existed between the groups for fat oxidation or 24-h RQ. However, 
      ceramides C18:1, C:20, C22, C24 and C24:1 were significantly higher in people 
      with T2DM compared to ND whereas no differences existed for C16 and C18. Despite 
      low amounts of muscle ceramides, fat oxidation rates were positively associated 
      with ceramide species concentration in ND only. Our data suggests that ceramides 
      do not interfere with whole-body fat oxidation in ND individuals whereas a 
      persistent lipid oversupply results in excessive ceramide muscle accumulation in 
      people with T2DM.
CI  - Copyright (c) 2018. Published by Elsevier Inc.
FAU - Broskey, Nicholas T
AU  - Broskey NT
AD  - Pennington Biomedical Research Center, Baton Rouge, LA 70808, United States.
FAU - Obanda, Diana N
AU  - Obanda DN
AD  - Pennington Biomedical Research Center, Baton Rouge, LA 70808, United States.
FAU - Burton, Jeffrey H
AU  - Burton JH
AD  - Pennington Biomedical Research Center, Baton Rouge, LA 70808, United States.
FAU - Cefalu, William T
AU  - Cefalu WT
AD  - Pennington Biomedical Research Center, Baton Rouge, LA 70808, United States.
FAU - Ravussin, Eric
AU  - Ravussin E
AD  - Pennington Biomedical Research Center, Baton Rouge, LA 70808, United States. 
      Electronic address: eric.ravussin@pbrc.edu.
LA  - eng
SI  - ClinicalTrials.gov/NCT00398853
SI  - ClinicalTrials.gov/NCT01672632
SI  - ClinicalTrials.gov/NCT00936130
GR  - R55 DK060126/DK/NIDDK NIH HHS/United States
GR  - P30 DK072476/DK/NIDDK NIH HHS/United States
GR  - U54 GM104940/GM/NIGMS NIH HHS/United States
GR  - R01 DK060412/DK/NIDDK NIH HHS/United States
GR  - R01 DK060126/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180104
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Ceramides)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Body Mass Index
MH  - Ceramides/*metabolism
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Energy Metabolism/*physiology
MH  - Female
MH  - Humans
MH  - Insulin Resistance/physiology
MH  - Lipid Metabolism/physiology
MH  - Male
MH  - Middle Aged
MH  - Muscle, Skeletal/*metabolism
MH  - Obesity/*metabolism
MH  - Oxidation-Reduction
MH  - Young Adult
PMC - PMC5930033
MID - NIHMS940025
OTO - NOTNLM
OT  - Energy expenditure
OT  - Lipotoxicity
OT  - Type 2 diabetes
COIS- Conflicts of Interest The authors have no conflicts of interest to declare.
EDAT- 2018/01/09 06:00
MHDA- 2019/01/01 06:00
PMCR- 2019/05/01
CRDT- 2018/01/09 06:00
PHST- 2017/09/21 00:00 [received]
PHST- 2017/11/15 00:00 [revised]
PHST- 2017/12/27 00:00 [accepted]
PHST- 2018/01/09 06:00 [pubmed]
PHST- 2019/01/01 06:00 [medline]
PHST- 2018/01/09 06:00 [entrez]
PHST- 2019/05/01 00:00 [pmc-release]
AID - S0026-0495(17)30359-1 [pii]
AID - 10.1016/j.metabol.2017.12.012 [doi]
PST - ppublish
SO  - Metabolism. 2018 May;82:118-123. doi: 10.1016/j.metabol.2017.12.012. Epub 2018 
      Jan 4.