PMID- 29307597
OWN - NLM
STAT- MEDLINE
DCOM- 20180420
LR  - 20180420
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 517
DP  - 2018 Apr
TI  - Intercellular transfer of mitochondria rescues virus-induced cell death but 
      facilitates cell-to-cell spreading of porcine reproductive and respiratory 
      syndrome virus.
PG  - 122-134
LID - S0042-6822(17)30423-3 [pii]
LID - 10.1016/j.virol.2017.12.018 [doi]
AB  - Our recent study showed that intercellular tunneling nanotubes (TNTs) serve as an 
      alternative pathway for cell-to-cell spreading of infectious materials of porcine 
      reproductive and respiratory syndrome virus (PRRSV). In this study, we found that 
      PRRSV infection could induce the formation of TNTs between infected and 
      uninfected cells. Co-culturing PRRSV-infected cells with uninfected cells, 
      including porcine umbilical cord stem cells, rescued PRRSV-induced cell death. 
      Mitochondrion, an important regulator of cell survival/death, was observed 
      transferring from uninfected to PRRSV-infected cells. Importantly, impaired 
      formation of nanotube or defective mitochondrion was unable to rescue infected 
      cells from apoptosis/necrosis. Certain PRRSV proteins were detected to associate 
      with mitochondria and transport from infected to uninfected cells through TNTs. 
      Our results suggest that TNTs-transfer of functional mitochondria rescued 
      PRRSV-infected cells from apoptosis/necrosis in the early stage of infection. On 
      the other hand, mitochondria could be utilized as a vehicle to transport viral 
      materials for spreading the infection.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Guo, Rui
AU  - Guo R
AD  - Department of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS 66506, United States.
FAU - Davis, Duane
AU  - Davis D
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan, 
      KS 66506, United States.
FAU - Fang, Ying
AU  - Fang Y
AD  - Department of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS 66506, United States. Electronic address: yfang@vet.k-state.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180104
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
SB  - IM
MH  - Animals
MH  - Cell Death
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Intercellular Junctions
MH  - Macrophages, Alveolar/physiology/virology
MH  - Mitochondria/*physiology
MH  - Nanotubes
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
OTO - NOTNLM
OT  - Cell death
OT  - Intercellular nanotube
OT  - Mitochondria
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
EDAT- 2018/01/09 06:00
MHDA- 2018/04/21 06:00
CRDT- 2018/01/09 06:00
PHST- 2017/09/29 00:00 [received]
PHST- 2017/12/14 00:00 [revised]
PHST- 2017/12/15 00:00 [accepted]
PHST- 2018/01/09 06:00 [pubmed]
PHST- 2018/04/21 06:00 [medline]
PHST- 2018/01/09 06:00 [entrez]
AID - S0042-6822(17)30423-3 [pii]
AID - 10.1016/j.virol.2017.12.018 [doi]
PST - ppublish
SO  - Virology. 2018 Apr;517:122-134. doi: 10.1016/j.virol.2017.12.018. Epub 2018 Jan 
      4.