PMID- 29310789
OWN - NLM
STAT- MEDLINE
DCOM- 20181114
LR  - 20210109
IS  - 0091-679X (Print)
IS  - 0091-679X (Linking)
VI  - 143
DP  - 2018
TI  - Investigating cell-type specific functions of tenascin-C.
PG  - 401-428
LID - S0091-679X(17)30144-9 [pii]
LID - 10.1016/bs.mcb.2017.08.023 [doi]
AB  - The extracellular matrix molecule tenascin-C (TNC) has received a lot of 
      attention since its discovery 30 years ago because of its multiple roles in 
      tissue repair, and in pathologies such as chronic inflammation, fibrosis, and 
      cancer. Mouse models with high or no TNC expression have enabled the validation 
      of key roles for TNC in immunity and angiogenesis. In parallel, many approaches 
      including primary cell or organ cultures have shed light on the cellular and 
      molecular mechanisms by which TNC exerts its multiple actions in vivo. Here, we 
      will describe assays that investigate its antiadhesive properties and that 
      measure the effect of TNC on the actin cytoskeleton, cell survival, 
      proliferation, and migration. We will also describe assays to assess the impact 
      of TNC on endothelial and immune cells in cell and organ culture, and to compare 
      the responses of fibroblasts from normal and diseased tissues.
CI  - (c) 2018 Elsevier Inc. All rights reserved.
FAU - Murdamoothoo, Devadarssen
AU  - Murdamoothoo D
AD  - Inserm U1109, The Microenvironmental Niche in Tumorigenesis and Targeted Therapy 
      (MNT3) Team, Strasbourg, France; Universite de Strasbourg, LabEx Medalis, 
      Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
FAU - Schwenzer, Anja
AU  - Schwenzer A
AD  - The Kennedy Institute of Rheumatology, University of Oxford, Oxford, United 
      Kingdom.
FAU - Kant, Jessica
AU  - Kant J
AD  - Inserm U1109, The Microenvironmental Niche in Tumorigenesis and Targeted Therapy 
      (MNT3) Team, Strasbourg, France; Universite de Strasbourg, LabEx Medalis, 
      Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
FAU - Rupp, Tristan
AU  - Rupp T
AD  - Inserm U1109, The Microenvironmental Niche in Tumorigenesis and Targeted Therapy 
      (MNT3) Team, Strasbourg, France; Universite de Strasbourg, LabEx Medalis, 
      Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
FAU - Marzeda, Anna
AU  - Marzeda A
AD  - The Kennedy Institute of Rheumatology, University of Oxford, Oxford, United 
      Kingdom.
FAU - Midwood, Kim
AU  - Midwood K
AD  - The Kennedy Institute of Rheumatology, University of Oxford, Oxford, United 
      Kingdom. Electronic address: kim.midwood@kennedy.ox.ac.uk.
FAU - Orend, Gertraud
AU  - Orend G
AD  - Inserm U1109, The Microenvironmental Niche in Tumorigenesis and Targeted Therapy 
      (MNT3) Team, Strasbourg, France; Universite de Strasbourg, LabEx Medalis, 
      Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France. 
      Electronic address: gertraud.orend@inserm.fr.
LA  - eng
GR  - 20003/VAC_/Versus Arthritis/United Kingdom
GR  - ARC_/Arthritis Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171201
PL  - United States
TA  - Methods Cell Biol
JT  - Methods in cell biology
JID - 0373334
RN  - 0 (Tenascin)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Animals
MH  - Biological Assay/instrumentation/*methods
MH  - Cell Culture Techniques/instrumentation/methods
MH  - Cell Movement/physiology
MH  - Cell Proliferation/physiology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Chickens
MH  - Chorioallantoic Membrane
MH  - Extracellular Matrix/*metabolism
MH  - Fibroblasts/*metabolism
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Mice
MH  - Models, Animal
MH  - Molecular Imaging/*methods
MH  - Neovascularization, Physiologic/physiology
MH  - Tenascin/analysis/*physiology
OTO - NOTNLM
OT  - Actin cytoskeleton
OT  - Cell adhesion
OT  - Chicken chorioallantoic membrane assay
OT  - Endothelial cell
OT  - Immune cell activation
OT  - Immunofluorescence
OT  - Matrigel tubulogenesis assay
OT  - Proliferation assay
OT  - Survival assay
OT  - Synovial fibroblast
OT  - Tenascin-C
OT  - Wound healing assay
OT  - extracellular matrix
EDAT- 2018/01/10 06:00
MHDA- 2018/11/15 06:00
CRDT- 2018/01/10 06:00
PHST- 2018/01/10 06:00 [entrez]
PHST- 2018/01/10 06:00 [pubmed]
PHST- 2018/11/15 06:00 [medline]
AID - S0091-679X(17)30144-9 [pii]
AID - 10.1016/bs.mcb.2017.08.023 [doi]
PST - ppublish
SO  - Methods Cell Biol. 2018;143:401-428. doi: 10.1016/bs.mcb.2017.08.023. Epub 2017 
      Dec 1.