PMID- 29310813
OWN - NLM
STAT- MEDLINE
DCOM- 20181224
LR  - 20191210
IS  - 2410-8650 (Electronic)
IS  - 1607-551X (Linking)
VI  - 34
IP  - 1
DP  - 2018 Jan
TI  - Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat 
      model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway.
PG  - 22-33
LID - S1607-551X(17)30280-2 [pii]
LID - 10.1016/j.kjms.2017.09.004 [doi]
AB  - We aim to investigate the effects of sevoflurane on the ATPase activity of the 
      hippocampal neurons in rats with cerebral ischemia-reperfusion injury (IRI) via 
      the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) signaling 
      pathway. Sixty rats were assigned into the normal, model and sevoflurane groups 
      (n = 20, the latter two groups were established as focal cerebral IRI models). 
      The ATPase activity was detected using an ultramicro Na (+)-K (+)-ATP enzyme kit. 
      Immunohistochemical staining was used to detect the positive protein expression 
      of cAMP and PKA. The hippocampal neurons were assigned to the normal, IRI, 
      IRI + sevoflurane, IRI + forskolin, IRI + H89 and IRI + sevoflurane + H89 groups. 
      qRT-PCR and Western blotting were performed for the expressions of cAMP, PKA, 
      cAMP-responsive element-binding protein (CREB) and brain derived neurotrophic 
      factor (BDNF). The normal and sevoflurane groups exhibited a greater positive 
      protein expression of cAMP and PKA than the model group. Compared with the normal 
      group, the expressions of cAMP, PKA, CREB and BDNF all reduced in the IRI, model 
      and IRI + H89 groups. The sevoflurane group showed higher cAMP, PKA, CREB and 
      BDNF expressions than the model group. Compared with the IRI group, ATPase 
      activity and expressions of cAMP, PKA, CREB and BDNF all increased in the normal, 
      IRI + sevoflurane and IRI + forskolin groups but decreased in the IRI + H89 
      group. It suggests that sevoflurane could enhance ATPase activity in hippocampal 
      neurons of cerebral IRI rats through activating cAMP-PKA signaling pathway.
CI  - Copyright (c) 2017. Published by Elsevier Taiwan.
FAU - Liu, Tie-Jun
AU  - Liu TJ
AD  - Department of Anesthesia, The Affiliated Hospital of North China University of 
      Science and Technology, Tangshan, PR China.
FAU - Zhang, Jin-Cun
AU  - Zhang JC
AD  - Department of Urology Surgery, The Affiliated Hospital of North China University 
      of Science and Technology, Tangshan, PR China.
FAU - Gao, Xiao-Zeng
AU  - Gao XZ
AD  - Department of Anesthesia, The Affiliated Hospital of North China University of 
      Science and Technology, Tangshan, PR China.
FAU - Tan, Zhi-Bin
AU  - Tan ZB
AD  - Department of Anesthesia, The Affiliated Hospital of North China University of 
      Science and Technology, Tangshan, PR China.
FAU - Wang, Jian-Jun
AU  - Wang JJ
AD  - Department of Critical Care Medicine, The Affiliated Hospital of North China 
      University of Science and Technology, Tangshan, PR China.
FAU - Zhang, Pan-Pan
AU  - Zhang PP
AD  - Department of Respiratory Medicine, The Affiliated Hospital of North China 
      University of Science and Technology, Tangshan, PR China.
FAU - Cheng, Ai-Bin
AU  - Cheng AB
AD  - Department of Critical Care Medicine, The Affiliated Hospital of North China 
      University of Science and Technology, Tangshan, PR China.
FAU - Zhang, Shu-Bo
AU  - Zhang SB
AD  - Department of Anesthesia, The Affiliated Hospital of North China University of 
      Science and Technology, Tangshan, PR China. Electronic address: 
      shubozsb17@163.com.
LA  - eng
PT  - Journal Article
DEP - 20171114
PL  - China (Republic : 1949- )
TA  - Kaohsiung J Med Sci
JT  - The Kaohsiung journal of medical sciences
JID - 100960562
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Isoquinolines)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Sulfonamides)
RN  - 1F7A44V6OU (Colforsin)
RN  - 38LVP0K73A (Sevoflurane)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (Crebbp protein, rat)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
RN  - M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - CREB-Binding Protein/genetics/metabolism
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/genetics/metabolism
MH  - Disease Models, Animal
MH  - Gene Expression Regulation
MH  - Germ-Free Life
MH  - Hippocampus/*drug effects/enzymology/pathology
MH  - Humans
MH  - Infarction, Middle Cerebral Artery/surgery
MH  - Isoquinolines/pharmacology
MH  - Male
MH  - Neurons/*drug effects/enzymology/pathology
MH  - Neuroprotective Agents/*pharmacology
MH  - Platelet Aggregation Inhibitors/pharmacology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*drug therapy/enzymology/genetics/pathology
MH  - Sevoflurane/*pharmacology
MH  - Sodium-Potassium-Exchanging ATPase/*genetics/metabolism
MH  - Sulfonamides/pharmacology
OTO - NOTNLM
OT  - ATPase activity
OT  - Cerebral ischemia-reperfusion injury
OT  - Hippocampal neuron
OT  - Sevoflurane
OT  - cAMP-PKA signaling pathway
EDAT- 2018/01/10 06:00
MHDA- 2018/12/26 06:00
CRDT- 2018/01/10 06:00
PHST- 2017/04/19 00:00 [received]
PHST- 2017/09/05 00:00 [revised]
PHST- 2017/09/13 00:00 [accepted]
PHST- 2018/01/10 06:00 [entrez]
PHST- 2018/01/10 06:00 [pubmed]
PHST- 2018/12/26 06:00 [medline]
AID - S1607-551X(17)30280-2 [pii]
AID - 10.1016/j.kjms.2017.09.004 [doi]
PST - ppublish
SO  - Kaohsiung J Med Sci. 2018 Jan;34(1):22-33. doi: 10.1016/j.kjms.2017.09.004. Epub 
      2017 Nov 14.