PMID- 29312500
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 9
IP  - 12
DP  - 2017
TI  - Endothelial MMP-9 drives the inflammatory response in abdominal aortic aneurysm 
      (AAA).
PG  - 5485-5495
AB  - Progression of abdominal aortic aneurysm (AAA) is typified by chronic 
      inflammation and extracellular matrix (ECM) degradation of the aortic wall. 
      Vascular inflammation involves complex interactions among inflammatory cells, 
      endothelial cells (ECs), vascular smooth muscle cells (vSMCs), and ECM. Although 
      vascular endothelium and medial neoangiogenesis play a key role in AAA, the 
      molecular mechanisms underlying their involvement are only partially understood. 
      In AAA biopsies, we found increased MMP-9, IL-6, and monocyte chemoattractant 
      protein-1 (MCP-1), which correlated with massive medial neo-angiogenesis (C4d 
      positive staining). In this study, we developed an in vitro model in order to 
      characterize the role of endothelial matrix metalloproteinase-9 (e-MMP-9) as a 
      potential trigger of medial disruption and in the inflammatory response bridging 
      between ECs and vSMC. Lentiviral-mediated silencing of e-MMP-9 through RNA 
      interference inhibited TNF-alpha-mediated activation of NF-kappaB in EA.hy926 human 
      endothelial cells. In addition, EA.hy926 cells void of MMP-9 failed to migrate in 
      a 3D matrix. Moreover, silenced EA.hy926 affected vSMC behavior in terms of 
      matrix remodeling. In fact, also MMP-9 in vSMC resulted inhibited when 
      endothelial MMP-9 was suppressed.
FAU - Ramella, Martina
AU  - Ramella M
AD  - Department of Health Sciences, Universita del Piemonte Orientale (UPO)Novara, 
      Italy.
FAU - Boccafoschi, Francesca
AU  - Boccafoschi F
AD  - Department of Health Sciences, Universita del Piemonte Orientale (UPO)Novara, 
      Italy.
FAU - Bellofatto, Kevin
AU  - Bellofatto K
AD  - Department of Health Sciences, Universita del Piemonte Orientale (UPO)Novara, 
      Italy.
FAU - Follenzi, Antonia
AU  - Follenzi A
AD  - Department of Health Sciences, Universita del Piemonte Orientale (UPO)Novara, 
      Italy.
FAU - Fusaro, Luca
AU  - Fusaro L
AD  - Department of Health Sciences, Universita del Piemonte Orientale (UPO)Novara, 
      Italy.
FAU - Boldorini, Renzo
AU  - Boldorini R
AD  - Department of Health Sciences, Universita del Piemonte Orientale (UPO)Novara, 
      Italy.
FAU - Casella, Francesco
AU  - Casella F
AD  - Department of Vascular Surgery, Ospedale Maggiore della CaritaNovara, Italy.
FAU - Porta, Carla
AU  - Porta C
AD  - Department of Vascular Surgery, Ospedale Maggiore della CaritaNovara, Italy.
FAU - Settembrini, Piergiorgio
AU  - Settembrini P
AD  - Division of Vascular Surgery, Universita di MilanoMilan, Italy.
FAU - Cannas, Mario
AU  - Cannas M
AD  - Department of Health Sciences, Universita del Piemonte Orientale (UPO)Novara, 
      Italy.
LA  - eng
PT  - Journal Article
DEP - 20171215
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5752898
OTO - NOTNLM
OT  - Abdominal aortic aneurysm
OT  - MMP-9 silencing
OT  - endothelium dysfunction
OT  - inflammatory response
OT  - vascular remodeling
COIS- None.
EDAT- 2018/01/10 06:00
MHDA- 2018/01/10 06:01
PMCR- 2017/12/15
CRDT- 2018/01/10 06:00
PHST- 2017/06/21 00:00 [received]
PHST- 2017/10/15 00:00 [accepted]
PHST- 2018/01/10 06:00 [entrez]
PHST- 2018/01/10 06:00 [pubmed]
PHST- 2018/01/10 06:01 [medline]
PHST- 2017/12/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2017 Dec 15;9(12):5485-5495. eCollection 2017.