PMID- 29313211
OWN - NLM
STAT- MEDLINE
DCOM- 20180830
LR  - 20210109
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 43
IP  - 3
DP  - 2018 Mar
TI  - Up-Regulation of CX3CL1 via STAT3 Contributes to SMIR-Induced Chronic 
      Postsurgical Pain.
PG  - 556-565
LID - 10.1007/s11064-017-2449-8 [doi]
AB  - Chronic postsurgical pain (CPSP) often occurs after surgery and has a strong 
      impact on patients' daily lives. However, the underlying mechanism of CPSP 
      remains unknown. Here, we used a skin/muscle incision and retraction (SMIR) model 
      to investigate the role of CX3CL1 in SMIR-induced pain and its underlying 
      mechanism. We found that up-regulation of CX3CL1 in the spinal dorsal horn 
      contributed to SMIR-induced mechanical allodynia. The use of a 
      CX3CL1-neutralizing antibody to block CX3CL1 attenuated mechanical allodynia 
      induced by SMIR surgery. We also found that phospho-STAT3 co-localizes with 
      CX3CL1 in spinal neurons after SMIR surgery and that this contributes to 
      SMIR-induced mechanical allodynia. Intrathecal administration of the STAT3 
      inhibitor S3I-201 suppressed up-regulation of CX3CL1 at both the protein and mRNA 
      levels after SMIR surgery. Chromatin immunoprecipitation further demonstrated 
      that SMIR promotes the recruitment of STAT3 to the cx3cl1 gene promoter 
      (- 1032/- 1022). These findings suggest that activation of STAT3 after SMIR 
      mediates the up-regulation of CX3CL1, leading to mechanical allodynia, and that 
      this upregulation may partly be due to the enhanced recruitment of STAT3 to the 
      cx3cl1 gene promoter after SMIR.
FAU - Shen, Yijia
AU  - Shen Y
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Li, Dai
AU  - Li D
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Li, Bo
AU  - Li B
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Xi, Peng
AU  - Xi P
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Zhang, Yun
AU  - Zhang Y
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Jiang, Youshui
AU  - Jiang Y
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Xu, Yehao
AU  - Xu Y
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Chen, Hui
AU  - Chen H
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China. chenhui_md@163.com.
FAU - Xiong, Yuanchang
AU  - Xiong Y
AD  - Department of Anesthesiology, The First Affiliated Hospital of the Second 
      Military Medical University, 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China. proychxiong@163.com.
LA  - eng
GR  - 81600955/National Natural Science Foundation of China/
GR  - 17ZR1438200/Natural Science Foundation of Shanghai/
PT  - Journal Article
DEP - 20180108
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Cx3cl1 protein, rat)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, rat)
SB  - IM
MH  - Animals
MH  - Chemokine CX3CL1/*metabolism
MH  - Chronic Pain/*metabolism
MH  - Disease Models, Animal
MH  - Hyperalgesia/metabolism
MH  - Male
MH  - Muscles/surgery
MH  - Pain Measurement/methods
MH  - Pain, Postoperative/*metabolism
MH  - Rats, Sprague-Dawley
MH  - STAT3 Transcription Factor/*metabolism
MH  - Skin/injuries
MH  - Transcriptional Activation/physiology
MH  - *Up-Regulation
OTO - NOTNLM
OT  - CX3CL1
OT  - Chronic postsurgical pain
OT  - P-STAT3
OT  - Skin/muscle incision and retraction
EDAT- 2018/01/10 06:00
MHDA- 2018/08/31 06:00
CRDT- 2018/01/10 06:00
PHST- 2017/09/11 00:00 [received]
PHST- 2017/12/05 00:00 [accepted]
PHST- 2017/11/29 00:00 [revised]
PHST- 2018/01/10 06:00 [pubmed]
PHST- 2018/08/31 06:00 [medline]
PHST- 2018/01/10 06:00 [entrez]
AID - 10.1007/s11064-017-2449-8 [pii]
AID - 10.1007/s11064-017-2449-8 [doi]
PST - ppublish
SO  - Neurochem Res. 2018 Mar;43(3):556-565. doi: 10.1007/s11064-017-2449-8. Epub 2018 
      Jan 8.