PMID- 29316077
OWN - NLM
STAT- MEDLINE
DCOM- 20180312
LR  - 20210109
IS  - 1349-7006 (Electronic)
IS  - 1347-9032 (Print)
IS  - 1347-9032 (Linking)
VI  - 109
IP  - 3
DP  - 2018 Mar
TI  - Cellular stress induces cancer stem-like cells through expression of DNAJB8 by 
      activation of heat shock factor 1.
PG  - 741-750
LID - 10.1111/cas.13501 [doi]
AB  - In a previous study, we found that DNAJB8, a heat shock protein (HSP) 40 family 
      member is expressed in kidney cancer stem-like cells (CSC)/cancer-initiating 
      cells (CIC) and that it has a role in the maintenance of kidney CSC/CIC. Heat 
      shock factor (HSF) 1 is a key transcription factor for responses to stress 
      including heat shock, and it induces HSP family expression through activation by 
      phosphorylation. In the present study, we therefore examined whether heat shock 
      (HS) induces CSC/CIC. We treated the human kidney cancer cell line ACHN with HS, 
      and found that HS increased side population (SP) cells. Western blot analysis and 
      qRT-PCR showed that HS increased the expression of DNAJB8 and SOX2. Gene 
      knockdown experiments using siRNAs showed that the increase in SOX2 expression 
      and SP cell ratio depends on DNAJB8 and that the increase in DNAJB8 and SOX2 
      depend on HSF1. Furthermore, treatment with a mammalian target of rapamycin 
      (mTOR) inhibitor, temsirolimus, decreased the expression of DNAJB8 and SOX2 and 
      the ratio of SP cells. Taken together, the results indicate that heat shock 
      induces DNAJB8 by activation of HSF1 and induces cancer stem-like cells.
CI  - (c) 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd 
      on behalf of Japanese Cancer Association.
FAU - Kusumoto, Hiroki
AU  - Kusumoto H
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
AD  - Department of Urology, Wakayama Medical University, Wakayama, Japan.
FAU - Hirohashi, Yoshihiko
AU  - Hirohashi Y
AUID- ORCID: 0000-0002-0608-3914
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Nishizawa, Satoshi
AU  - Nishizawa S
AUID- ORCID: 0000-0002-1475-6381
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
AD  - Department of Urology, Wakayama Medical University, Wakayama, Japan.
FAU - Yamashita, Masamichi
AU  - Yamashita M
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Yasuda, Kazuyo
AU  - Yasuda K
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Murai, Aiko
AU  - Murai A
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Takaya, Akari
AU  - Takaya A
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Mori, Takashi
AU  - Mori T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
AD  - Department of Urology, Wakayama Medical University, Wakayama, Japan.
FAU - Kubo, Terufumi
AU  - Kubo T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Nakatsugawa, Munehide
AU  - Nakatsugawa M
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Kanaseki, Takayuki
AU  - Kanaseki T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Tsukahara, Tomohide
AU  - Tsukahara T
AUID- ORCID: 0000-0002-3678-4359
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Kondo, Toru
AU  - Kondo T
AD  - Division of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido 
      University, Sapporo, Japan.
FAU - Sato, Noriyuki
AU  - Sato N
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Hara, Isao
AU  - Hara I
AUID- ORCID: 0000-0003-4542-4045
AD  - Department of Urology, Wakayama Medical University, Wakayama, Japan.
FAU - Torigoe, Toshihiko
AU  - Torigoe T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20180220
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
RN  - 0 (DNAJB8 protein, human)
RN  - 0 (HSF1 protein, human)
RN  - 0 (HSP40 Heat-Shock Proteins)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Neoplastic
MH  - HSP40 Heat-Shock Proteins/genetics/*metabolism
MH  - Heat Shock Transcription Factors/genetics/*metabolism
MH  - Hot Temperature
MH  - Humans
MH  - Kidney Neoplasms/genetics/*metabolism
MH  - Mice
MH  - Molecular Chaperones/genetics/*metabolism
MH  - Neoplasm Transplantation
MH  - Neoplastic Stem Cells/*cytology/metabolism
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Phosphorylation
MH  - SOXB1 Transcription Factors/genetics/*metabolism
MH  - Side-Population Cells/cytology/metabolism
MH  - Stress, Physiological
MH  - Transcriptional Activation
PMC - PMC5834799
OTO - NOTNLM
OT  - DNAJB8
OT  - HSF1
OT  - cancer stem-like cell
OT  - cellular stress
OT  - kidney cancer
EDAT- 2018/01/10 06:00
MHDA- 2018/03/13 06:00
PMCR- 2018/03/01
CRDT- 2018/01/10 06:00
PHST- 2017/09/27 00:00 [received]
PHST- 2017/12/22 00:00 [revised]
PHST- 2017/12/30 00:00 [accepted]
PHST- 2018/01/10 06:00 [pubmed]
PHST- 2018/03/13 06:00 [medline]
PHST- 2018/01/10 06:00 [entrez]
PHST- 2018/03/01 00:00 [pmc-release]
AID - CAS13501 [pii]
AID - 10.1111/cas.13501 [doi]
PST - ppublish
SO  - Cancer Sci. 2018 Mar;109(3):741-750. doi: 10.1111/cas.13501. Epub 2018 Feb 20.