PMID- 29317576
OWN - NLM
STAT- MEDLINE
DCOM- 20181024
LR  - 20181024
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 42
IP  - 12
DP  - 2017 Dec 28
TI  - [Protective effect of taxifolin on H2O2-induced 
H9C2 cell pyroptosis].
PG  - 1367-1374
LID - 10.11817/j.issn.1672-7347.2017.12.003 [doi]
AB  - To explore the effect of taxifolin on H2O2-induced pyroptosis in H9C2 cells and 
      the possible mechanisms.
 Methods: The H9C2 cells was divided into 3 groups: a 
      control group, a hydrogen peroxide (H2O2)group and a taxifolin group. The 
      morphology of H9C2 cells was observed by inverted phase contrast microscope. The 
      mitochondrial membrane potential was measured by JC-1 staining and flow 
      cytometry. The alteration of the level of reactive oxygen species (ROS) was 
      detected by specific mitochondrial probe. The protein levels of cysteinyl 
      aspartate specific proteinase-1 (caspase-1)was determined by Western blot. The 
      mRNA levels of interleukin-18 (IL-18), interleukin-1a (IL-1a), interleukin-1b 
      (IL-1b), absent in melanoma 2 (AIM2), apoptosis-associated apeck-like protein 
      (ASC), nucleotide-binding oligomerization domain-like receptor protein 3 
      (NLRP3)and nucleotide-binding oligomerization domain-like receptor family caspase 
      recruitment domain-containing protein 4 (NLRC4) were determined by reverse 
      transcription-polymerase chain reaction (RT-PCR).
 Results: Compared with the 
      control group, the morphology of H9C2 cells obviously changed in the H2O2-treated 
      group, which was guadually improved in the presence of taxifolin. Compared with 
      the control group, the mitochondrial membrane potential was markedly decreased in 
      the H2O2-treated cells, accompanied by the increase ofROS (both P<0.05). Compared 
      with the H2O2 group, the mitochondrial membrane potential changes in the 
      taxifolin group was increased while the ROS was decreased, with significant 
      difference (both P<0.05). Compared with the control group, the protein level of 
      caspase-1 and the mRNA levels of IL-18, IL-1a, IL-1b, AIM2, ASC, NLRP3 and NLRC4 
      in the H2O2-treated group were significantly increased (all P<0.05), which were 
      attenuated in the presence of taxifolin (all P<0.05).
 Conclusion: Taxifolin can 
      protect H9C2 cells from oxidative injury, and it is able to suppress the 
      H2O2-induced H9C2 cell pyroptosis through inhibition of AIM2, NLRP3 and NLRC4 in 
      flammasome.
FAU - Ye, Yanqiong
AU  - Ye Y
AD  - Graduate School, Guangdong Medical University, Zhanjiang Guangdong 524023; 
      Department of Cardiac Surgery , Guangdong General Hospital, Guangzhou 510080, 
      China.
FAU - Wang, Xiaoli
AU  - Wang X
AD  - Center for Heart Development, School of Life Sciences, Hunan Normal University, 
      Changsha 410006, China.
FAU - Cai, Qian
AU  - Cai Q
AD  - Department of Pediatrics, Third Xiangya Hospital, Central South University, 
      Changsha 410013, China.
FAU - Zhuang, Jian
AU  - Zhuang J
AD  - Department of Cardiac Surgery , Guangdong General Hospital, Guangzhou 510080, 
      China.
FAU - Tan, Xiaohua
AU  - Tan X
AD  - Department of Morphology, School of Basic Medicine, Central South University, 
      Changsha 410013, China.
FAU - He, Wei
AU  - He W
AD  - Department of Pediatrics, Third Xiangya Hospital, Central South University, 
      Changsha 410013, China.
FAU - Zhao, Mingyi
AU  - Zhao M
AD  - Department of Pediatrics, Third Xiangya Hospital, Central South University, 
      Changsha 410013; Department of Morphology, School of Basic Medicine, Central 
      South University, Changsha 410013, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (AIM2 protein, rat)
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (CARD Signaling Adaptor Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukins)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRC4 protein, rat)
RN  - 0 (Nlrp3 protein, rat)
RN  - 0 (Pycard protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Cell Surface)
RN  - 9IKM0I5T1E (Quercetin)
RN  - 9SOB9E3987 (taxifolin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - CARD Signaling Adaptor Proteins/analysis
MH  - Caspase 1/analysis
MH  - Cell Line
MH  - DNA-Binding Proteins/analysis
MH  - *Hydrogen Peroxide/antagonists & inhibitors
MH  - Interleukins/analysis
MH  - Membrane Potential, Mitochondrial/*drug effects
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/analysis
MH  - Pyroptosis/*drug effects
MH  - Quercetin/*analogs & derivatives/pharmacology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Reactive Oxygen Species/analysis
MH  - Receptors, Cell Surface/analysis
EDAT- 2018/01/11 06:00
MHDA- 2018/10/26 06:00
CRDT- 2018/01/11 06:00
PHST- 2018/01/11 06:00 [entrez]
PHST- 2018/01/11 06:00 [pubmed]
PHST- 2018/10/26 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2017.12.003 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2017 Dec 28;42(12):1367-1374. doi: 
      10.11817/j.issn.1672-7347.2017.12.003.