PMID- 29318853
OWN - NLM
STAT- MEDLINE
DCOM- 20190403
LR  - 20190403
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 42
IP  - 6
DP  - 2017 Dec 25
TI  - [Effects of Electroacupuncture on Adaptive Hypertrophy of Gastrocnemius and 
      Proliferation and Differentiation of Muscle Satellite Cells in Rats].
PG  - 489-95
LID - 10.13702/j.1000-0607.2017.06.004 [doi]
AB  - OBJECTIVE: To explore the effects and mechanism of different duration 
      electroacupuncture (EA) on adaptive hypertrophy of gastrocnemius and the 
      expressions of proliferation cell nuclear antigen (PCNA), paired box 
      transcription factor 7 (Pax 7), myogenic differentiation antigen (MyoD 1), 
      myogenin (MyoG), myosin heavy chain-Ⅰ(Myh 7), transforming growth factor-beta 1 
      (TGF-beta 1), Smad 3 in rats. METHODS: Twenty-four SD adult male rats were randomly 
      divided into normal, two-week EA (A 2), four-week EA (A 4) and eight-week EA (A 
      8) groups, 6 rats in each group. Except the normal group, EA was applied at right 
      "Zusanli" (ST 36) and "Huantiao" (GB 30) for 10 min in the other three groups, 
      separately for 2 weeks, 4 weeks and 8 weeks, once a day, 6 times a week. The 
      ratio of gastrocnemius wet weight was calculated after EA. The cross-sectional 
      area and diameter of muscle fiber were measured after HE staining. The expression 
      of PCNA protein was detected by immunofluorescence. The relative expressions of 
      Pax 7, MyoD 1, MyoG, Myh 7, TGF-beta 1 and Smad 3 mRNA were tested with real-time 
      PCR. RESULTS: The expressions of PCNA protein were obviously higher in the A 2 
      and A 4 groups than that in the normal group. The expression of genes in the EA 
      intervention groups showed a trend of increasing first and then decreasing except 
      Smad 3 mRNA. The relative expressions of Pax 7 and TGF-beta 1 mRNAs reached their 
      peaks in the A 2 group compared with those in the normal group (P<0.01). The 
      ratio of gastrocnemius wet weight, cross-sectional area and diameter of muscle 
      fiber, and the relative expressions of MyoD 1, MyoG, Myh 7 mRNAs reached their 
      peaks in the A 4 group (P<0.01), while the relative expression of Smad 3 mRNA 
      showed a trend of decreasing first and then increasing and reached its bottom at 
      the 4(th) week (P<0.01). CONCLUSIONS: EA at "Zusanli" (ST 36) and "Huantiao" (GB 
      30) may induce adaptive hypertrophy of gastrocnemius in rats by up-regulating the 
      expressions of Pax 7, PCNA, TGF-beta 1, MyoD 1, MyoG, Myh 7 and down-regulating the 
      expression of Smad 3, promoting the proliferation of skeletal satellite cells, 
      myogenic differentiation, myotubes terminal differentiation, increasing the wet 
      weight, fiber cross-sectional area and diameter of gastrocnemius.
FAU - Zhao, Dan-Dan
AU  - Zhao DD
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
FAU - Tang, Cheng-Lin
AU  - Tang CL
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
FAU - Huang, Si-Qin
AU  - Huang SQ
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
FAU - Luo, Ao
AU  - Luo A
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
FAU - Zhang, An-Ning
AU  - Zhang AN
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
FAU - Guo, Quan-Hu
AU  - Guo QH
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
FAU - Cao, Jing
AU  - Cao J
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
FAU - Gao, Rui-Qi
AU  - Gao RQ
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China; Chongqing Key Laboratory of Traditional Chinese Medicine 
      for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
SB  - IM
MH  - Acupuncture Points
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - *Electroacupuncture
MH  - Hypertrophy/therapy
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Electroacupuncture
OT  - Hypertrophy of skeletal muscle
OT  - Muscle satellite cells
OT  - Myogenic differentiation antigen
OT  - Paired box transcription factor 7
EDAT- 2018/01/11 06:00
MHDA- 2019/04/04 06:00
CRDT- 2018/01/11 06:00
PHST- 2018/01/11 06:00 [entrez]
PHST- 2018/01/11 06:00 [pubmed]
PHST- 2019/04/04 06:00 [medline]
AID - 10.13702/j.1000-0607.2017.06.004 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2017 Dec 25;42(6):489-95. doi: 10.13702/j.1000-0607.2017.06.004.