PMID- 29322544
OWN - NLM
STAT- MEDLINE
DCOM- 20190612
LR  - 20220321
IS  - 1365-2036 (Electronic)
IS  - 0269-2813 (Linking)
VI  - 47
IP  - 6
DP  - 2018 Mar
TI  - Role of bisphenol A as environmental factor in the promotion of non-alcoholic 
      fatty liver disease: in vitro and clinical study.
PG  - 826-837
LID - 10.1111/apt.14499 [doi]
AB  - BACKGROUND: Bisphenol A is an endocrine disrupting chemical associated with type 
      2 diabetes mellitus (T2DM), cardiovascular disease and liver enzyme 
      abnormalities. AIM: To evaluate bisphenol A plasma and urine levels in 
      non-alcoholic fatty liver disease (NAFLD) patients compared to healthy subjects. 
      Furthermore, we evaluated, in human HepG2 cells, the effects of exposure to 
      different concentrations of bisphenol A on both oxidative stress induction and 
      cell proliferation. METHODS: We enrolled 60 patients with histological diagnosis 
      of NAFLD with or without T2DM and sixty healthy subjects. In vitro, the 
      proliferation of bisphenol A-exposed HepG2 cells at two different concentrations 
      (0.025 and 0.05 muM) was evaluated, both at high (H-HepG2) and at low (L-HepG2) 
      glucose concentrations for 48 h. Lipoperoxidation was assessed by thiobarbituric 
      acid reactive substances (TBARS) assay. RESULTS: Bisphenol A levels were 
      significantly higher in 60 NAFLD subjects, both in urine and in plasma 
      (P < 0.0001) when compared to controls and, in this group, it appeared to be 
      higher in 30 non-alcoholic steatohepatitis patients compared to 30 simple 
      steatosis subjects (P < 0.05), independently from the presence of T2DM. After a 
      bisphenol A-free diet for 1 month, NAFLD patients showed a significant reduction 
      in bisphenol A circulating levels (P < 0.05), without a significant reduction in 
      urine levels. H-HepG2 cells treated with bisphenol A (0.05 muM) increased 
      proliferation compared to controls at 48 h (P < 0.0001). Bisphenol A increased 
      TBARS levels at 48 h versus controls. CONCLUSIONS: Our study reveals a possible 
      role of bisphenol A as an environmental factor involved in the promotion of 
      NAFLD, particularly in T2DM patients.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Dallio, M
AU  - Dallio M
AD  - Department of Clinical and Experimental Medicine, University of Campania "Luigi 
      Vanvitelli", Naples, Italy.
FAU - Masarone, M
AU  - Masarone M
AD  - Department of Medicine and Surgery, University of Salerno, Baronissi, Salerno, 
      Italy.
FAU - Errico, S
AU  - Errico S
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      Naples, Italy.
FAU - Gravina, A G
AU  - Gravina AG
AD  - Department of Clinical and Experimental Medicine, University of Campania "Luigi 
      Vanvitelli", Naples, Italy.
FAU - Nicolucci, C
AU  - Nicolucci C
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      Naples, Italy.
FAU - Di Sarno, R
AU  - Di Sarno R
AD  - Department of Clinical and Experimental Medicine, University of Campania "Luigi 
      Vanvitelli", Naples, Italy.
FAU - Gionti, L
AU  - Gionti L
AD  - Department of Clinical and Experimental Medicine, University of Campania "Luigi 
      Vanvitelli", Naples, Italy.
FAU - Tuccillo, C
AU  - Tuccillo C
AD  - Department of Clinical and Experimental Medicine, University of Campania "Luigi 
      Vanvitelli", Naples, Italy.
FAU - Persico, M
AU  - Persico M
AUID- ORCID: 0000-0002-1399-6498
AD  - Department of Medicine and Surgery, University of Salerno, Baronissi, Salerno, 
      Italy.
FAU - Stiuso, P
AU  - Stiuso P
AD  - Department of Biochemistry, Biophysics and General Pathology, University of 
      Campania "Luigi Vanvitelli", Naples, Italy.
FAU - Diano, N
AU  - Diano N
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      Naples, Italy.
FAU - Loguercio, C
AU  - Loguercio C
AD  - Department of Clinical and Experimental Medicine, University of Campania "Luigi 
      Vanvitelli", Naples, Italy.
FAU - Federico, A
AU  - Federico A
AUID- ORCID: 0000-0002-0885-0793
AD  - Department of Clinical and Experimental Medicine, University of Campania "Luigi 
      Vanvitelli", Naples, Italy.
LA  - eng
PT  - Clinical Study
PT  - Journal Article
DEP - 20180111
PL  - England
TA  - Aliment Pharmacol Ther
JT  - Alimentary pharmacology & therapeutics
JID - 8707234
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fatty Acids)
RN  - 0 (Phenols)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Benzhydryl Compounds/*toxicity
MH  - Case-Control Studies
MH  - Cell Proliferation/drug effects
MH  - Diabetes Mellitus, Type 2/complications/epidemiology
MH  - Environmental Pollutants/toxicity
MH  - Fatty Acids/pharmacology
MH  - Female
MH  - Hep G2 Cells
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Non-alcoholic Fatty Liver Disease/*chemically 
      induced/complications/*epidemiology/pathology
MH  - Phenols/*toxicity
EDAT- 2018/01/13 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/01/12 06:00
PHST- 2017/05/17 00:00 [received]
PHST- 2017/06/24 00:00 [revised]
PHST- 2017/12/10 00:00 [accepted]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/01/12 06:00 [entrez]
AID - 10.1111/apt.14499 [doi]
PST - ppublish
SO  - Aliment Pharmacol Ther. 2018 Mar;47(6):826-837. doi: 10.1111/apt.14499. Epub 2018 
      Jan 11.