PMID- 29323730
OWN - NLM
STAT- MEDLINE
DCOM- 20190417
LR  - 20190417
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 119
IP  - 7
DP  - 2018 Jul
TI  - Biochemical and molecular study on interleukin-1beta gene expression and relation of 
      single nucleotide polymorphism in promoter region with Type 2 diabetes mellitus.
PG  - 5343-5349
LID - 10.1002/jcb.26667 [doi]
AB  - Interleukin-1beta (IL-1beta) assumes a centric role in the regulation of immune and 
      inflammatory responses and thus has been recognized in immune mediated diseases 
      like type 2 diabetes mellitus (T2DM). We aimed to investigate expressed level of 
      IL-1beta and its relation with IL-1beta -511T>C polymorphism in T2DM patients. This 
      study enrolled 80 subjects (50 patients with T2DM and 30 healthy control 
      subjects). Laboratory investigations included fasting (FBG) and 2 h postprandial 
      blood sugar (2 h PBG), HBA1c, lipid profile, and renal function tests. Genotyping 
      of IL-1beta -511T>C (rs16944) SNP assay by real-time PCR and relative quantitation 
      of IL-1beta gene expression transcript by real-time PCR. RESULTS: T2DM patients had 
      significantly higher FBG and 2 h PBG, HBA1c, LDLc, TC, TG, systolic, and 
      diastolic BP while lower HDLc compared with control group. IL 1- beta -511 T>C, CC 
      genotype and C allele were significantly associated with risk of T2DM with odds 
      ratio (OR) 4.73, 95%CI (1.21-18.39) and OR 2.27, 95%CI (1.72-4.40), respectively. 
      Moreover, diabetic patients had significantly higher IL 1- beta gene transcript 
      compared with control group (P < 0.001). CC genotype of IL 1- beta -511 T > C had 
      the highest significant level of IL 1- beta gene transcript demonstrated compared 
      with C/T and T/T genotypes (P < 0.001) in patients. CONCLUSION: C allele of IL-1 
      beta -511 T >C could be considered risk factor contributor to T2DM and excess level 
      of IL-1 beta transcript may disclose to some degree the inflammatory role of 
      cytokines in T2DM.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Tayel, Safaa I
AU  - Tayel SI
AUID- ORCID: 0000-0002-6528-0256
AD  - Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, 
      Menoufia University, Shebin El-Kom, Egypt.
FAU - Fouda, Eman A M
AU  - Fouda EAM
AD  - Department of Biochemistry, Faculty of Science, Menoufia University, Shebin 
      El-Kom, Egypt.
FAU - Elshayeb, Elsayed I
AU  - Elshayeb EI
AD  - Department of Internal Medicine, Faculty of Medicine, Menoufia University, Shebin 
      El-Kom, Egypt.
FAU - Eldakamawy, Asmaa R A
AU  - Eldakamawy ARA
AD  - Bachelor of Chemistry, Faculty of Science, Menoufia University, Shebin El-Kom, 
      Egypt.
FAU - El-Kousy, Salah M
AU  - El-Kousy SM
AD  - Department of Organic Chemistry, Faculty of Science, Menoufia University, Shebin 
      El-Kom, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20180325
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Interleukin-1beta)
SB  - IM
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Interleukin-1beta/*genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
MH  - Prognosis
MH  - *Promoter Regions, Genetic
MH  - Risk Factors
OTO - NOTNLM
OT  - Type 2 diabetes mellitus
OT  - gene transcript
OT  - interleukin-1beta
OT  - polymorphism
OT  - real time PCR
EDAT- 2018/01/13 06:00
MHDA- 2019/04/18 06:00
CRDT- 2018/01/12 06:00
PHST- 2017/09/25 00:00 [received]
PHST- 2018/01/09 00:00 [accepted]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2019/04/18 06:00 [medline]
PHST- 2018/01/12 06:00 [entrez]
AID - 10.1002/jcb.26667 [doi]
PST - ppublish
SO  - J Cell Biochem. 2018 Jul;119(7):5343-5349. doi: 10.1002/jcb.26667. Epub 2018 Mar 
      25.