PMID- 29324359
OWN - NLM
STAT- MEDLINE
DCOM- 20180601
LR  - 20180601
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 516
DP  - 2018 Mar
TI  - Progress toward an enhanced vaccine: Eight marked attenuated viruses to porcine 
      reproductive and respiratory disease virus.
PG  - 30-37
LID - S0042-6822(17)30441-5 [pii]
LID - 10.1016/j.virol.2017.12.029 [doi]
AB  - Recombinant viruses of strain Ingelvac(R) PRRS porcine reproductive and respiratory 
      syndrome virus (PRRSV) modified live virus vaccine were produced with two 
      individual small in-frame deletions in nonstructural protein 2 (nsp2; Delta23 and 
      Delta87) and also the same deletions supplanted with foreign tags (Delta23-V5, Delta23-FLAG, 
      Delta23-S, Delta87-V5, Delta87-FLAG, Delta87-S). The viruses, but one (Delta87-FLAG), were stable for 
      10 passages and showed minimal effects on in vitro growth. Northern hybridization 
      showed that the Delta23-tagged probe detected intracellular viral genome RNA as well 
      as shorter RNAs that may represent heteroclite species, while the Delta87-tagged 
      probe detected predominantly only genome length RNAs. When the tagged viruses 
      were used to probe nsp2 protein in infected cells, perinuclear localization 
      similar to native nsp2 was seen. Dual infection of Delta23-S and Delta87-S viruses 
      allowed some discrimination of individual tagged nsp2 protein, facilitating 
      future research. The mutants could potentially also be used to differentiate 
      infected from vaccinated animals.
CI  - Published by Elsevier Inc.
FAU - Spear, Allyn
AU  - Spear A
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA. Electronic address: Allyn.Spear@elanco.com.
FAU - Wang, Feng-Xue
AU  - Wang FX
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA. Electronic address: wangfx_vet@163.com.
FAU - Kappes, Matthew A
AU  - Kappes MA
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA. Electronic address: kappes_matthew@bah.com.
FAU - Das, Phani B
AU  - Das PB
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA. Electronic address: phanidas1@yahoo.com.
FAU - Faaberg, Kay S
AU  - Faaberg KS
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA. Electronic address: kay.faaberg@ars.usda.gov.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180108
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*immunology/physiology
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/genetics/*immunology
MH  - Viral Nonstructural Proteins/administration & dosage/genetics/immunology
MH  - Viral Vaccines/administration & dosage/genetics/*immunology
OTO - NOTNLM
OT  - Engineered virus
OT  - FLAG tag
OT  - Modified live vaccine (MLV)
OT  - Nonstructural protein 2 (nsp2)
OT  - Pancreatic ribonuclease A epitope (S-Tag)
OT  - Porcine reproductive and respiratory virus (PRRSV)
OT  - Simian virus 5 epitope (V5)
EDAT- 2018/01/13 06:00
MHDA- 2018/06/02 06:00
CRDT- 2018/01/12 06:00
PHST- 2017/09/29 00:00 [received]
PHST- 2017/12/21 00:00 [revised]
PHST- 2017/12/25 00:00 [accepted]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2018/06/02 06:00 [medline]
PHST- 2018/01/12 06:00 [entrez]
AID - S0042-6822(17)30441-5 [pii]
AID - 10.1016/j.virol.2017.12.029 [doi]
PST - ppublish
SO  - Virology. 2018 Mar;516:30-37. doi: 10.1016/j.virol.2017.12.029. Epub 2018 Jan 8.