PMID- 29325125
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20200220
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Linking)
VI  - 97
IP  - 4
DP  - 2018 Apr 1
TI  - Age, phosphorus, and 25-hydroxycholecalciferol regulate mRNA expression of 
      vitamin D receptor and sodium-phosphate cotransporter in the small intestine of 
      broiler chickens.
PG  - 1199-1208
LID - 10.3382/ps/pex407 [doi]
AB  - Four experiments were conducted in this study. Experiment 1 was carried out to 
      examine mRNA expressions of nuclear vitamin D receptor (nVDR), membrane vitamin D 
      receptor (mVDR), and type IIb sodium-phosphate cotransporter (NaPi-IIb) in the 
      small intestine of broiler chickens. Experiments 2, 3, and 4 were implemented to 
      evaluate effects of age, non-phytate phosphorus (NPP), and 
      25-hydroxycholecalciferol (25-OH-D3) on mRNA expressions of nVDR, mVDR, and 
      NaPi-IIb in the duodenum of chickens. Results showed that mRNA expression levels 
      of nVDR and NaPi-IIb were highest in the duodenum of 21-day-old broilers, lower 
      in the jejunum, and lowest in the ileum. By contrast, no differences in mRNA 
      expression levels of mVDR were detected among the duodenum, jejunum, and ileum. 
      Age quadratically affected mRNA expressions of nVDR, mVDR, and NaPi-IIb in the 
      duodenum and 25-hydroxylase in the liver of 7- to 42-day-old broilers, with the 
      highest levels observed at 21 d of age. By contrast, age linearly decreased mRNA 
      expression level of 1alpha-hydroxylase in kidneys. Dietary NPP levels quadratically 
      affected mRNA expression levels of nVDR and mVDR in the duodenum and 
      25-hydroxylase in the liver of 21-day-old broilers. The highest mRNA expression 
      levels of nVDR and mVDR and lowest mRNA level of 25-hydroxylase were observed at 
      0.55% NPP. mRNA expression level of NaPi-IIb linearly declined when dietary NPP 
      levels increased from 0.25 to 0.65%. Addition of 12.5 mug/kg of 25-OH-D3 increased 
      mRNA expression level of 1alpha-hydroxylase in kidneys and those of nVDR, mVDR, and 
      NaPi-IIb in the duodenum of broilers compared with birds fed the diet without 
      25-OH-D3. These data indicate that mRNA expressions of nVDR and NaPi-IIb are 
      highest in the duodenum, and the greatest mRNA levels of nVDR, mVDR, and NaPi-IIb 
      are observed at 21 d of age. Dietary NPP levels quadratically increase mRNA 
      expressions of nVDR and mVDR but linearly decrease NaPi-IIb mRNA level. 25-OH-D3 
      up-regulates the above gene transcription.
FAU - Han, J C
AU  - Han JC
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
FAU - Zhang, J L
AU  - Zhang JL
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
AD  - College of Animal Husbandry and Veterinary Science, Henan Agricultural 
      University, Zhengzhou 450002, Henan, China.
FAU - Zhang, N
AU  - Zhang N
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
AD  - College of Animal Husbandry and Veterinary Science, Henan Agricultural 
      University, Zhengzhou 450002, Henan, China.
FAU - Yang, X
AU  - Yang X
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
AD  - College of Animal Husbandry and Veterinary Science, Henan Agricultural 
      University, Zhengzhou 450002, Henan, China.
FAU - Qu, H X
AU  - Qu HX
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
FAU - Guo, Y
AU  - Guo Y
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
FAU - Shi, C X
AU  - Shi CX
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
FAU - Yan, Y F
AU  - Yan YF
AD  - Department of Animal Science, College of Life Science, Shangqiu Normal 
      University, Shangqiu 476000, Henan, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Avian Proteins)
RN  - 0 (Phosphorus, Dietary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Sodium-Phosphate Cotransporter Proteins, Type IIb)
RN  - P6YZ13C99Q (Calcifediol)
SB  - IM
MH  - Age Factors
MH  - Animal Feed/analysis
MH  - Animals
MH  - Avian Proteins/*genetics/metabolism
MH  - Calcifediol/*metabolism
MH  - Chickens/genetics/*physiology
MH  - Diet/veterinary
MH  - Dietary Supplements/analysis
MH  - *Gene Expression Regulation
MH  - Intestine, Small/metabolism
MH  - Male
MH  - Phosphorus, Dietary/administration & dosage/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Random Allocation
MH  - Receptors, Calcitriol/*genetics/metabolism
MH  - Sodium-Phosphate Cotransporter Proteins, Type IIb/*genetics/metabolism
EDAT- 2018/01/13 06:00
MHDA- 2018/08/21 06:00
CRDT- 2018/01/12 06:00
PHST- 2017/08/24 00:00 [received]
PHST- 2017/11/22 00:00 [accepted]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PHST- 2018/01/12 06:00 [entrez]
AID - S0032-5791(19)30985-X [pii]
AID - 10.3382/ps/pex407 [doi]
PST - ppublish
SO  - Poult Sci. 2018 Apr 1;97(4):1199-1208. doi: 10.3382/ps/pex407.