PMID- 29326040
OWN - NLM
STAT- MEDLINE
DCOM- 20180214
LR  - 20180214
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 496
IP  - 2
DP  - 2018 Feb 5
TI  - Downregulation of exocyst Sec10 accelerates kidney tubule cell recovery through 
      enhanced cell migration.
PG  - 309-315
LID - S0006-291X(18)30013-5 [pii]
LID - 10.1016/j.bbrc.2018.01.013 [doi]
AB  - Migration of surviving kidney tubule cells after sub-lethal injury, for example 
      ischemia/reperfusion (I/R), plays a critical role in recovery. Exocytosis is 
      known to be involved in cell migration, and a key component in exocytosis is the 
      highly-conserved eight-protein exocyst complex. We investigated the expression of 
      a central exocyst complex member, Sec10, in kidneys following I/R injury, as well 
      as the role of Sec10 in wound healing following scratch injury of cultured 
      Madin-Darby canine kidney (MDCK) cells. Sec10 overexpression and knockdown (KD) 
      in MDCK cells were used to investigate the speed of wound healing and the 
      mechanisms underlying recovery. In mice, Sec10 decreased after I/R injury, and 
      increased during the recovery period. In cell culture, Sec10 OE inhibited ruffle 
      formation and wound healing, while Sec10 KD accelerated it. Sec10 OE cells had 
      higher amounts of diacylglycerol kinase (DGK) gamma at the leading edge than did 
      control cells. A DGK inhibitor reversed the inhibition of wound healing and 
      ruffle formation in Sec10 OE cells. Conclusively, downregulation of Sec10 
      following I/R injury appears to accelerate recovery of kidney tubule cells 
      through activated ruffle formation and enhanced cell migration.
CI  - Copyright (c) 2018 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Noh, Mi Ra
AU  - Noh MR
AD  - Department of Anatomy and BK21 Plus, Kyungpook National University School of 
      Medicine, Daegu, Republic of Korea.
FAU - Jang, Hee-Seong
AU  - Jang HS
AD  - Department of Anatomy and BK21 Plus, Kyungpook National University School of 
      Medicine, Daegu, Republic of Korea.
FAU - Song, Dae-Kyu
AU  - Song DK
AD  - Department of Physiology, Keimyung University School of Medicine, Daegu, Republic 
      of Korea; Medical Research Center, Keimyung University School of Medicine, Daegu, 
      Republic of Korea.
FAU - Lee, Seong-Ryong
AU  - Lee SR
AD  - Medical Research Center, Keimyung University School of Medicine, Daegu, Republic 
      of Korea; Department of Pharmacology, Keimyung University School of Medicine, 
      Daegu, Republic of Korea.
FAU - Lipschutz, Joshua H
AU  - Lipschutz JH
AD  - Department of Medicine, Medical University of South Carolina and Ralph H. Johnson 
      Veterans Affairs Medical Center, Charleston, SC, USA.
FAU - Park, Kwon Moo
AU  - Park KM
AD  - Department of Anatomy and BK21 Plus, Kyungpook National University School of 
      Medicine, Daegu, Republic of Korea. Electronic address: kmpark@knu.ac.kr.
FAU - Kim, Jee In
AU  - Kim JI
AD  - Medical Research Center, Keimyung University School of Medicine, Daegu, Republic 
      of Korea; Department of Molecular Medicine Keimyung University School of 
      Medicine, Daegu, Republic of Korea. Electronic address: jeein.kim@kmu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180108
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (EXOC5 protein, mouse)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Piperidines)
RN  - 0 (Quinazolinones)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Vesicular Transport Proteins)
RN  - 120166-69-0 (R 59949)
RN  - EC 2.7.1.107 (Diacylglycerol Kinase)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Diacylglycerol Kinase/*antagonists & inhibitors/genetics/metabolism
MH  - Dogs
MH  - Enzyme Inhibitors/pharmacology
MH  - Exocytosis
MH  - Gene Expression Regulation
MH  - Kidney Tubules/*metabolism/pathology
MH  - Madin Darby Canine Kidney Cells
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Piperidines/pharmacology
MH  - Quinazolinones/pharmacology
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Reperfusion Injury/genetics/metabolism/pathology/*prevention & control
MH  - Vesicular Transport Proteins/agonists/antagonists & 
      inhibitors/*genetics/metabolism
MH  - Wound Healing/physiology
OTO - NOTNLM
OT  - DGK gamma
OT  - Exocyst Sec10
OT  - Kidney tubule cell
OT  - Migration
OT  - Ruffle formation
OT  - Wound healing
EDAT- 2018/01/13 06:00
MHDA- 2018/02/15 06:00
CRDT- 2018/01/13 06:00
PHST- 2017/12/22 00:00 [received]
PHST- 2018/01/03 00:00 [accepted]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2018/02/15 06:00 [medline]
PHST- 2018/01/13 06:00 [entrez]
AID - S0006-291X(18)30013-5 [pii]
AID - 10.1016/j.bbrc.2018.01.013 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Feb 5;496(2):309-315. doi: 
      10.1016/j.bbrc.2018.01.013. Epub 2018 Jan 8.