PMID- 29326671
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Specificity Characterization of SLA Class I Molecules Binding to Swine-Origin 
      Viral Cytotoxic T Lymphocyte Epitope Peptides in Vitro.
PG  - 2524
LID - 10.3389/fmicb.2017.02524 [doi]
LID - 2524
AB  - Swine leukocyte antigen (SLA) class I molecules play a crucial role in generating 
      specific cellular immune responses against viruses and other intracellular 
      pathogens. They mainly bind and present antigens of intracellular origin to 
      circulating MHC I-restricted cytotoxic T lymphocytes (CTLs). Binding of an 
      appropriate epitope to an SLA class I molecule is the single most selective event 
      in antigen presentation and the first step in the killing of infected cells by 
      CD8+ CTLs. Moreover, the antigen epitopes are strictly restricted to specific SLA 
      molecules. In this study, we constructed SLA class I complexes in vitro 
      comprising viral epitope peptides, the extracellular region of the SLA-1 
      molecules, and beta2-microglobulin (beta2m) using splicing overlap extension polymerase 
      chain reaction (SOE-PCR). The protein complexes were induced and expressed in an 
      Escherichia coli prokaryotic expression system and subsequently purified and 
      refolded. Specific binding of seven SLA-1 proteins to one classical swine fever 
      virus (CSFV) and four porcine reproductive and respiratory syndrome virus (PRRSV) 
      epitope peptides was detected by enzyme-linked immunosorbent assay (ELISA)-based 
      method. The SLA-1( *)13:01, SLA-1( *)11:10, and SLA-1( *)11:01:02 proteins were able 
      to bind specifically to different CTL epitopes of CSFV and PRRSV and the MHC 
      restrictions of the five epitopes were identified. The fixed combination of 
      Asn(151)Val(152) residues was identified as the potentially key amino acid 
      residues influencing the binding of viral several CTL epitope peptides to 
      SLA-1( *)13:01 and SLA-1( *)04:01:01 proteins. The more flexible pocket E in the 
      SLA-1( *)13:01 protein might have fewer steric limitations and therefore be able 
      to accommodate more residues of viral CTL epitope peptides, and may thus play a 
      critical biochemical role in determining the peptide-binding motif of 
      SLA-1( *)13:01. Characterization of the binding specificity of peptides to SLA 
      class I molecules provides an important basis for epitope studies of infectious 
      diseases in swine, and for the rational development of novel porcine vaccines, as 
      well as for detailed studies of CTL responses in pigs used as animal models.
FAU - Gao, Caixia
AU  - Gao C
AD  - Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative 
      Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - He, Xiwen
AU  - He X
AD  - Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative 
      Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Quan, Jinqiang
AU  - Quan J
AD  - Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative 
      Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Jiang, Qian
AU  - Jiang Q
AD  - Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative 
      Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Lin, Huan
AU  - Lin H
AD  - Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative 
      Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Chen, Hongyan
AU  - Chen H
AD  - Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative 
      Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Qu, Liandong
AU  - Qu L
AD  - Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative 
      Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20171218
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5741678
OTO - NOTNLM
OT  - CTL epitope peptide
OT  - MHC restriction
OT  - major histocompatibility complex
OT  - specific binding
OT  - swine leukocyte antigen
EDAT- 2018/01/13 06:00
MHDA- 2018/01/13 06:01
PMCR- 2017/12/18
CRDT- 2018/01/13 06:00
PHST- 2017/08/24 00:00 [received]
PHST- 2017/12/05 00:00 [accepted]
PHST- 2018/01/13 06:00 [entrez]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2018/01/13 06:01 [medline]
PHST- 2017/12/18 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.02524 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Dec 18;8:2524. doi: 10.3389/fmicb.2017.02524. eCollection 
      2017.