PMID- 29326720
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 8
DP  - 2017
TI  - Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine 
      Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs.
PG  - 1846
LID - 10.3389/fimmu.2017.01846 [doi]
LID - 1846
AB  - Vaccination is the most reliable measure to prevent infectious diseases in 
      domestic animals. Development of novel vaccines demands extensive studies with 
      new technologies, such as using novel adjuvants and immunomodulatory molecules. 
      The co-stimulatory molecule 4-1BB provides a key signal that directs the fate of 
      T cells during activation, and thus is important to their function in immune 
      protection. To determine whether host immune responses to viral infection could 
      be promoted by enhancing 4-1BB co-stimulation, in this study, we produced 
      transgenic pig clones expressing an extra copy of the 4-1BB gene by clustered 
      regularly interspaced short palindromic repeats/CRISPR-associated gene 9-mediated 
      homologous recombination at the Rosa26 locus. The immune responses of transgenic 
      pigs to porcine reproductive and respiratory syndrome virus (PRRSV) vaccine were 
      determined on day 14. We show that peripheral blood lymphocytes of transgenic 
      pigs expressed around twice the level of 4-1BB mRNA than those of control pigs. 
      We also found IL-2, TNF-alpha, and granzyme B mRNA levels as well as PRRSV-specific 
      IFN-gamma response were significantly upregulated in 4-1BB transgenic pigs, leading 
      to more efficient cytotoxic T lymphocyte (CTL) killing, whereas the expressions 
      of IL-4, IL-17, and Foxp3 were not affected. These results indicate that higher 
      levels of 4-1BB expression involve in promoting Th1 differentiation and enhancing 
      specific CTL responses to PRRSV, and provide a novel approach to increase the 
      efficacy of current vaccines to control the infectious diseases.
FAU - Huang, Guangping
AU  - Huang G
AD  - State Key Laboratory for Agrobiotechnology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Liu, Xianyong
AU  - Liu X
AD  - State Key Laboratory for Agrobiotechnology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Duszynski, Donal W
AU  - Duszynski DW
AD  - Department of Biology, University of New Mexico, Albuquerque, NM, United States.
FAU - Tang, Xiaoli
AU  - Tang X
AD  - State Key Laboratory for Agrobiotechnology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - El-Ashram, Saeed
AU  - El-Ashram S
AD  - State Key Laboratory for Agrobiotechnology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Liu, Zhengzhu
AU  - Liu Z
AD  - Department of Animal Science and Veterinary Medicine, Hebei Normal University of 
      Science and Technology, Qinhuangdao, Hebei, China.
FAU - Suo, Xun
AU  - Suo X
AD  - State Key Laboratory for Agrobiotechnology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, China.
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Li, Qiuyan
AU  - Li Q
AD  - State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20171218
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC5741594
OTO - NOTNLM
OT  - 4-1BB
OT  - co-stimulation
OT  - immune response
OT  - transgenic pig
OT  - viral infection
EDAT- 2018/01/13 06:00
MHDA- 2018/01/13 06:01
PMCR- 2017/01/01
CRDT- 2018/01/13 06:00
PHST- 2017/06/09 00:00 [received]
PHST- 2017/12/06 00:00 [accepted]
PHST- 2018/01/13 06:00 [entrez]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2018/01/13 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2017.01846 [doi]
PST - epublish
SO  - Front Immunol. 2017 Dec 18;8:1846. doi: 10.3389/fimmu.2017.01846. eCollection 
      2017.