PMID- 29329680
OWN - NLM
STAT- MEDLINE
DCOM- 20190325
LR  - 20190325
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 196
DP  - 2018 Apr
TI  - Correlation of severity of chronic obstructive pulmonary disease with potential 
      biomarkers.
PG  - 1-10
LID - S0165-2478(17)30316-4 [pii]
LID - 10.1016/j.imlet.2018.01.004 [doi]
AB  - BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a non-specific 
      inflammation, which involves the airways, lung parenchyma and pulmonary vessels. 
      The inflammation causes the activation of inflammatory cells and the release of 
      various inflammatory mediators such as interleukin-1 beta, interleukin-6 and 
      tumor necrosis factor alpha (TNF-a). The present study was designed to assess the 
      serum cytokines [Interleukin-1beta (IL-1beta), Interleukin-6 (IL-6), Tumor necrosis 
      factor-alpha (TNF-alpha)] levels in chronic obstructive pulmonary disease (COPD) patients 
      and they were correlated with severity of disease by spirometric measurements. 
      MATERIALS AND METHODS: A total of 384 COPD patients and 50 healthy controls were 
      enrolled in this study. The COPD patients were divided according to gold stages 
      ie: mild, moderate, severe and very severe. 5 ml of venous blood samples were 
      taken from all participants and it was collected in a test tube containing 
      anticoagulant and then centrifuged at 3000 rpm for 10 min. Serum was separated 
      and used to measure the amount of TNF-alpha, il-1beta, and IL-6. Spirometry was 
      performed according to the criteria set by the Gold 2012 RESULTS: Tnf-alpha (pg/ml), 
      IL-6 (pg/ml), IL-1beta (pg/ml) serum levels in COPD patients and healthy controls 
      subjects were measured. Tnf-alpha and IL-6 serum levels were significantly (<0.001) 
      higher in COPD patients compared to healthy control subjects. Likewise, IL-1 beta 
      levels were also significantly (p-value = 0.022) higher in COPD patients compared 
      to healthy control subjects. The distribution of Tnf-alpha, IL-6, IL-1beta (pg/ml) serum 
      levels in COPD patients in relation to GOLD grading. There was a significant 
      (p < 0.001) difference in the level of TNF-alpha, IL-6 and IL-1beta (pg/ml) among the 
      severity of COPD. The posthoc analysis revealed that the TNF-alpha was significantly 
      (p < 0.05) higher among the than mild, moderate, severe and very severe COPD 
      patients. A similar observation was also found for IL-6. However, IL-6 was 
      significantly (p < 0.05) higher among mild, moderate, severe and very severe COPD 
      patients. There was significant (p = < 0.0001) difference in the level of IL-1beta 
      in the different severity of COPD. The posthoc comparison test showed that IL-1beta 
      levels were significantly (p < 0.05) higher among the mild, moderate, severe and 
      very severe COPD patients. CONCLUSION: The present study signifies that the 
      levels of TNF-alpha, IL-1beta, and IL-6 are directly proportional to the 
      post-bronchodilator FEV1 percentage. Results provide population-based evidence 
      that COPD is independently associated with low-grade systemic inflammation, with 
      a different inflammatory pattern than that observed in healthy subjects. Overall, 
      these results identify a novel systemic inflammatory COPD phenotype that may be 
      the target of specific research and treatment.
CI  - Copyright (c) 2018 European Federation of Immunological Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Singh, Seema
AU  - Singh S
AD  - Department of Respiratory Medicine, King George Medical University, Lucknow, 
      Uttar Pradesh, 226003, India.
FAU - Verma, S K
AU  - Verma SK
AD  - Department of Respiratory Medicine, King George Medical University, Lucknow, 
      Uttar Pradesh, 226003, India. Electronic address: drskverma@rediffmail.com.
FAU - Kumar, Santosh
AU  - Kumar S
AD  - Department of Respiratory Medicine, King George Medical University, Lucknow, 
      Uttar Pradesh, 226003, India.
FAU - Ahmad, M K
AU  - Ahmad MK
AD  - Department of Biochemistry, King George Medical University, Lucknow, Uttar 
      Pradesh, 226003, India.
FAU - Nischal, Anuradha
AU  - Nischal A
AD  - Department of Pharmacology & Therapeutics, King George Medical University, 
      Lucknow, Uttar Pradesh, 226003, India.
FAU - Singh, S K
AU  - Singh SK
AD  - Department of Community Medicine and Public Health, King George Medical 
      University, Lucknow, Uttar Pradesh, 226003, India.
FAU - Dixit, R K
AU  - Dixit RK
AD  - Department of Pharmacology & Therapeutics, King George Medical University, 
      Lucknow, Uttar Pradesh, 226003, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180109
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/*blood
MH  - Cross-Sectional Studies
MH  - Cytokines/*blood
MH  - Female
MH  - Hospitals, Teaching
MH  - Humans
MH  - Interleukin-1beta/blood
MH  - Interleukin-6/blood
MH  - Male
MH  - Middle Aged
MH  - Pulmonary Disease, Chronic Obstructive/*blood/*pathology
MH  - *Severity of Illness Index
MH  - Tertiary Care Centers
MH  - Tumor Necrosis Factor-alpha/blood
OTO - NOTNLM
OT  - Chronic obstructive pulmonary disease
OT  - Correlation
OT  - Proinflammatory markers
OT  - Spirometric analysis
EDAT- 2018/01/14 06:00
MHDA- 2019/03/26 06:00
CRDT- 2018/01/14 06:00
PHST- 2017/07/07 00:00 [received]
PHST- 2017/11/16 00:00 [revised]
PHST- 2018/01/08 00:00 [accepted]
PHST- 2018/01/14 06:00 [pubmed]
PHST- 2019/03/26 06:00 [medline]
PHST- 2018/01/14 06:00 [entrez]
AID - S0165-2478(17)30316-4 [pii]
AID - 10.1016/j.imlet.2018.01.004 [doi]
PST - ppublish
SO  - Immunol Lett. 2018 Apr;196:1-10. doi: 10.1016/j.imlet.2018.01.004. Epub 2018 Jan 
      9.