PMID- 29330789
OWN - NLM
STAT- MEDLINE
DCOM- 20190111
LR  - 20190111
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1742
DP  - 2018
TI  - Fluorescence Lifetime Imaging Microscopy (FLIM) as a Tool to Investigate 
      Hypoxia-Induced Protein-Protein Interaction in Living Cells.
PG  - 45-53
LID - 10.1007/978-1-4939-7665-2_5 [doi]
AB  - Fluorescence resonance energy transfer (FRET) is widely used as a method to 
      investigate protein-protein interactions in living cells. A FRET pair donor 
      fluorophore in close proximity to an appropriate acceptor fluorophore transfers 
      emission energy to the acceptor, resulting in a shorter lifetime of the donor 
      fluorescence. When the respective FRET donor and acceptor are fused with two 
      proteins of interest, a reduction in donor lifetime, as detected by fluorescence 
      lifetime imaging microscopy (FLIM), can be taken as proof of close proximity 
      between the fluorophores and therefore interaction between the proteins of 
      interest. Here, we describe the usage of time-domain FLIM-FRET in hypoxia-related 
      research when we record the interaction of the hypoxia-inducible factor-1 (HIF-1) 
      subunits HIF-1alpha and HIF-1beta in living cells in a temperature- and CO(2)-controlled 
      environment under the microscope.
FAU - Schutzhold, Vera
AU  - Schutzhold V
AD  - Institute of Physiology, University of Duisburg-Essen, Essen, Germany.
FAU - Fandrey, Joachim
AU  - Fandrey J
AD  - Institute of Physiology, University of Duisburg-Essen, Essen, Germany. 
      joachim.fandrey@uni-due.de.
FAU - Prost-Fingerle, Katrin
AU  - Prost-Fingerle K
AD  - Institute of Physiology, University of Duisburg-Essen, Essen, Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (ARNT protein, human)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
RN  - 142M471B3J (Carbon Dioxide)
SB  - IM
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator/*metabolism
MH  - Carbon Dioxide/metabolism
MH  - Cell Hypoxia
MH  - Fluorescence Resonance Energy Transfer/*methods
MH  - HEK293 Cells
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Microscopy, Fluorescence
MH  - Optical Imaging
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - Temperature
OTO - NOTNLM
OT  - FLIM
OT  - FRET
OT  - Fluorescence lifetime imaging microscopy
OT  - Fluorescence resonance energy transfer
OT  - HIF-1
OT  - Hypoxia-inducible factor-1
OT  - Live cell imaging
OT  - Protein-protein interaction
EDAT- 2018/01/14 06:00
MHDA- 2019/01/12 06:00
CRDT- 2018/01/14 06:00
PHST- 2018/01/14 06:00 [entrez]
PHST- 2018/01/14 06:00 [pubmed]
PHST- 2019/01/12 06:00 [medline]
AID - 10.1007/978-1-4939-7665-2_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1742:45-53. doi: 10.1007/978-1-4939-7665-2_5.