PMID- 29330937
OWN - NLM
STAT- MEDLINE
DCOM- 20180720
LR  - 20181202
IS  - 1865-1682 (Electronic)
IS  - 1865-1674 (Linking)
VI  - 65
IP  - 2
DP  - 2018 Apr
TI  - First-time detection of porcine reproductive and respiratory syndrome virus 
      (PRRSV) infection in Uruguay.
PG  - 352-356
LID - 10.1111/tbed.12813 [doi]
AB  - Within the last two decades, several high-impact viruses have emerged in the 
      global swine population, including porcine reproductive and respiratory syndrome 
      virus (PRRSV). In Uruguay, the more recent serological survey for PRRSV and other 
      notifiable diseases such as Aujeszky's disease virus (ADV) and classical swine 
      fever virus (CSFV) dated from year 2000. The main purpose of this study was to 
      update our information on the infection status of PRRSV, ADV and CSFV in 
      Uruguayan pig herds, in order to keep informed about the epidemiological 
      situation of these notifiable infections in the country. For serological testing, 
      a total of 524 swine serum samples collected during the period 2014-2016 were 
      assayed by commercial ELISAs. Our results revealed the (unexpected) presence of 
      PRRSV antibodies in Uruguayan domestic swine herds and confirmed the absence of 
      ADV and CSFV antibodies in all of the assessed samples. Following such initial 
      finding, PRRSV antibodies were further investigated in 23 retrospective samples 
      collected during 2010-2014. Thirteen of these 23 samples resulted seropositive. 
      Subsequently, a molecular detection approach in frozen serum samples was 
      implemented to confirm PRRSV infection, and viral RNA was identified by reverse 
      transcription-nested polymerase chain reaction (RT-nPCR). Fourteen of 86 
      evaluated 2014-2016 samples resulted positive for viral RNA, while molecular 
      analysis of four retrospective samples also revealed the presence of PRRSV type 
      2. Viral isolation of selected samples was carried out in porcine alveolar 
      macrophages (PAM) and MARC 145 simian kidney cells, and the virus identity was 
      confirmed by cytopathic effect (CPE) and immunofluorescence assay (IFA) using 
      specific monoclonal antibodies for PRRSV nucleocapsid. Data reported here 
      evidence for the first time the circulation of PRRSV type 2 in Uruguay, and 
      retrospective serology results suggest that the virus has been infecting pigs in 
      this country at least since 2011.
CI  - (c) 2018 Blackwell Verlag GmbH.
FAU - Ramos, N
AU  - Ramos N
AUID- ORCID: 0000-0003-1355-9463
AD  - Seccion Virologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, 
      Uruguay.
FAU - Mirazo, S
AU  - Mirazo S
AD  - Seccion Virologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, 
      Uruguay.
FAU - Castro, G
AU  - Castro G
AD  - Area Suinos, Facultad de Veterinaria, Universidad de la Republica, Montevideo, 
      Uruguay.
FAU - Cabrera, K
AU  - Cabrera K
AD  - Asociacion Uruguaya de productores de cerdos, Montevideo, Uruguay.
FAU - Osorio, F
AU  - Osorio F
AD  - Nebraska Center for Virology & School of Veterinary & Biomedical Sciences, 
      University of Nebraska-Lincoln 140 MOLR, Lincoln, NE, USA.
FAU - Arbiza, J
AU  - Arbiza J
AD  - Seccion Virologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, 
      Uruguay.
LA  - eng
PT  - Journal Article
DEP - 20180112
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antibodies, Viral/blood
MH  - Classical Swine Fever Virus/immunology/isolation & purification
MH  - Communicable Diseases, Emerging/diagnosis/*veterinary/virology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Fluorescent Antibody Technique
MH  - Herpesvirus 1, Suid/immunology/isolation & purification
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/epidemiology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - RNA, Viral/genetics
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Retrospective Studies
MH  - Swine
MH  - Uruguay/epidemiology
OTO - NOTNLM
OT  - molecular detection
OT  - porcine reproductive and respiratory syndrome virus
OT  - serology
OT  - uruguay
OT  - virus isolation
EDAT- 2018/01/14 06:00
MHDA- 2018/07/22 06:00
CRDT- 2018/01/14 06:00
PHST- 2017/10/18 00:00 [received]
PHST- 2018/01/14 06:00 [pubmed]
PHST- 2018/07/22 06:00 [medline]
PHST- 2018/01/14 06:00 [entrez]
AID - 10.1111/tbed.12813 [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2018 Apr;65(2):352-356. doi: 10.1111/tbed.12813. Epub 2018 
      Jan 12.