PMID- 29332916
OWN - NLM
STAT- MEDLINE
DCOM- 20180206
LR  - 20180206
IS  - 1349-3299 (Electronic)
IS  - 1349-2365 (Linking)
VI  - 59
IP  - 1
DP  - 2018 Jan 27
TI  - MiR-135a Promotes Inflammatory Responses of Vascular Smooth Muscle Cells From 
      db/db Mice via Downregulation of FOXO1.
PG  - 170-179
LID - 10.1536/ihj.17-040 [doi]
AB  - It has been shown that microRNAs (miRNAs) greatly affect the functions of 
      vascular smooth muscle cells (VSMC), but the effects of mRNAs under diabetic 
      conditions remain unclear.Using a model of diabetic db/db mice, we studied the 
      functions of microRNA-135a (miR-135a) during VSMC dysfunction.Compared to control 
      WT mice, miR-135a expression in VSMC was significantly increased while the level 
      of forkhead box O1 (FOXO1) protein decreased significantly. After transfecting 
      miR-135a mimics into VSMC, the expression of FOXO1 was decreased, while 
      cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) 
      expression levels were increased, thus promoting the interaction between 
      monocytes and WT VSMC. On the other hand, transfection of an miR-135a inhibitor 
      reversed the activated interaction between monocytes and db/db VSMC. The 
      pro-inflammatory responses could also be enhanced by using siRNAs to silence the 
      FOXO1 gene in WT VSMC, suggesting a negative regulatory role of FOXO1. FOXO1 
      siRNAs and miR-135a mimics could both enhance the transcriptional activity of 
      COX-2 promoter. Using chromatin immunoprecipitation, we found that in db/db VSMC, 
      the occupancy in promoter regions of inflammatory genes by FOXO1 was 
      reduced.miR-135a increased the inflammatory responses of VSMC involved in 
      complications of vascular diseases by downregulating the expression of FOXO1.
FAU - Lu, Xiaochun
AU  - Lu X
AD  - Department of Geriatric Cardiology, Chinese People's Liberation Army General 
      Hospital.
FAU - Yin, Dawei
AU  - Yin D
AD  - Department of Geriatric Cardiology, Chinese People's Liberation Army General 
      Hospital.
FAU - Zhou, Bo
AU  - Zhou B
AD  - Department of Geriatrics, the Affiliated Zhongda Hospital of Southeast 
      University.
FAU - Li, Tieling
AU  - Li T
AD  - Department of Cadre Clinic, Chinese People's Liberation Army General Hospital.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20180115
PL  - Japan
TA  - Int Heart J
JT  - International heart journal
JID - 101244240
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Foxo1 protein, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn135 microRNA, mouse)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - DNA/genetics
MH  - Disease Models, Animal
MH  - *Down-Regulation
MH  - Forkhead Box Protein O1/biosynthesis/*genetics
MH  - *Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Inflammation/*genetics/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - MicroRNAs/biosynthesis/*genetics
MH  - Myocytes, Smooth Muscle/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vascular Diseases/*genetics/metabolism/pathology
OTO - NOTNLM
OT  - COX-2
OT  - MCP-1
OT  - Pro-inflammatory
OT  - Vascular disease
EDAT- 2018/01/16 06:00
MHDA- 2018/02/07 06:00
CRDT- 2018/01/16 06:00
PHST- 2018/01/16 06:00 [pubmed]
PHST- 2018/02/07 06:00 [medline]
PHST- 2018/01/16 06:00 [entrez]
AID - 10.1536/ihj.17-040 [doi]
PST - ppublish
SO  - Int Heart J. 2018 Jan 27;59(1):170-179. doi: 10.1536/ihj.17-040. Epub 2018 Jan 
      15.