PMID- 29333385
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2287-1098 (Print)
IS  - 2287-8602 (Electronic)
IS  - 2287-1098 (Linking)
VI  - 22
IP  - 4
DP  - 2017 Dec
TI  - Kaempferol Inhibits Angiogenesis by Suppressing HIF-1alpha and VEGFR2 Activation via 
      ERK/p38 MAPK and PI3K/Akt/mTOR Signaling Pathways in Endothelial Cells.
PG  - 320-326
LID - 10.3746/pnf.2017.22.4.320 [doi]
AB  - Kaempferol has been shown to inhibit vascular formation in endothelial cells. 
      However, the underlying mechanisms are not fully understood. In the present 
      study, we evaluated whether kaempferol exerts antiangiogenic effects by targeting 
      extracellular signal-regulated kinase (ERK)/p38 mitogen-activated protein kinase 
      (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt/mechanistic target of rapamycin 
      (mTOR) signaling pathways in endothelial cells. Endothelial cells were treated 
      with various concentrations of kaempferol for 24 h. Cell viability was determined 
      by the 3- (4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay; 
      vascular formation was analyzed by tube formation, wound healing, and mouse 
      aortic ring assays. Activation of hypoxia-inducible factor-1alpha (HIF-1alpha), vascular 
      endothelial growth factor receptor 2 (VEGFR2), ERK/p38 MAPK, and PI3K/Akt/mTOR 
      was analyzed by Western blotting. Kaempferol significantly inhibited cell 
      migration and tube formation in endothelial cells, and suppressed microvessel 
      sprouting in the mouse aortic ring assay. Moreover, kaempferol suppressed the 
      activation of HIF-1alpha, VEGFR2, and other markers of ERK/p38 MAPK and PI3K/Akt/mTOR 
      signaling pathways in endothelial cells. These results suggest that kaempferol 
      inhibits angiogenesis by suppressing HIF-1alpha and VEGFR2 activation via ERK/p38 
      MAPK and PI3K/Akt/mTOR signaling in endothelial cells.
FAU - Kim, Gi Dae
AU  - Kim GD
AD  - Department of Food, Nutrition and Biotechnology, Kyungnam University, Gyeongnam 
      51767, Korea.
LA  - eng
PT  - Journal Article
DEP - 20171231
PL  - Korea (South)
TA  - Prev Nutr Food Sci
JT  - Preventive nutrition and food science
JID - 101586663
EIN - Prev Nutr Food Sci. 2018 Mar;23 (1):84-85. PMID: 29664481
PMC - PMC5758096
OTO - NOTNLM
OT  - HUVECS
OT  - angiogenesis
OT  - kaempferol
COIS- AUTHOR DISCLOSURE STATEMENT The author declares no conflict of interest.
EDAT- 2018/01/16 06:00
MHDA- 2018/01/16 06:01
PMCR- 2017/12/01
CRDT- 2018/01/16 06:00
PHST- 2017/10/26 00:00 [received]
PHST- 2017/11/27 00:00 [accepted]
PHST- 2018/01/16 06:00 [entrez]
PHST- 2018/01/16 06:00 [pubmed]
PHST- 2018/01/16 06:01 [medline]
PHST- 2017/12/01 00:00 [pmc-release]
AID - pnfs-22-320 [pii]
AID - 10.3746/pnf.2017.22.4.320 [doi]
PST - ppublish
SO  - Prev Nutr Food Sci. 2017 Dec;22(4):320-326. doi: 10.3746/pnf.2017.22.4.320. Epub 
      2017 Dec 31.