PMID- 29337162 OWN - NLM STAT- MEDLINE DCOM- 20180912 LR - 20210109 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 246 DP - 2018 Feb 15 TI - Antiviral activity of K22 against members of the order Nidovirales. PG - 28-34 LID - S0168-1702(17)30856-0 [pii] LID - 10.1016/j.virusres.2018.01.002 [doi] AB - Recently, a novel antiviral compound (K22) that inhibits replication of a broad range of animal and human coronaviruses was reported to interfere with viral RNA synthesis by impairing double-membrane vesicle (DMV) formation (Lundin et al., 2014). Here we assessed potential antiviral activities of K22 against a range of viruses representing two (sub)families of the order Nidovirales, the Arteriviridae (porcine reproductive and respiratory syndrome virus [PRRSV], equine arteritis virus [EAV] and simian hemorrhagic fever virus [SHFV]), and the Torovirinae (equine torovirus [EToV] and White Bream virus [WBV]). Possible effects of K22 on nidovirus replication were studied in suitable cell lines. K22 concentrations significantly decreasing infectious titres of the viruses included in this study ranged from 25 to 50 muM. Reduction of double-stranded RNA intermediates of viral replication in nidovirus-infected cells treated with K22 confirmed the anti-viral potential of K22. Collectively, the data show that K22 has antiviral activity against diverse lineages of nidoviruses, suggesting that the inhibitor targets a critical and conserved step during nidovirus replication. CI - Copyright (c) 2018 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Rappe, Julie Christiane Francoise AU - Rappe JCF AD - Institute for Virology and Immunology IVI, Mittelhausern and Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: julie.rappe@crick.ac.uk. FAU - de Wilde, Adriaan AU - de Wilde A AD - Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: A.H.de_Wilde@lumc.nl. FAU - Di, Han AU - Di H AD - Department of Biology, 623 Petit Science Center, Georgia State University, 161 Jesse Hill Jr Drive, Atlanta, GA 30303, United States. Electronic address: hdi1@gsu.edu. FAU - Muller, Christin AU - Muller C AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany. Electronic address: Christin.Mueller@viro.med.uni-giessen.de. FAU - Stalder, Hanspeter AU - Stalder H AD - Institute for Virology and Immunology IVI, Mittelhausern and Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: hanspeter.stalder@vetsuisse.unibe.ch. FAU - V'kovski, Philip AU - V'kovski P AD - Institute for Virology and Immunology IVI, Mittelhausern and Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: philip.vkovski@vetsuisse.unibe.ch. FAU - Snijder, Eric AU - Snijder E AD - Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: e.j.snijder@lumc.nl. FAU - Brinton, Margo A AU - Brinton MA AD - Department of Biology, 623 Petit Science Center, Georgia State University, 161 Jesse Hill Jr Drive, Atlanta, GA 30303, United States. Electronic address: mbrinton@gsu.edu. FAU - Ziebuhr, John AU - Ziebuhr J AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany. Electronic address: John.Ziebuhr@viro.med.uni-giessen.de. FAU - Ruggli, Nicolas AU - Ruggli N AD - Institute for Virology and Immunology IVI, Mittelhausern and Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: nicolas.ruggli@ivi.admin.ch. FAU - Thiel, Volker AU - Thiel V AD - Institute for Virology and Immunology IVI, Mittelhausern and Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: volker.thiel@vetsuisse.unibe.ch. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180111 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Antiviral Agents) RN - 0 (Benzamides) RN - 0 (Piperidines) RN - 0 (RNA, Double-Stranded) RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Antiviral Agents/*pharmacology MH - Arterivirus/*drug effects/genetics/growth & development/metabolism MH - Benzamides/*pharmacology MH - Carps MH - Cell Line MH - Chlorocebus aethiops MH - Coronaviridae/*drug effects/genetics/growth & development/metabolism MH - Epithelial Cells/drug effects/virology MH - Equartevirus/*drug effects/genetics/growth & development/metabolism MH - Mesocricetus MH - Piperidines/*pharmacology MH - Porcine respiratory and reproductive syndrome virus/*drug effects/genetics/growth & development/metabolism MH - RNA, Double-Stranded/antagonists & inhibitors/biosynthesis/genetics MH - RNA, Viral/antagonists & inhibitors/biosynthesis/genetics MH - Torovirus/*drug effects/genetics/growth & development/metabolism MH - Virus Replication/drug effects PMC - PMC7114538 OTO - NOTNLM OT - Antiviral drug OT - Double membrane vesicles OT - K22 OT - Nidoviruses OT - Replication organelles EDAT- 2018/01/18 06:00 MHDA- 2018/09/13 06:00 PMCR- 2018/01/11 CRDT- 2018/01/17 06:00 PHST- 2017/11/08 00:00 [received] PHST- 2018/01/10 00:00 [revised] PHST- 2018/01/10 00:00 [accepted] PHST- 2018/01/18 06:00 [pubmed] PHST- 2018/09/13 06:00 [medline] PHST- 2018/01/17 06:00 [entrez] PHST- 2018/01/11 00:00 [pmc-release] AID - S0168-1702(17)30856-0 [pii] AID - 10.1016/j.virusres.2018.01.002 [doi] PST - ppublish SO - Virus Res. 2018 Feb 15;246:28-34. doi: 10.1016/j.virusres.2018.01.002. Epub 2018 Jan 11.