PMID- 29338606 OWN - NLM STAT- MEDLINE DCOM- 20181018 LR - 20181018 IS - 1607-8454 (Electronic) IS - 1024-5332 (Linking) VI - 23 IP - 7 DP - 2018 Aug TI - Utility of mean sphered cell volume and mean reticulocyte volume for the diagnosis of hereditary spherocytosis. PG - 413-416 LID - 10.1080/10245332.2018.1423879 [doi] AB - INTRODUCTION: Hereditary spherocytosis (HS) is the most common congenital hemolytic anemia, characterized by anemia, jaundice, and splenomegaly. The diagnosis of HS relies on symptoms of hemolysis, a family history of HS, and a positive laboratory test which is usually the osmotic fragility test (OFT). We conducted a study to assess the utility of mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean sphered cell volume (MSCV), and mean reticulocyte volume (MRV) in the diagnosis of HS and if these are helpful in distinguishing cases of HS from immune hemolytic anemia. METHODS: A total of 102 patients suspected to have HS were enrolled. In addition 10 cases of immune hemolytic anemia (IHA) were included in the study and performance of the above screening tests was evaluated. The diagnosis of HS was based on incubated OFT, eosin 5'-maleimide (EMA) dye binding test, and flowcytometric OFT. RESULTS: A total of 29 patients were diagnosed as having HS. The sensitivity and specificity for diagnosis HS by MCHC > 35 g/dL was 44.82%, and DeltaMCV-MSCV > 10 fL has a sensitivity and specificity of 82.75% and 95.9% for diagnosis of HS. Using an algorithm of DeltaMCV-MSCV > 10 fL and DeltaMRV-MSCV < 25, for the differentiation of HS from IHA had sensitivity of 68.9% and specificity of 98.8%. FAU - Arora, Rahul Darshan AU - Arora RD AD - a Department of Hematology , Sir Ganga Ram Hospital , New Delhi , India. FAU - Dass, Jasmita AU - Dass J AD - a Department of Hematology , Sir Ganga Ram Hospital , New Delhi , India. FAU - Maydeo, Seema AU - Maydeo S AD - a Department of Hematology , Sir Ganga Ram Hospital , New Delhi , India. FAU - Arya, Vandana AU - Arya V AD - a Department of Hematology , Sir Ganga Ram Hospital , New Delhi , India. FAU - Kotwal, Jyoti AU - Kotwal J AD - a Department of Hematology , Sir Ganga Ram Hospital , New Delhi , India. FAU - Bhargava, Manorama AU - Bhargava M AD - a Department of Hematology , Sir Ganga Ram Hospital , New Delhi , India. LA - eng PT - Journal Article DEP - 20180116 PL - England TA - Hematology JT - Hematology (Amsterdam, Netherlands) JID - 9708388 SB - IM MH - Adolescent MH - Adult MH - Child MH - Child, Preschool MH - Erythrocyte Indices MH - Female MH - Flow Cytometry MH - Hemolysis MH - Humans MH - Infant MH - Infant, Newborn MH - Male MH - Middle Aged MH - *Reticulocytes MH - Sensitivity and Specificity MH - *Spherocytes MH - Spherocytosis, Hereditary/*blood/*diagnosis MH - Young Adult OTO - NOTNLM OT - Hereditary spherocytosis OT - MCHC OT - MRV OT - MSCV OT - mean reticulocyte volume OT - mean sphered cell volume OT - newer cell counter parameters OT - DeltaMCV-MSCV OT - DeltaMRV-MSCV EDAT- 2018/01/18 06:00 MHDA- 2018/10/20 06:00 CRDT- 2018/01/18 06:00 PHST- 2018/01/18 06:00 [pubmed] PHST- 2018/10/20 06:00 [medline] PHST- 2018/01/18 06:00 [entrez] AID - 10.1080/10245332.2018.1423879 [doi] PST - ppublish SO - Hematology. 2018 Aug;23(7):413-416. doi: 10.1080/10245332.2018.1423879. Epub 2018 Jan 16.