PMID- 29339208
OWN - NLM
STAT- MEDLINE
DCOM- 20190130
LR  - 20240104
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 418
DP  - 2018 Apr 1
TI  - Targeted shRNA-loaded liposome complex combined with focused ultrasound for blood 
      brain barrier disruption and suppressing glioma growth.
PG  - 147-158
LID - S0304-3835(18)30057-0 [pii]
LID - 10.1016/j.canlet.2018.01.035 [doi]
AB  - Our previous studies have demonstrated that focused ultrasound (FUS) combined 
      with DNA-loaded microbubbles (MBs) can induce noninvasive, reversible, local 
      disruption of the blood brain barrier (BBB) and enable targeted exogenous gene 
      transfer into the central nervous system. However, due to low gene loading or the 
      absence of positive targeting, to date, there has been no therapeutic effect of 
      MBs combined with FUS in tumor treatment. In the current study, we adopted a 
      phospholipid complex that exhibited sufficient gene loading and peptide-mediated 
      targeting to delay glioma growth. First, we bound MBs to shBirc5-lipo-NGR, which 
      performed the dual function of tumor cell targeting and effective gene loading. 
      Next, we demonstrated that FUS-aided MB-shBirc5-lipo-NGR exhibited a higher 
      transfection efficiency compared with the control group. Finally, we evaluated 
      the silencing effect of shBirc5 using an apoptosis assay, real time-polymerase 
      chain reaction (PCR), western blotting (WB) in vitro and a volume measurement 
      survival analysis in vivo. The experimental group exhibited a significant 
      therapeutic effect, while the FUS-only, MB-shBirc5-lipo-NGR-only and FUS-aided 
      MB-shControl-lipo-NGR groups displayed no changes in tumor growth or survival 
      time (P < .01). Consequently, our study indicated that MB-shBirc5-lipo-NGR 
      combined with FUS is a promising new RNA interference technique for the treatment 
      of glioma.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Zhao, Guanjian
AU  - Zhao G
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China; Institute of Ultrasound Imaging, The Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
FAU - Huang, Qin
AU  - Huang Q
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China.
FAU - Wang, Feng
AU  - Wang F
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China.
FAU - Zhang, Xiang
AU  - Zhang X
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China; Institute of Ultrasound Imaging, The Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
FAU - Hu, Jiangang
AU  - Hu J
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China; Institute of Ultrasound Imaging, The Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
FAU - Tan, Ying
AU  - Tan Y
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China.
FAU - Huang, Ning
AU  - Huang N
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China.
FAU - Wang, Zhigang
AU  - Wang Z
AD  - Institute of Ultrasound Imaging, The Second Affiliated Hospital of Chongqing 
      Medical University, Chongqing, 400016, China.
FAU - Wang, Zhibiao
AU  - Wang Z
AD  - Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the 
      Ministry of Science and Technology, Chongqing, 400016, China.
FAU - Cheng, Yuan
AU  - Cheng Y
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, 400010, China. Electronic address: 646872221@qq.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180112
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Contrast Media)
RN  - 0 (Liposomes)
RN  - 0 (Oligopeptides)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Survivin)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/*metabolism
MH  - Brain Neoplasms/genetics/metabolism/*therapy
MH  - Cell Line, Tumor
MH  - Contrast Media/chemistry
MH  - Drug Delivery Systems/methods
MH  - Genetic Therapy/methods
MH  - Glioma/genetics/metabolism/*therapy
MH  - Liposomes/*chemistry
MH  - Microbubbles
MH  - Oligopeptides/genetics
MH  - RNA, Small Interfering/administration & dosage/chemistry/*genetics
MH  - Rats
MH  - Sonication/*methods
MH  - Survivin/genetics
MH  - Treatment Outcome
OTO - NOTNLM
OT  - FUS
OT  - Glioma
OT  - Liposome complex
OT  - Targeted gene therapy
OT  - shRNA
EDAT- 2018/01/18 06:00
MHDA- 2019/01/31 06:00
CRDT- 2018/01/18 06:00
PHST- 2017/08/11 00:00 [received]
PHST- 2017/12/23 00:00 [revised]
PHST- 2018/01/09 00:00 [accepted]
PHST- 2018/01/18 06:00 [pubmed]
PHST- 2019/01/31 06:00 [medline]
PHST- 2018/01/18 06:00 [entrez]
AID - S0304-3835(18)30057-0 [pii]
AID - 10.1016/j.canlet.2018.01.035 [doi]
PST - ppublish
SO  - Cancer Lett. 2018 Apr 1;418:147-158. doi: 10.1016/j.canlet.2018.01.035. Epub 2018 
      Jan 12.