PMID- 29339834
OWN - NLM
STAT- MEDLINE
DCOM- 20190305
LR  - 20230216
IS  - 1530-0307 (Electronic)
IS  - 0023-6837 (Linking)
VI  - 98
IP  - 4
DP  - 2018 Apr
TI  - Use of multicolor fluorescence in situ hybridization to detect deletions in 
      clinical tissue sections.
PG  - 403-413
LID - 10.1038/s41374-017-0007-2 [doi]
AB  - A variety of laboratory methods are available for the detection of deletions of 
      tumor suppressor genes and losses of their proteins. The clinical utility of 
      fluorescence in situ hybridization (FISH) for the identification of deletions of 
      tumor suppressor genes has previously been limited by difficulties in the 
      interpretation of FISH signal patterns. The first deletion FISH assays using 
      formalin-fixed paraffin-embedded tissue sections had to deal with a significant 
      background level of signal losses affecting nuclei that are truncated by the 
      cutting process of slide preparation. Recently, more efficient probe designs, 
      incorporating probes adjacent to the tumor suppressor gene of interest, have 
      increased the accuracy of FISH deletion assays so that true chromosomal deletions 
      can be readily distinguished from the false signal losses caused by sectioning 
      artifacts. This mini-review discusses the importance of recurrent tumor 
      suppressor gene deletions in human cancer and reviews the common FISH methods 
      being used to detect the genomic losses encountered in clinical specimens. The 
      use of new probe designs to recognize truncation artifacts is illustrated with a 
      four-color PTEN FISH set optimized for prostate cancer tissue sections. Data are 
      presented to show that when section thickness is reduced, the frequency of signal 
      truncation losses is increased. We also provide some general guidelines that will 
      help pathologists and cytogeneticists run routine deletion FISH assays and 
      recognize sectioning artifacts. Finally, we summarize how recently developed 
      sequence-based approaches are being used to identify recurrent deletions using 
      small DNA samples from tumors.
FAU - Yoshimoto, Maisa
AU  - Yoshimoto M
AD  - Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada.
FAU - Ludkovski, Olga
AU  - Ludkovski O
AD  - Department of Pathology, University Health Network, Toronto, Canada.
FAU - Good, Jennifer
AU  - Good J
AD  - Department of Pathology and Molecular Medicine, Queen's University, Kingston, 
      Canada.
FAU - Pereira, Ciro
AU  - Pereira C
AD  - Departments of Genetics, School of Medicine of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto, Sao Paulo, Brazil.
FAU - Gooding, Robert J
AU  - Gooding RJ
AD  - Department of Physics, Engineering Physics and Astronomy, Queen's University, 
      Kingston, Canada.
FAU - McGowan-Jordan, Jean
AU  - McGowan-Jordan J
AD  - Genetics Diagnostic Laboratory, Children's Hospital of Eastern Ontario, Ottawa, 
      Canada.
AD  - Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, 
      Canada.
FAU - Boag, Alexander
AU  - Boag A
AD  - Department of Pathology and Molecular Medicine, Queen's University, Kingston, 
      Canada.
FAU - Evans, Andrew
AU  - Evans A
AD  - Department of Pathology, University Health Network, Toronto, Canada.
FAU - Tsao, Ming-Sound
AU  - Tsao MS
AD  - Department of Pathology, University Health Network, Toronto, Canada.
FAU - Nuin, Paulo
AU  - Nuin P
AD  - Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada.
FAU - Squire, Jeremy A
AU  - Squire JA
AUID- ORCID: 0000-0002-9863-468X
AD  - Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada. 
      jsquireinsp@gmail.com.
AD  - Department of Pathology and Molecular Medicine, Queen's University, Kingston, 
      Canada. jsquireinsp@gmail.com.
AD  - Departments of Genetics, School of Medicine of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto, Sao Paulo, Brazil. jsquireinsp@gmail.com.
AD  - Department of Pathology and Legal Medicine, School of Medicine of Ribeirao Preto, 
      University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil. 
      jsquireinsp@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20180116
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
SB  - IM
EIN - Lab Invest. 2018 Mar 8;:. PMID: 29520053
MH  - *Gene Deletion
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Neoplasms/*genetics/pathology
EDAT- 2018/01/18 06:00
MHDA- 2019/03/06 06:00
CRDT- 2018/01/18 06:00
PHST- 2017/10/11 00:00 [received]
PHST- 2017/12/01 00:00 [accepted]
PHST- 2017/12/01 00:00 [revised]
PHST- 2018/01/18 06:00 [pubmed]
PHST- 2019/03/06 06:00 [medline]
PHST- 2018/01/18 06:00 [entrez]
AID - S0023-6837(22)01122-9 [pii]
AID - 10.1038/s41374-017-0007-2 [doi]
PST - ppublish
SO  - Lab Invest. 2018 Apr;98(4):403-413. doi: 10.1038/s41374-017-0007-2. Epub 2018 Jan 
      16.