PMID- 29340375
OWN - NLM
STAT- MEDLINE
DCOM- 20180803
LR  - 20181202
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 6
IP  - 2
DP  - 2018 Jan 30
TI  - Enhancement of BMP-2-mediated angiogenesis and osteogenesis by 2-N,6-O-sulfated 
      chitosan in bone regeneration.
PG  - 431-439
LID - 10.1039/c7bm01006k [doi]
AB  - Sulfated polysaccharides are attractive semi-synthesized materials that can be 
      used as a mimic of heparan sulfate to modulate the protein activity and other 
      physiological processes. In this study, we employed sulfated chitosan to enhance 
      the angiogenic capacity of bone morphogenic protein-2 (BMP-2). Bone marrow 
      stromal cells (BMSCs) cultured in a combination of BMP-2 and 2-N,6-O-sulfated 
      chitosan (SCS) group exhibited a higher cell viability and sprouting ability. The 
      cells also secreted more VEGF and NO. The expression patterns of angiogenic and 
      osteogenic genes were analyzed, and VEGFR2 signaling was found to play a role in 
      the enhancing effect of SCS. The chick embryo chorioallantoic membrane (CAM) 
      assay revealed an enhanced angiogenic effect of BMP-2 when SCS was involved. In 
      addition, we investigated angiogenesis and osteogenesis in mouse using a 
      BMP-2-induced ectopic bone model. Histological and immune-staining analysis 
      revealed that both bone and vascular tissue were enhanced when SCS was added. 
      These data prove that SCS can improve the angiogenic potential of BMP-2 and thus 
      lead to better bone regeneration.
FAU - Pan, Yuanzhong
AU  - Pan Y
AD  - The State Key Laboratory of Bioreactor Engineering, East China University of 
      Science and Technology, Shanghai, 200237 People's Republic of China. 
      biomatwj@163.com liucs@ecust.edu.cn.
FAU - Chen, Jie
AU  - Chen J
FAU - Yu, Yuanman
AU  - Yu Y
FAU - Dai, Kai
AU  - Dai K
FAU - Wang, Jing
AU  - Wang J
FAU - Liu, Changsheng
AU  - Liu C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (2-N, 6-O-sulfated chitosan)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects/metabolism
MH  - Bone Morphogenetic Protein 2/*pharmacology
MH  - *Bone Regeneration
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Chitosan/*analogs & derivatives/pharmacology
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Mice
MH  - *Neovascularization, Physiologic
MH  - Nitric Oxide/metabolism
MH  - *Osteogenesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2018/01/18 06:00
MHDA- 2018/08/04 06:00
CRDT- 2018/01/18 06:00
PHST- 2018/01/18 06:00 [pubmed]
PHST- 2018/08/04 06:00 [medline]
PHST- 2018/01/18 06:00 [entrez]
AID - 10.1039/c7bm01006k [doi]
PST - ppublish
SO  - Biomater Sci. 2018 Jan 30;6(2):431-439. doi: 10.1039/c7bm01006k.