PMID- 29343880
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240313
IS  - 0044-5991 (Print)
IS  - 1347-5800 (Electronic)
IS  - 0044-5991 (Linking)
VI  - 50
IP  - 6
DP  - 2017 Dec 26
TI  - Novel Application of Loop-mediated Isothermal Amplification for Rapid Detection 
      of Gene Translocation.
PG  - 169-176
LID - 10.1267/ahc.17024 [doi]
AB  - Identification of fusion genes in cancer is essential for pathological diagnosis 
      and clinical therapy. Although methods for detection of fusion genes, such as 
      fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction 
      (PCR), have been developed in last two decades, these methods are not ideal for 
      detection of these genetic alterations owing to their high cost and 
      time-consuming procedures. In this study, we developed novel application for 
      detection of gene translocations using loop-mediated isothermal amplification 
      (LAMP). We verified the amplified DNA products of echinoderm 
      microtubule-associated protein-like 4 and anaplastic lymphoma kinase (EML4-ALK), 
      synaptotagmin and synovial sarcoma, X breakpoint (SYT-SSX), and immunoglobulin 
      heavy chain gene and B cell leukemia/lymphoma 2 (IgH/BCL2) by real-time PCR, 
      agarose-gel electrophoresis, and the naked eye after the LAMP procedure. Fusion 
      genes were detected in samples diluted 10(3) times within 60 min. Because of the 
      advantages of rapid amplification, simple operation, and easy detection without 
      requiring sophisticated equipment or technical skill, LAMP may have potential 
      applications as an on-site analytical approach in hospitals for pathological 
      diagnosis and decision making regarding appropriate therapeutic approachs.
FAU - Matsuzaki, Ibu
AU  - Matsuzaki I
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Iguchi, Hideto
AU  - Iguchi H
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Mikasa, Yurina
AU  - Mikasa Y
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Morishita, Hiromu
AU  - Morishita H
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Okuda, Katsuya
AU  - Okuda K
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Nakaguchi, Keita
AU  - Nakaguchi K
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Mori, Yuki
AU  - Mori Y
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Iwahashi, Yoshifumi
AU  - Iwahashi Y
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Warigaya, Kenji
AU  - Warigaya K
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Fujimoto, Masakazu
AU  - Fujimoto M
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Kojima, Fumiyoshi
AU  - Kojima F
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Murata, Shin-Ichi
AU  - Murata SI
AD  - Department of Human Pathology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
LA  - eng
PT  - Journal Article
DEP - 20171207
PL  - Japan
TA  - Acta Histochem Cytochem
JT  - Acta histochemica et cytochemica
JID - 0147110
PMC - PMC5765217
OTO - NOTNLM
OT  - X breakpoint
OT  - echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase; 
      synaptotagmin and synovial sarcoma
OT  - gene translocation
OT  - immunoglobulin heavy chain gene and B cell leukemia/lymphoma 2
OT  - loop-mediated isothermal amplification
EDAT- 2018/01/19 06:00
MHDA- 2018/01/19 06:01
PMCR- 2017/12/26
CRDT- 2018/01/19 06:00
PHST- 2017/08/23 00:00 [received]
PHST- 2017/11/06 00:00 [accepted]
PHST- 2018/01/19 06:00 [entrez]
PHST- 2018/01/19 06:00 [pubmed]
PHST- 2018/01/19 06:01 [medline]
PHST- 2017/12/26 00:00 [pmc-release]
AID - JST.JSTAGE/ahc/17024 [pii]
AID - 10.1267/ahc.17024 [doi]
PST - ppublish
SO  - Acta Histochem Cytochem. 2017 Dec 26;50(6):169-176. doi: 10.1267/ahc.17024. Epub 
      2017 Dec 7.