PMID- 29344181
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 14
IP  - 6
DP  - 2017 Dec
TI  - L-type amino acid transporter 1 expression is upregulated and associated with 
      cellular proliferation in colorectal cancer.
PG  - 7410-7416
LID - 10.3892/ol.2017.7148 [doi]
AB  - Previous studies have shown that the L-type amino acid transporter 1 (LAT1) is 
      highly expressed in many types of cancer. Upregulated LAT1 expression is 
      considered to be associated with cancer cell proliferation. In the present study, 
      we investigated LAT1 expression in 210 patients with colorectal cancer (CRC) and 
      40 patients with colonic adenoma using an immunohistochemical method, and 
      analyzed the associations between LAT1 expression and clinicopathological factors 
      and prognosis. The biological significance of LAT1 was also examined under 
      conditions with sub-normal amounts of essential amino acids using colon cancer 
      cell lines. High expression of LAT1 was observed in 152 of 210 CRC patients 
      (72.4%) and 12 of 40 patients with colonic adenoma (30%), and this difference in 
      the frequency of LAT1 expression between CRC and adenoma was significant 
      (P<0.001). High expression of LAT1 was associated with venous invasion (P=0.027). 
      The restriction of amino acids suppressed cell proliferation in CRC cells with 
      higher LAT1 expression, while cellular proliferation was not suppressed in the 
      cells expressing lower levels of LAT1. Mammalian target of rapamycin (mTOR) 
      expression was also downregulated under restricted availability of amino acids, 
      suggesting that the restriction of amino acids induced an antitumor effect 
      through inhibition of the LAT1/mTOR pathway. In summary, the present study 
      demonstrated that LAT1 expression is frequently upregulated in CRC and is 
      associated with cancer cell proliferation via the mTOR pathway.
FAU - Hayase, Suguru
AU  - Hayase S
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
FAU - Kumamoto, Kensuke
AU  - Kumamoto K
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
AD  - Department of Coloproctology, Aizu Medical Center, Fukushima Medical University, 
      Aizuwakamatsu, Fukushima 969-3492, Japan.
FAU - Saito, Katsuharu
AU  - Saito K
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
FAU - Kofunato, Yasuhide
AU  - Kofunato Y
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
FAU - Sato, Yu
AU  - Sato Y
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
FAU - Okayama, Hirokazu
AU  - Okayama H
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
FAU - Miyamoto, Kotaro
AU  - Miyamoto K
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
FAU - Ohki, Shinji
AU  - Ohki S
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
FAU - Takenoshita, Seiichi
AU  - Takenoshita S
AD  - Department of Organ Regulatory Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Fukushima 960-1295, Japan.
LA  - eng
PT  - Journal Article
DEP - 20171006
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5755048
OTO - NOTNLM
OT  - L-type amino acid transporter 1
OT  - colorectal cancer
OT  - mammalian target of rapamycin
EDAT- 2018/01/19 06:00
MHDA- 2018/01/19 06:01
PMCR- 2017/10/06
CRDT- 2018/01/19 06:00
PHST- 2017/04/17 00:00 [received]
PHST- 2017/09/27 00:00 [accepted]
PHST- 2018/01/19 06:00 [entrez]
PHST- 2018/01/19 06:00 [pubmed]
PHST- 2018/01/19 06:01 [medline]
PHST- 2017/10/06 00:00 [pmc-release]
AID - OL-0-0-7148 [pii]
AID - 10.3892/ol.2017.7148 [doi]
PST - ppublish
SO  - Oncol Lett. 2017 Dec;14(6):7410-7416. doi: 10.3892/ol.2017.7148. Epub 2017 Oct 6.