PMID- 29344493
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220311
IS  - 2296-4681 (Print)
IS  - 2296-4657 (Electronic)
IS  - 2296-4657 (Linking)
VI  - 4
IP  - 1
DP  - 2017 Dec
TI  - Comparison of Gene Expression Profiling and Chromosome 3 Analysis by Fluorescent 
      in situ Hybridization and Multiplex Ligation Probe Amplification in Fine-Needle 
      Aspiration Biopsy Specimens of Uveal Melanoma.
PG  - 16-20
LID - 10.1159/000468941 [doi]
AB  - PURPOSE: The aim of this paper was to assess the concordance between results of 
      DecisionDx-UM specific gene expression profiling (GEP) and fluorescence in situ 
      hybridization (FISH) for chromosome 3 analysis, and between DecisionDx-UM GEP and 
      multiplex ligation probe amplification (MLPA) in uveal melanoma undergoing 
      intraoperative fine-needle aspiration biopsy (FNAB) for metastatic 
      prognostication during brachytherapy. METHODS: We retrospectively reviewed 
      consecutive patients diagnosed with posterior uveal melanoma who underwent 
      intraoperative FNAB prior to placement of an iodine-125 radioactive plaque 
      between 2012 and 2014. Two cohorts of patients were identified: Cohort 1 - tumors 
      in which both GEP and FISH results were obtained, and Cohort 2 - tumors in which 
      both GEP and MLPA results were obtained. RESULTS: Forty-four patients were 
      identified for Cohort 1. FISH and GEP results were discordant in 7 tumors 
      (15.9%). Forty-three patients were identified for Cohort 2. MLPA and GEP were 
      discordant in 7 tumors (16.3%). CONCLUSIONS: Discordance between GEP and 
      chromosome 3 status by FISH and MLPA occurred in our series at a rate of 15.9 and 
      16.3%, respectively. Caution must be advised when counseling a patient with a 
      good-prognosis GEP "Class 1" result that the uveal tumor may actually harbor 
      monosomy 3, which is associated with a poor prognosis for metastasis in nearly 
      20% of the patients.
FAU - Klufas, Michael A
AU  - Klufas MA
AD  - Department of Ophthalmology at Stein Eye Institute, University of California, Los 
      Angeles, CA, USA.
FAU - Richter, Elizabeth
AU  - Richter E
AD  - Department of Ophthalmology at Stein Eye Institute, University of California, Los 
      Angeles, CA, USA.
FAU - Itty, Sujit
AU  - Itty S
AD  - Department of Ophthalmology at Stein Eye Institute, University of California, Los 
      Angeles, CA, USA.
FAU - Moreno, Christian
AU  - Moreno C
AD  - Department of Ophthalmology at Stein Eye Institute, University of California, Los 
      Angeles, CA, USA.
FAU - McCannel, Colin A
AU  - McCannel CA
AD  - Department of Ophthalmology at Stein Eye Institute, University of California, Los 
      Angeles, CA, USA.
AD  - Department of Ophthalmology at Doheny Eye Institute, University of California, 
      Los Angeles, CA, USA.
FAU - McCannel, Tara A
AU  - McCannel TA
AD  - Department of Ophthalmology at Stein Eye Institute, University of California, Los 
      Angeles, CA, USA.
AD  - Department of Ophthalmology at Doheny Eye Institute, University of California, 
      Los Angeles, CA, USA.
LA  - eng
PT  - Journal Article
DEP - 20170614
PL  - Switzerland
TA  - Ocul Oncol Pathol
JT  - Ocular oncology and pathology
JID - 101656139
PMC - PMC5757548
OTO - NOTNLM
OT  - Choroidal melanoma
OT  - Fine-needle aspiration biopsy
OT  - Fluorescent in situ hybridization
OT  - Gene expression profiling
OT  - Molecular prognostication
OT  - Monosomy 3
OT  - Multiplex ligation probe amplification
OT  - Uveal melanoma
EDAT- 2018/01/19 06:00
MHDA- 2018/01/19 06:01
PMCR- 2018/12/01
CRDT- 2018/01/19 06:00
PHST- 2016/12/14 00:00 [received]
PHST- 2017/03/01 00:00 [revised]
PHST- 2018/01/19 06:00 [entrez]
PHST- 2018/01/19 06:00 [pubmed]
PHST- 2018/01/19 06:01 [medline]
PHST- 2018/12/01 00:00 [pmc-release]
AID - oop-0004-0016 [pii]
AID - 10.1159/000468941 [doi]
PST - ppublish
SO  - Ocul Oncol Pathol. 2017 Dec;4(1):16-20. doi: 10.1159/000468941. Epub 2017 Jun 14.