PMID- 2934476
OWN - NLM
STAT- MEDLINE
DCOM- 19860218
LR  - 20051117
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 136
IP  - 3
DP  - 1986 Feb 1
TI  - Recycling of receptor-bound IgE by rat basophilic leukemia cells.
PG  - 1015-22
AB  - Rat basophilic leukemia (RBL) cells have receptors for immunoglobulin E (IgE). We 
      previously showed that unlike some other ligands, the binding of monomeric rat or 
      mouse IgE to RBL cells does not induce endocytosis. However, aggregation of the 
      cell-bound, monomeric mouse IgE anti-dinitrophenyl (DNP) with DNP-protein 
      conjugates leads to endocytosis of the aggregated mouse IgE and to the 
      co-endocytosis of some unaggregated monomeric rat IgE. In this study we analyzed 
      and compared the fate of co-endocytosed and endocytosed IgE. We found that 
      co-endocytosed rat IgE recycled back to the cell surface within 3 to 4 hr. In 
      contrast, endocytosed, immunochemically cross-linked, receptor-bound mouse IgE 
      anti-DNP was partially degraded and was released into the medium, with no 
      observable recycling of receptors, by 3 hr. However, addition of the hapten, 
      DNP-lysine, resulted in rapid recycling (t1/2 10 min) of the endocytosed 
      receptor-bound IgE to the plasma membrane and blocked additional endocytosis. 
      Recycling of the endocytosed mouse IgE was more pronounced when the hapten was 
      added to cells within 20 min of the initiation of endocytosis. When the hapten 
      was added to the cells at later times (60 to 180 min), progressively less IgE 
      recycled to the surface. This may reflect shuttling of the internalized IgE from 
      a 'prelysosomal' to a 'lysosomal' compartment. Thus we provide evidence for 
      recycling of monomeric IgE receptor complexes, sorting between cross-linked and 
      non-cross-linked IgE receptor complexes, the freeing of receptor-bound monomeric 
      IgE from the endocytosed immune-complexed IgE, and the apparent dependence of the 
      recycling efficiency upon intracellular localization.
FAU - Furuichi, K
AU  - Furuichi K
FAU - Rivera, J
AU  - Rivera J
FAU - Buonocore, L M
AU  - Buonocore LM
FAU - Isersky, C
AU  - Isersky C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Dinitrophenols)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Serum Albumin)
RN  - 0 (dinitrophenyl-human serum albumin conjugate)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antigen-Antibody Complex/metabolism
MH  - Basophils/metabolism
MH  - Cell Line
MH  - Cross-Linking Reagents
MH  - Dinitrobenzenes/immunology
MH  - Dinitrophenols
MH  - *Endocytosis
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia/*metabolism
MH  - Rats
MH  - Receptors, Antigen, B-Cell/metabolism
MH  - Receptors, Fc/*metabolism
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism
MH  - Serum Albumin
EDAT- 1986/02/01 00:00
MHDA- 1986/02/01 00:01
CRDT- 1986/02/01 00:00
PHST- 1986/02/01 00:00 [pubmed]
PHST- 1986/02/01 00:01 [medline]
PHST- 1986/02/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1986 Feb 1;136(3):1015-22.