PMID- 29344825
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20181113
IS  - 1436-2236 (Electronic)
IS  - 1436-2228 (Linking)
VI  - 20
IP  - 1
DP  - 2018 Feb
TI  - The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas 
      Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an 
      Indicator of the Warburg Effect.
PG  - 87-97
LID - 10.1007/s10126-017-9789-x [doi]
AB  - Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC 
      drives cellular energy metabolism by controlling the influx and efflux of 
      metabolites and ions through the mitochondrial membrane, playing a role in its 
      permeabilization. This protein exerts a pivotal role during the white spot 
      syndrome virus (WSSV) infection in shrimp, through its involvement in a 
      particular metabolism that plays in favor of the virus, the Warburg effect. The 
      Warburg effect corresponds to an atypical metabolic shift toward an aerobic 
      glycolysis that provides energy for rapid cell division and resistance to 
      apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs 
      during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in 
      the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed 
      a specific antibody directed against C. gigas VDAC. This tool allowed us to 
      quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to 
      follow the amount of VDAC in oysters deployed in the field. We showed that 
      oysters sensitive to a mortality event in the field presented an accumulation of 
      VDAC. Finally, we propose to use VDAC quantification as a tool to measure the 
      oyster susceptibility to OsHV-1 depending on its environment.
FAU - Delisle, Lizenn
AU  - Delisle L
AUID- ORCID: 0000-0003-2920-208X
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de 
      l'Environnement Marin (LEMAR), 29280, Plouzane, France. 
      Lizenn.Delisle@ifremer.fr.
AD  - Ifremer, Laboratoire de physiologie des invertebres (LPI), Unite de physiologie 
      fonctionnelle des organismes marins (PFOM), Centre Ifremer de Bretagne, 1625 
      Route de Saint Anne, CS 10070, 29280, Plouzane, France. 
      Lizenn.Delisle@ifremer.fr.
FAU - Fuhrmann, Marine
AU  - Fuhrmann M
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de 
      l'Environnement Marin (LEMAR), 29280, Plouzane, France.
FAU - Quere, Claudie
AU  - Quere C
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de 
      l'Environnement Marin (LEMAR), 29280, Plouzane, France.
FAU - Pauletto, Marianna
AU  - Pauletto M
AD  - Department of Comparative Biomedicine and Food Science, University of Padova, 
      Viale dell'Universita 16, 35020 Legnaro, Padova, Italy.
FAU - Pichereau, Vianney
AU  - Pichereau V
AD  - Universite de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire 
      des sciences de l'Environnement Marin (LEMAR), 29280, Plouzane, France.
FAU - Pernet, Fabrice
AU  - Pernet F
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de 
      l'Environnement Marin (LEMAR), 29280, Plouzane, France.
FAU - Corporeau, Charlotte
AU  - Corporeau C
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de 
      l'Environnement Marin (LEMAR), 29280, Plouzane, France.
LA  - eng
PT  - Journal Article
DEP - 20180117
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (Antibodies)
RN  - 0 (Voltage-Dependent Anion Channels)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Apoptosis
MH  - Crassostrea/metabolism/*virology
MH  - Herpesviridae/*metabolism
MH  - Herpesviridae Infections/*metabolism/physiopathology
MH  - Sequence Analysis, Protein
MH  - Voltage-Dependent Anion Channels/*chemistry/immunology
OTO - NOTNLM
OT  - Crassostrea gigas
OT  - Ostreid herpes virus
OT  - Voltage-dependent anion channel
OT  - Warburg effect
EDAT- 2018/01/19 06:00
MHDA- 2018/11/09 06:00
CRDT- 2018/01/19 06:00
PHST- 2017/07/12 00:00 [received]
PHST- 2017/12/07 00:00 [accepted]
PHST- 2018/01/19 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/01/19 06:00 [entrez]
AID - 10.1007/s10126-017-9789-x [pii]
AID - 10.1007/s10126-017-9789-x [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2018 Feb;20(1):87-97. doi: 10.1007/s10126-017-9789-x. Epub 
      2018 Jan 17.