PMID- 29345295
OWN - NLM
STAT- MEDLINE
DCOM- 20180806
LR  - 20180816
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 52
IP  - 2
DP  - 2018 Feb
TI  - Upregulation of the BDNF/TrKB pathway promotes epithelial-mesenchymal transition, 
      as well as the migration and invasion of cervical cancer.
PG  - 461-472
LID - 10.3892/ijo.2017.4230 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) has previously been demonstrated to be 
      associated with several types of cancer. In addition, its receptor, tropomyosin 
      related kinase B (TrkB) is involved in tumor invasion and metastasis. 
      Epithelial-mesenchymal transition (EMT) is associated with metastasis in cancers. 
      Thus, The aim of the present study was to examine whether BDNF/TrKB expression is 
      linked to a poor survival and the acquisition of the EMT phenotype in cervical 
      cancer. We found that a high positive expression of BDNF/TrKB was associated with 
      poor survival in cervical cancer. Our results revealed that high expression 
      levels of BDNF/TrKB were observed in cervical cancer compared to normal cells. 
      Importantly, we demonstrated that the silencing of TrKB suppressed the activation 
      of EMT via the downregulation of N-cadherin, vimentin, matrix 
      metalloproteinase (MMP)2 and MMP9, and the upregulation of E-cadherin and tissue 
      inhibitor of metalloproteinases (TIMP)2, which resulted in suppressed cell 
      proliferation, migration and invasion. Furthermore, high phosphorylation levels 
      of ERK and Akt were observed in the cervical cancer cells, while these levels 
      were decreased in the cells in which TrKB was knocked down. On the whole, these 
      findings suggest that the BDNF/TrKB pathway is a promising target for the 
      prevention of tumor proliferation, invasion, metastasis and EMT in cervical 
      cancer cells.
FAU - Yuan, Yuan
AU  - Yuan Y
AD  - Department of Gynecology, The Affiliated Hospital of Southwest Medical 
      University, Luzhou, Sichuan 646000, P.R. China.
FAU - Ye, Hai-Qiong
AU  - Ye HQ
AD  - Department of Gynecology, The Affiliated Hospital of Southwest Medical 
      University, Luzhou, Sichuan 646000, P.R. China.
FAU - Ren, Qian-Chuan
AU  - Ren QC
AD  - Department of Gynecology, The Affiliated Hospital of Southwest Medical 
      University, Luzhou, Sichuan 646000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171219
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Membrane Glycoproteins)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (tropomyosin-related kinase-B, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Epithelial-Mesenchymal Transition/genetics/physiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice, Inbred BALB C
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Receptor, trkB/genetics/*metabolism
MH  - Signal Transduction
MH  - Up-Regulation
MH  - Uterine Cervical Neoplasms/metabolism/*mortality/*pathology
EDAT- 2018/01/19 06:00
MHDA- 2018/08/07 06:00
CRDT- 2018/01/19 06:00
PHST- 2017/08/31 00:00 [received]
PHST- 2017/12/01 00:00 [accepted]
PHST- 2018/01/19 06:00 [entrez]
PHST- 2018/01/19 06:00 [pubmed]
PHST- 2018/08/07 06:00 [medline]
AID - 10.3892/ijo.2017.4230 [doi]
PST - ppublish
SO  - Int J Oncol. 2018 Feb;52(2):461-472. doi: 10.3892/ijo.2017.4230. Epub 2017 Dec 
      19.