PMID- 29345353
OWN - NLM
STAT- MEDLINE
DCOM- 20190227
LR  - 20190227
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 119
IP  - 6
DP  - 2018 Jun
TI  - HipOP mesenchymal population has high potential for repairing injured peripheral 
      nerves.
PG  - 4836-4844
LID - 10.1002/jcb.26684 [doi]
AB  - Bone marrow stromal cells (BMSCs) are reportedly a heterogeneous population of 
      mesenchymal stem cells (MSCs). Recently, we developed a simple strategy for the 
      enrichment of MSCs with the capacity to differentiate into osteoblasts, 
      chondrocytes, and adipocytes. On transplantation, the progenitor-enriched 
      fractions can regenerate the bone with multiple lineages of donor origin and are 
      thus called "highly purified osteoprogenitors" (HipOPs). Although our previous 
      studies have demonstrated that HipOPs are enriched with MSCs and exhibit a higher 
      potential to differentiate into osteoblasts, adipocytes, and chondrocytes than 
      BMSCs, their potential to differentiate into neural cells has not been clarified. 
      In this study, we evaluated the efficacy of HipOPs as a resource of neural stem 
      cells. The neurosphere assay showed that neurospheres formed by HipOPs exhibited 
      self-renewal ability and their size was generally larger than that of 
      neurospheres formed by BMSCs. A limiting dilution assay was used to evaluate the 
      frequency of neural progenitors in BMSCs and HipOPs. The results demonstrated 
      that the frequency of neural progenitors in HipOPs was 120-fold higher than that 
      in BMSCs. Furthermore, to investigate the in vivo regenerative potential of the 
      peripheral nerve, we modified a murine peripheral nerve injury experimental model 
      and demonstrated that HipOPs exhibit a higher efficacy in repairing injured 
      peripheral nerves. These findings suggest that HipOPs are a useful cell resource 
      for regenerative therapies such as that in case of peripheral nerve injury.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Yamauchi, Yukako
AU  - Yamauchi Y
AD  - Department of Restorative Dentistry and Endodontology, Osaka University Graduate 
      School of Dentistry, Osaka, Japan.
FAU - Itoh, Shousaku
AU  - Itoh S
AUID- ORCID: 0000-0002-1440-2495
AD  - Department of Restorative Dentistry and Endodontology, Osaka University Graduate 
      School of Dentistry, Osaka, Japan.
FAU - Naruse, Haruna
AU  - Naruse H
AD  - Department of Restorative Dentistry and Endodontology, Osaka University Graduate 
      School of Dentistry, Osaka, Japan.
FAU - Itoh, Yuki
AU  - Itoh Y
AD  - Department of Restorative Dentistry and Endodontology, Osaka University Graduate 
      School of Dentistry, Osaka, Japan.
FAU - Abe, Makoto
AU  - Abe M
AD  - Department of Oral Anatomy and Developmental Biology, Osaka University Graduate 
      School of Dentistry, Osaka, Japan.
FAU - Kagioka, Takumi
AU  - Kagioka T
AD  - Department of Restorative Dentistry and Endodontology, Osaka University Graduate 
      School of Dentistry, Osaka, Japan.
FAU - Hayashi, Mikako
AU  - Hayashi M
AD  - Department of Restorative Dentistry and Endodontology, Osaka University Graduate 
      School of Dentistry, Osaka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180301
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
SB  - IM
MH  - Allografts
MH  - Animals
MH  - Bone Marrow Cells/*metabolism/pathology
MH  - Female
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism/pathology
MH  - Mice
MH  - Neural Stem Cells/*metabolism/pathology
MH  - Peripheral Nerve Injuries/metabolism/*therapy
OTO - NOTNLM
OT  - bone marrow transplantation
OT  - mesenchymal stem cell
OT  - sciatic nerve injury
EDAT- 2018/01/19 06:00
MHDA- 2019/02/28 06:00
CRDT- 2018/01/19 06:00
PHST- 2017/11/02 00:00 [received]
PHST- 2018/01/17 00:00 [accepted]
PHST- 2018/01/19 06:00 [pubmed]
PHST- 2019/02/28 06:00 [medline]
PHST- 2018/01/19 06:00 [entrez]
AID - 10.1002/jcb.26684 [doi]
PST - ppublish
SO  - J Cell Biochem. 2018 Jun;119(6):4836-4844. doi: 10.1002/jcb.26684. Epub 2018 Mar 
      1.