PMID- 29350446
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20190110
IS  - 1532-2149 (Electronic)
IS  - 1090-3801 (Linking)
VI  - 22
IP  - 5
DP  - 2018 May
TI  - Differential gene expression in trigeminal ganglia of male and female rats 
      following chronic constriction of the infraorbital nerve.
PG  - 875-888
LID - 10.1002/ejp.1174 [doi]
AB  - BACKGROUND: The mechanisms underlying sex-based differences in pain and analgesia 
      are poorly understood. In this study, we investigated gene expression changes in 
      trigeminal ganglia (TG) of male and female rats exposed to infraorbital nerve 
      chronic constriction injury (IoN-CCI). METHODS: Somatosensory assessments were 
      performed prior to IoN-CCI and at selected time points postsurgery. Selected gene 
      expression changes were examined with real-time quantitative polymerase chain 
      reaction (RT-PCR) in ipsilateral TG at 21 days postsurgery. RESULTS: Rats exposed 
      to IoN-CCI developed significant mechanical allodynia and hyperalgesia on days 19 
      and 21 postsurgery. During this period, females developed significantly more 
      allodynia but not hyperalgesia compared to males. At 21 days postsurgery, 
      expression levels of 44 of the 84 investigated pain-related genes in ipsilateral 
      TG were significantly regulated relative to naive rats in either sex. Csf1 and 
      Cx3cr1 were up-regulated in both sexes, but the magnitude of regulation was 
      significantly higher in females (p = 0.02 and p = 0.001, respectively). Htr1a and 
      Scn9a were down-regulated in both sexes, but the down-regulation was 
      significantly more pronounced in males (p = 0.04 and p = 0.02, respectively). 
      Additionally, Cck, Il1a, Pla2g1b and Tnf genes were significantly regulated in 
      females but not in males, and Chrna4 gene was significantly down-regulated in 
      males but not in females. CONCLUSIONS: Our findings suggest sex-dependent gene 
      regulation in response to nerve injury, which may contribute to sex dimorphism of 
      trigeminal neuropathic pain. Further studies are needed to establish gene 
      expression changes over time and correlate these with hormonal and other 
      physiological parameters in male and female. SIGNIFICANCE: We present novel 
      sex-specific transcriptional regulation in trigeminal ganglia that may contribute 
      to male-/female-based differences in trigeminal neuropathic pain. These findings 
      are expected to open new research horizons, particularly in male versus female 
      targeted therapeutic regimens.
CI  - (c) 2018 European Pain Federation - EFIC(R).
FAU - Korczeniewska, O A
AU  - Korczeniewska OA
AD  - Department of Diagnostic Sciences, Rutgers School of Dental Medicine, Rutgers, 
      The State University of New Jersey, Newark, NJ, USA.
FAU - Husain, S
AU  - Husain S
AD  - The Genomics Center, New Jersey Medical School, Rutgers, The State University of 
      New Jersey, Newark, NJ, USA.
FAU - Khan, J
AU  - Khan J
AD  - Eastman Institute for Oral Health, University of Rochester Medical Center, 
      Rochester, NY, USA.
FAU - Eliav, E
AU  - Eliav E
AD  - Eastman Institute for Oral Health, University of Rochester Medical Center, 
      Rochester, NY, USA.
FAU - Soteropoulos, P
AU  - Soteropoulos P
AD  - The Genomics Center, New Jersey Medical School, Rutgers, The State University of 
      New Jersey, Newark, NJ, USA.
FAU - Benoliel, R
AU  - Benoliel R
AD  - Department of Diagnostic Sciences, Rutgers School of Dental Medicine, Rutgers, 
      The State University of New Jersey, Newark, NJ, USA.
LA  - eng
PT  - Journal Article
DEP - 20180119
PL  - England
TA  - Eur J Pain
JT  - European journal of pain (London, England)
JID - 9801774
SB  - IM
MH  - Animals
MH  - Female
MH  - *Gene Expression
MH  - Hyperalgesia/*genetics/metabolism
MH  - Male
MH  - Nerve Compression Syndromes/*genetics/metabolism
MH  - Pain Measurement
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Sex Characteristics
MH  - Trigeminal Ganglion/*metabolism
EDAT- 2018/01/20 06:00
MHDA- 2019/01/11 06:00
CRDT- 2018/01/20 06:00
PHST- 2017/12/10 00:00 [accepted]
PHST- 2018/01/20 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
PHST- 2018/01/20 06:00 [entrez]
AID - 10.1002/ejp.1174 [doi]
PST - ppublish
SO  - Eur J Pain. 2018 May;22(5):875-888. doi: 10.1002/ejp.1174. Epub 2018 Jan 19.